Histamine release induced by glucose (mannose)-specific lectins isolated from Brazilian beans. Comparison with concanavalin A

Abstract

The histamine releasing properties of glucose (mannose)-specific lectins isolated from Brazilian beans was examined. The Canavalia brasiliensis, Dioclea rostrata, and Dioclea virgata lectins induced histamine release in rat peritoneal mast cells similar to concanavalin A. Less potency and efficacy was observed for Canavalia maritima, Dioclea guianensis, and Dioclea violacea while very low activities were seen for the lectins from Dioclea grandiflora, Canavalia bonariensis, and Cratylia floribunda. The histamine releasing effect was quenched by higher doses of D. virgata lectin similar to what was reported for concanavalin A. This effect was abrogated by increasing the concentration of calcium in the incubating medium. As these above proteins have sites that bind calcium, higher doses of the lectins might withdraw the calcium which is essential for the mast cell secretion.

Thermodynamic Binding Studies of Lectins from the Diocleinae Subtribe to Deoxy Analogs of the Core Trimannoside of Asparagine-linked Oligosaccharides

“…However, despite the phylogenetic proximity and apparently highly conserved sequences of the above Dioclineae lectins, they possess different biological activities such as histamine release from rat peritoneal mast cells (19), lymphocyte proliferation and interferon ␥ production (20), peritoneal macrophage stimulation and inflammatory reaction (21) as well as induction of paw edema and peritoneal cell immigration in rats (22). Indeed, the importance of determining the fine binding Fig.…”

Thermodynamics of Binding of the Core Trimannoside of Asparagine-linked Carbohydrates and Deoxy Analogs to Dioclea grandiflora Lectin

“…1) (3). Furthermore, both ConA and DGL possess different biological activities such as histamine release from rat peritoneal mast cells (4). Thus, it is important to investigate the detailed carbohydrate binding specificity of DGL in order to understand the molecular basis for its biological activities relative to other members of the Diocleinae subtribe including ConA.…”

“…In order to gain insight into the detailed binding specificities of DGL and ConA, a series of mono and dideoxy derivatives (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14) of methyl 3,6-di-O-(␣-mannopyranosyl)-␣-mannopyranoside (1) (Fig. 1) were synthesized and tested for their ability to bind to both lectins as determined by hemagglutination inhibition methods (3,5).…”

Molecular Cloning and Characterization of ConBr, the Lectin of Canavalia Brasiliensis Seeds

“…Differences in the histamine release by rat peritoneal mast cells induced by injection of glucose/mannose-specific lectins isolated from the beans of the plants C. brasiliensis, Diocleu rostrutu, Dioclea virgata, Cunavalia maritima, Dioclea guianensis, Dioclea violacea, Dioclea grandiflora, Canavalia bonuriensis, Cratyliu foribunda, and Canavalia ensijormis, which belong to the same tribe and sub-tribe (Phaseoleae and Diocleinae, respectively), have been reported (Gomes et al, 1994). In addition, these lectins display significant variation in their potency of stimulation of human lymphocyte proliferation and interferon y production by peripheral blood mononuclear cells (Barral-Netto et al, 1992).…”