Sedimentological processes are underexplored in retro-arc basin segments of source-to-sink systems, particularly in the distal back-bulge depozone. The extant Pantanal Basin of Latin America is a large sub-tropical continental back-bulge for which the boundary conditions on riverine sedimentation (tectonics, climate, phytogeography) are reasonably understood. Here, we use a new database of riverbed sand samples (n = 97) to investigate the modern provenance of the Pantanal Basin. Petrographic analysis of grain-mounted thin sections and Gazzi-Dickinson point counting were used to assess the framework composition of the sands, and a pour point analysis based on geographic information systems (GIS) defined the hinterland geological environment for each sample. Six provenance domains were defined: (a) lowlands, (b) Amazon craton, (c) Rio Apa craton, (d) plateau, (e) South Paraguay Belt, and (f) North Paraguay Belt. Most Pantanal samples are quartzose non-orogenic detritus (Qt88\F5\L7). Rivers draining the Paraguay Belt highlands carry more lithic grains derived from carbonate parent rocks. The occurrence of plagioclase grains is rare. However, river sands where >30% of the total assemblage was K-feldspar were present downstream of foliated metamorphic outcrops of the Rio Apa craton. Sand relatively rich in fine K-feldspar grains in the medial Taquari River megafan were attributed to channel avulsion, incision, and reworking of floodplain deposits. Statistical analysis suggests that bedrock lithology and average annual rainfall are important controls on fluvial sands in the Pantanal Basin, with watershed slope, temperature, and dilution playing secondary roles in the spatial distribution of dominant grain types. This study provides new actualistic data that aim to improve interpretations of the geological record.