His‐Tag‐Epitope Imprinted Thermoresponsive Magnetic Nanoparticles for Recognition and Separation Thyroid Peroxidase Antigens from Whole Blood Samples

Turan, Eylem

doi:10.1002/slct.201801557

Cited by 11 publications

(3 citation statements)

References 26 publications

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“…Tag for protein purification -His tag epitope on the surface of the magnetic nanoparticles -His-tag-epitope imprinted magnetic nanoparticles -Specific recognition and separation of thyroid peroxidase antigens [63] as Ni 2+ , that are coordinated to an EDTA moiety immobilized on a Fe3O4@SiO2 particle [45], or the FLAG-tag [46]. A further kind of directional immobilization relies on exploiting boronic acid moieties or its derivatives, as these would provide anchoring functions to fix the sugar moieties of glycoproteins, through reversible covalent bonds.…”

Section: His-tagmentioning

confidence: 99%

Molecularly imprinted polymers by epitope imprinting: a journey from molecular interactions to the available bioinformatics resources to scout for epitope templates

Pasquardini¹,

Bossi

2021

Anal Bioanal Chem

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The molecular imprinting of proteins is the process of forming biomimetics with entailed protein-recognition by means of a template-assisted synthesis. Protein-imprinted polymers (pMIPs) have been successfully employed in separations, assays, sensors, and imaging. From a technical point of view, imprinting a protein is both costly, for protein expression and purification, and challenging, for the preservation of the protein’s structural properties. In fact, the imprinting process needs to guarantee the preservation of the same protein three-dimensional conformation that later would be recognized. So far, the captivating idea to imprint just a portion of the protein, i.e., an epitope, instead of the whole, proved successful, offering reduced costs, compatibility with many synthetic conditions (solvents, pH, temperatures), and fine-tuning of the peptide sequence so to target specific physiological and functional conditions of the protein, such as post-translational modifications. Here, protein-protein interactions and the biochemical features of the epitopes are inspected, deriving lessons to prepare more effective pMIPs. Epitopes are categorized in linear or structured, immunogenic or not, located at the protein’s surface or buried in its core and the imprinting strategies are discussed. Moreover, attention is given to freely available online bioinformatics resources that might offer key tools to gain further rationale amid the selection process of suitable epitopes templates.

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Section: His-tagmentioning

confidence: 99%

Molecularly imprinted polymers by epitope imprinting: a journey from molecular interactions to the available bioinformatics resources to scout for epitope templates

Pasquardini¹,

Bossi

2021

Anal Bioanal Chem

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“…Epitope imprinting are introduced in detail in the above‐mentioned protein imprinting . Owing to amphiphilic and high molecular weight of polysaccharides, it is difficult to prepare MIPs by using the entire polysaccharides as a template.…”

Section: Biological Molecular Imprinting With Different Scalementioning

confidence: 99%

“…[168] "Epitope" Imprinting: Epitope imprinting are introduced in detail in the abovementioned protein imprinting. [169,170] Owing to amphiphilic and high molecular weight of poly saccharides, it is difficult to prepare MIPs by using the entire poly saccharides as a template. Therefore, similar method, "epitope" imprinting, was introduced into polysaccharides imprinting.…”

Section: Polysaccharides Imprintingmentioning

confidence: 99%

Molecularly Imprinted Materials for Selective Biological Recognition

Zhang

et al. 2019

Macromol. Rapid Commun.

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Molecular imprinting is an approach of generating imprinting cavities in polymer structures that are compatible with the target molecules. The cavities have memory for shape and chemical recognition, similar to the recognition mechanism of antigen–antibody in organisms. Their structures are also called biomimetic receptors or synthetic receptors. Owing to the excellent selectivity and unique structural predictability of molecularly imprinted materials (MIMs), practical MIMs have become a rapidly evolving research area providing key factors for understanding separation, recognition, and regenerative properties toward biological small molecules to biomacromolecules, even cell and microorganism. In this review, the characteristics, morphologies, and applicability of currently popular carrier materials for molecular imprinting, especially the fundamental role of hydrogels, porous materials, hierarchical nanoparticles, and 2D materials in the separation and recognition of biological templates are discussed. Moreover, through a series of case studies, emphasis is given on introducing imprinting strategies for biological templates with different molecular scales. In particular, the differences and connections between small molecular imprinting (bulk imprinting, “dummy” template imprinting, etc.), large molecular imprinting (surface imprinting, interfacial imprinting, etc.), and cell imprinting strategies are demonstrated in detail. Finally, future research directions are provided.

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A fluorescent artificial receptor with specific imprinted cavities to selectively detect colistin

Turan

Zengi̇n

2020

Anal Bioanal Chem

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His‐Tag‐Epitope Imprinted Thermoresponsive Magnetic Nanoparticles for Recognition and Separation Thyroid Peroxidase Antigens from Whole Blood Samples

Cited by 11 publications

References 26 publications

Molecularly imprinted polymers by epitope imprinting: a journey from molecular interactions to the available bioinformatics resources to scout for epitope templates

Molecularly imprinted polymers by epitope imprinting: a journey from molecular interactions to the available bioinformatics resources to scout for epitope templates

Molecularly Imprinted Materials for Selective Biological Recognition

A fluorescent artificial receptor with specific imprinted cavities to selectively detect colistin

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