Hippocampus kuda protein hydrolysate improves male reproductive dysfunction in diabetic rats

Sudirman, Sabri; Su, Chieh-Yu; Tsou, David; Lee, Meng–Chou; Kong, Zwe‐Ling

doi:10.1016/j.biopha.2021.111760

Cited by 11 publications

(8 citation statements)

References 48 publications

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“…The hydrolysate was dried by using a freeze dryer and stored at -20°C for further analysis. A previous study reported that the protein content of this hydrolysate was about 6.23 mg BSA equivalent/g dried sample with 29.04% of the degree of hydrolysis, and two major protein bands were found at 33 kDa and 63 kDa as analyzed by using a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…Seahorse hydrolysate also inhibits the intracellular reactive oxygen species level and Rac1 activation of 12-O-tetradecanoylphorbol-13-acetate- (TPA-)-induced MG-63 cells [ 14 ]. Recently, a previous study reported that Hippocampus kuda protein hydrolysate improves male reproductive dysfunction in diabetic rats [ 15 ]. However, the effect of seahorse protein hydrolysate on the OA model of obesity rats has not been reported.…”

Section: Introductionmentioning

confidence: 99%

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Seahorse Protein Hydrolysate Ameliorates Proinflammatory Mediators and Cartilage Degradation on Posttraumatic Osteoarthritis with an Obesity Rat Model

Sudirman

Tseng

Chen

et al. 2022

BioMed Research International

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Osteoarthritis (OA) is one of the age-related diseases and is highly present on the knees. Obesity and mechanical injuries as a risk factor of OA are attributed to cartilage disintegration, joint loading, and inflammation. This study is aimed at investigating the effects of seahorse protein hydrolysate (SH) on posttraumatic osteoarthritis in an obesity rat. The OA model was developed by anterior cruciate ligament transection with medial meniscectomy in a high-fat diet- (HFD-) induced obesity rat model. The male Sprague-Dawley rats were fed a HFD for 6 weeks before OA surgery. The OA rats were treated with oral gavage by 4, 8, or 20 mg/kg of body weight of SH for 6 weeks of treatment. The expressions of plasma proinflammatory factors, C-telopeptide of type II collagen, and matrix metalloproteinase- (MMP-) 3 and MMP-13 were reduced by SH treatment. Plasma superoxide dismutase and glutathione peroxidase activities were enhanced by SH. SH also relieved the pain of the knee joint and swelling as well as decreased proteoglycan loss in the knee articular cartilage caused by osteoarthritis. Based on these results, SH suppressed proinflammatory factors and attenuated cartilage degradation and pain in the OA model. Therefore, seahorse protein hydrolysate might be a potential opportunity for improving the development of osteoarthritis.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Seahorse Protein Hydrolysate Ameliorates Proinflammatory Mediators and Cartilage Degradation on Posttraumatic Osteoarthritis with an Obesity Rat Model

Sudirman

Tseng

Chen

et al. 2022

BioMed Research International

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“…Park et al [ 16 ] reported an increase in serum testosterone levels in mice with seahorse. Another study reported the hydrolysate protein of seahorse ( Hippocampus kuda ) amelioration effects on diabetic rats with reproductive dysfunction [ 17 ]. The benefit of seahorses must have safety for human beings, one of which is through the serum biochemistry evaluation.…”

Section: Introductionmentioning

confidence: 99%

The seahorse (Hippocampus comes L.) extract ameliorates sperm qualities, testosterone level, and serum biochemistry in rats induced by depo medroxyprogesterone acetate

Mundijo¹,

Suyatna²,

Wibowo³

et al. 2023

J Adv Vet Anim Res

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Objective: The percentage of infertility cases in this world is about 50%. Seahorses ( Hippocampus spp.) are widely used in traditional medicine. Several studies suggest that seahorses have ethnopharmacological characteristics, such as fertility, antioxidants, and antifatigue. The purpose of this study was to determine whether seahorse extract (SE) ( Hippocampus comes L.) has an effect on fertility and serum biochemistry in rats induced by depo medroxyprogesterone acetate (DMPA). Materials and Methods: All animals were induced with 1.25 mg/kg BW of DMPA. Animals were grouped into five groups: namely aquadest, 1% CMC, and SE doses of 150, 225, and 300 mg/kg BW. The rats were gavage every morning from week 7 until 18. At the end of our study, semen from the vas deferens and blood from the heart were analyzed. We analyzed with a one-way analysis of variance and Bonferroni’s post hoc tests (α 95%). Results: The concentration of spermatozoa had a significant difference in dose of 150 mg/kg BW compared to other groups ( p = 0.04). In contrast, the motility ( p = 0.012) and viability of spermatozoa ( p = 0.007) were highly significant differences ( p < 0.05 and p < 0.01) at 300 mg/kg BW. Testosterone levels were not significantly ( p = 0.162; p > 0.05), but tended to increase at 300 mg/kg BW (11.01%). Nevertheless, serum biochemistry was insignificant ( p > 0.05) in all groups. Conclusion: SE ( Hippocampus comes L.) ameliorates fertility and serum biochemistry in rats induced by DMPA.

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“…Growing attention has been focused on discovering natural health products and derivatives of natural products from marine organisms due to their safety, high nutrition contents of trace elements and unique bioactive compounds that exhibit health beneficial effects. For example, some scientific evidence indicates the pharmacological properties of sea cucumbers, oysters, spoon/ sea worms, and sea horses can benefit human sexual and reproductive health (Matsuda and Walanabe, 2003;D'Aniello et al, 2012;Moghadam et al, 2016;Sudirman et al, 2021). These functions are summarized in Figure 1 and Table 1.…”

Section: Introductionmentioning

confidence: 99%

Bioactives From Marine Animals: Potential Benefits for Human Reproductive Health

et al. 2022

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Reproductive and sexual health issues, including infertility and sexual dysfunctions (SD), are common concerns affecting millions of reproductive age worldwide. Scattered literature reports that marine animals such as oysters, sea cucumbers, seahorses and spoon worms have unique bioactive compounds like saponins, steroids, seahorse-derived hydrolysates, polypeptide, oligopeptides and essential trace elements that significantly improve infertility, hormonal imbalance, SD, and impotence. In addition, these compounds have exhibited pharmacological properties against reproductive problems due to diabetes and exposure to electromagnetic fields, cyclophosphamide, or a high exercise load. This review presents the first critical assessment of the advances in understanding and applying bioactives from marine organisms to support human reproductive health. Key knowledge and technical gaps have been identified for future research to improve the lack of in-depth understanding of the mechanism and action of these bioactives in human clinical studies. There is a need to develop simple, selective, low-cost, and scalable processes to isolate and purify individual bioactive compounds for industrial applications. The optimizing culturing and farming conditions for specific bioactive compounds from targeted species are suggested for sustainable production. The review indicates a promising future of extracts and marine-derived bioactives as functional foods in preventing and managing human reproductive health issues, but mechanistic studies and further clinical trials are urgently required to evaluate their efficacy and safety.

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Hippocampus kuda protein hydrolysate improves male reproductive dysfunction in diabetic rats

Cited by 11 publications

References 48 publications

Seahorse Protein Hydrolysate Ameliorates Proinflammatory Mediators and Cartilage Degradation on Posttraumatic Osteoarthritis with an Obesity Rat Model

Seahorse Protein Hydrolysate Ameliorates Proinflammatory Mediators and Cartilage Degradation on Posttraumatic Osteoarthritis with an Obesity Rat Model

The seahorse (Hippocampus comes L.) extract ameliorates sperm qualities, testosterone level, and serum biochemistry in rats induced by depo medroxyprogesterone acetate

Bioactives From Marine Animals: Potential Benefits for Human Reproductive Health

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