2008
DOI: 10.1080/14756360701841913
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His22 of TLXI plays a critical role in the inhibition of glycoside hydrolase family 11 xylanases

Abstract: Recently, a novel wheat thaumatin-like protein, TLXI, which inhibits microbial glycoside hydrolase family (GH) 11 xylanases has been identified. It is the first xylanase inhibitor that exerts its inhibition in a non-competitive way. In the present study we gained insight into the interaction between TLXI and xylanases via combined molecular modeling and mutagenic approaches. More specifically, site-specific mutation of His22, situated on a loop which distinguishes TLXI from other, non-inhibiting, thaumatin-lik… Show more

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Cited by 10 publications
(11 citation statements)
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“…The circular dichroism (CD) spectrum of TLXI (Figure 3) confirmed the absence of a-helices in the TLXI structure, much as noted earlier [23] for its recombinant counterpart as the characteristic dual minimum at 208 and 222 nm for a-helices was not seen. The spectrum shows a single minimum between 210 and 215 nm and an increase at 200 nm, typical for antiparallel b-sheets.…”
Section: Structural Features Of Tlxisupporting
confidence: 51%
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“…The circular dichroism (CD) spectrum of TLXI (Figure 3) confirmed the absence of a-helices in the TLXI structure, much as noted earlier [23] for its recombinant counterpart as the characteristic dual minimum at 208 and 222 nm for a-helices was not seen. The spectrum shows a single minimum between 210 and 215 nm and an increase at 200 nm, typical for antiparallel b-sheets.…”
Section: Structural Features Of Tlxisupporting
confidence: 51%
“…The 3D structural model of TLXI [23] shows that it consists of mainly of b-strands and that the a-helices, present in the thaumatin structure [26], are absent in TLXI (Figure 1). The alignment of the amino acid sequence of TLXI with that of thaumatin shows a deletion of 50 amino acids (Figure 2), corresponding to domain II of thaumatin which contains the ahelices.…”
Section: Structural Features Of Tlximentioning
confidence: 99%
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“…The inactive H22A TLXI mutant from T. aestivum was expressed and purified as described previously 15. The purified TLXI mutant was concentrated to 3.3 mg/mL in 25 m M sodium acetate (pH 5.0).…”
Section: Methodsmentioning
confidence: 99%
“…Mutagenesis studies of TLXI have pointed to the histidine at position 22 as being important for inhibition of xylanases since mutation of this residue to alanine abolished the inhibition activity of TLXI 15…”
Section: Introductionmentioning
confidence: 99%