Histamine downregulates monocyte CCL2 production through the histamine H4 receptor

Dijkstra, Dorothea; Leurs, Rob; Chazot, Paul L.; Shenton, Fiona C.; Stark, Holger; Werfel, Thomas; Gutzmer, Ralf

doi:10.1016/j.jaci.2007.03.024

Cited by 122 publications

(126 citation statements)

References 32 publications

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“…Separation of human PBMCs was performed using density centrifugation with Lymphoprep (Fresenius Kabi Norge AS, Oslo, Norway) (Dijkstra et al, 2007). Isolation was carried out under sterile conditions to prevent monocyte activation.…”

Section: Pbmc Isolationmentioning

confidence: 99%

Quercetin ameliorates LPS-induced inflammation in human peripheral blood mononuclear cells by inhibition of the TLR2-NF-κB pathway

Zhang

Lin

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Quercetin, a dietary flavonoid abundant in fruits, vegetables, and herbs, presents various pharmacological effects. This study aimed to investigate the anti-inflammatory effect and the underlying mechanism of quercetin in lipopolysaccharide (LPS)-stimulated human peripheral blood mononuclear cells (PBMCs). Cell viability was measured by the Cell Counting Kit-8 assay. The mRNA expression of Toll-like receptor 2 (TLR2) was assessed by quantitative real-time polymerase chain reaction. Inflammatory cytokine secretions and nuclear factor (NF)-kB levels were analyzed by enzyme-linked immunosorbent assay. Our findings showed that quercetin significantly reduced LPS-induced cytotoxicity in human PBMCs. Quercetin suppressed the secretion of tumor necrosis factor-a, interleukin (IL)-1b, and IL-6 in LPS-stimulated human PBMCs. Moreover, quercetin reduced the LPS-induced increase in the expression of TLR2 mRNA and decreased the NF-kB concentration in LPS-stimulated human PBMCs. The data indicates that quercetin plays an important role in

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Section: Pbmc Isolationmentioning

confidence: 99%

Quercetin ameliorates LPS-induced inflammation in human peripheral blood mononuclear cells by inhibition of the TLR2-NF-κB pathway

Zhang

Lin

et al. 2016

Genet. Mol. Res.

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“…The final concentration of the organic solvent was less than 0.1%, which had no effect on cell viability. Antibodies H 3 R and H 4 R were detected by using the anti-hH 3 R (349-358) and the anti-hH 4 R (374-390) antibodies produced and validated for detecting both human and rodent H 3 R and H 4 R in the School of Biological and Biomedical Sciences, Durham University [21][22][23][24][25][26][27]. Briefly, anti-hH 3 R antibody was generated coupling the peptide corresponding to the amino acids RLSRDRKVAK Cys of the human and rat H 3 R to thyroglobulin by m-maleimidobenzoic acid N-hydroxysuccinimide ester coupling method as described previously [6] and antibodies were raised in rabbits to the resultant conjugate.…”

Section: Reagentsmentioning

confidence: 99%

Histamine receptor expression in human renal tubules: a comparative pharmacological evaluation

et al. 2015

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Objective and design The aim of this study is to evaluate the expression of the histamine receptors, particularly focusing on the H 4 R in human renal tubules. MaterialThe ex-vivo evaluation was carried on specimens from human renal cortex. Primary and immortalized tubular epithelial cells (TECs) and the HK-2 cell line were used as in vitro models.Treatment Cells were pretreated for 10 min with chlorfeniramine maleate 10 µM (H 1 R antagonist), ranitidine 10 µM (H 2 R antagonist), GSK189254 1 µM (H 3 R antagonist) or JNJ7777120 10 µM (H 4 R antagonist), and then exposed to histamine (3 pM -10 nM) for 30 min. MethodsThe ex-vivo evaluation on specimens from human renal cortex was performed by immunohistochemistry. The expression of histamine receptors on primary and immortalized TECs and the HK-2 cell line was evaluated at both gene (RT-PCR) and protein (immunocytofluorescence) levels. The pharmacological analysis was performed by TR-FRET measurements of second messenger (IP3 and cAMP) production induced by histamine with or without the selective antagonists.Results Our data revealed the presence of all histamine receptors in human tubules; however, only TECs expressed all the receptors. Indeed, histamine elicited a sigmoid dose-response curve for IP 3 production, shifted to the right by chlorpheniramine maleate, and elicited a double bell-shaped curve for cAMP production, partially suppressed by the selective H 2 R, H 3 R and H 4 R antagonists when each added alone, and completely ablated when combined together.Conclusions Herein, we report the identification of all four histamine receptors in human renal tubules.

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“…The characterization of the H4 as the immune system histamine receptor directed growing attention toward its therapeutic exploitation in chronic inflammatory disorders, such as allergy, asthma (Dunford et al, 2006), chronic pruritus (Dunford et al, 2007;Dijkstra et al, 2007;Bäumer et al, 2008), inflammatory bowel disease (Varga et al, 2005;Dunford et al, 2007), and autoimmune diseases, such as rheumatoid arthritis (Ikawa et al, 2005) and multiple sclerosis (JadidiNiaragh and Mirshafiey, 2010).…”

Section: Introductionmentioning

confidence: 99%

Pleiotropic effect of histamine H4 receptor modulation in the central nervous system

2013

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a b s t r a c tThe histamine H4 receptor (H4R) is expressed primarily on cells involved in inflammation and immune responses. Recently, it has been reported the functional expression of H4R within neurons of the central nervous system, but their role has been poorly understood. The present study aimed to elucidate the physiopathological role of cerebral H4R in animal models by the intracerebroventricular administration of the H4R agonist VUF 8430 (20e40 mg per mouse). Selectivity of results was confirmed by the prevention of the effects produced by the H4R antagonist JNJ 10191584 (3e9 mg/kg p.o.). Neuronal H4R activation induced acute thermal antinociception, indicating that neuronal histamine H4R might be involved in the production of antinociception in the absence of an inflammatory process. An anxiolyticlike effect of intensity comparable to that exerted by diazepam, used as reference drug, was produced in the lightedark box test. VUF 8430 reversed the scopolamine-induced amnesia in the passive avoidance test and showed anorexant activity in food deprived mice. Conversely, the H4R activation did not modify the immobility time in the tail suspension test. Rotarod performance test was employed to demonstrate that the effects observed following the administration of VUF 8430 and JNJ 10191584 were not due to impaired motor function of animals. Furthermore, both compounds did not alter spontaneous mobility and exploratory activity in the hole board test. These results show the antinociceptive, antiamnesic, anxiolytic and anorexant effects induced by neuronal H4R agonism, suggesting that H4 modulators may have broader utility further the control of inflammatory and immune processes.

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Histamine downregulates monocyte CCL2 production through the histamine H4 receptor

Cited by 122 publications

References 32 publications

Quercetin ameliorates LPS-induced inflammation in human peripheral blood mononuclear cells by inhibition of the TLR2-NF-κB pathway

Quercetin ameliorates LPS-induced inflammation in human peripheral blood mononuclear cells by inhibition of the TLR2-NF-κB pathway

Histamine receptor expression in human renal tubules: a comparative pharmacological evaluation

Pleiotropic effect of histamine H4 receptor modulation in the central nervous system

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