Histamine Release Inhibitory Activity of Piper nigrum Leaf

Abstract

During the course of our search for useful ingredients of cosmetics from natural resource, we have previously reported that Piper nigrum leaf extract showed both melanogenesis stimulation activity and testosterone 5a-reductase inhibitory activity and that (Ϫ)-cubebin (1) and (Ϫ)-3,4-dimethoxy-3,4-desmethylenedioxycubebin (2) were isolated as active lignan constituents. [1][2][3][4] It was suggested that these substances may be useful ingredients of hair-care cosmetics for prevention of gray hair and alopecia. As a part of our continuous study on P. nigrum leaf, we examined anti-allergic effect of the leaf, because hair care cosmetics having anti-allergic activity may be desirable. It has been reported that antiallergic effect of a botanical formulation 5) in which P. nigrum fruit was included. Anti-allergic activity of piperine, 6) a major alkaloid amide of P. nigrum fruit have been reported, but HPLC analysis revealed that the piperine content in the leaf was 0.00007%. 7) Moreover, we have not found any reports on anti-allergic effect of the leaf. In this paper, we describe in vivo anti-allergic effects of P. nigrum leaf extract against type I allergy model and type IV allergy model in mice. 2,4-Dinitrofluorobenzene (DNFB) has been shown to induce triphasic increase in ear thickness of mouse by the challenge after a passive sensitization with monoclonal antidinitrophenyl (DNP) IgE antibody. 8,9) This model serves as an animal model for type I allergy. 8,9) In addition, we used picryl chloride (PC)-induced contact dermatitis in mice as an animal model for type IV allergy.10) Inhibitory effects of Piper nigrum leaf extract, on histamine release were also examined by in vitro assay using compound 48/80-induced histamine release from rat peritoneal mast cells. MATERIALS AND METHODS Plant MaterialsPiper nigrum leaf was the same sample described in the previous paper. 1-4)Reagents 2,4-Dinitrofluorobenzene (DNFB), Ficoll, Hanks' balanced salt solution, olive oil, o-phthaldialdehyde, picryl chloride (PC), and sodium carboxymethyl cellulose (CMC · Na) were purchased from Nacalai Tesque (Tokyo, Japan). Sodium cromoglycate (SCG) was purchased from Funakoshi (Tokyo, Japan). Compound 48/80, dimethyl sodium salt (DMSO), prednisolone, monoclonal anti-DNP antibody and heparin sodium salt were purchased from Sigma (St. Louis, MO, U.S.A.). Other chemical reagents were reagent grade and were purchased from Wako Pure Chemical Industries, Ltd. unless otherwise noted.Preparation of PN-ext and Fractionation Methanolic extract of Piper nigrum leaf (PN-ext) was obtained according to the previous paper in yield of 14.0%. The powdered dry leaves (500 g) of P. nigrum were extracted with MeOH (5 lϫ3 times) for 1 h under reflux. Combined extracts were evaporated under reduced pressure to give a methanolic extract (PN-ext, 71.1 g). A part of PN-ext (69.6 g) was extracted with hexane (400 mlϫ3), and the organic layer was evaporated to give a hexane soluble fraction (17.3 g). The hexane insoluble part was extracted with EtOAc (300 mlϫ2). The E...

Effects of dihydrocubebin, a lignan isolated from Indonesian plant Piper cubeba, on the histamine release from rat mast cells

“…Ethanolic extract of Piper betle leaves decreased histamine and GM-CSF produced by an IgE-mediated hypersensitive reaction, and inhibited eotoxin and IL-8 secretion as well in a TNF-α and IL-4-induced allergic reaction (Wirotesangthong et al, 2007). While methanolic extract of Piper nigrum leaves inhibited histamine release from rat peritoneal mast cell, a powerful inhibition on DNFB-induced cutaneous reaction at 1 h and 24 h after DNFB challenge in mice, which passively sensitized with anti-DNP IgE antibody upon oral administration of this extract (Hirata et al, 2008).…”

Piper nigrum Fruit Extract Prevents TMA-Induced Allergic Contact Dermatitis by Regulating Th2 Cytokine Production

“…Additionally, HPLC-MS approaches have identified several compounds from Piper nigrum including pellitorine, piperylin, 4,5-dihydropiperlonguminine, piperlonguminine, 4,5-dihydropiperine, piperine, and pipercide (Scott et al, 2005). A previous study has shown that oral administration of a methanolic extract of Piper nigrum leaf suppresses dinitrofluorobenzene (DNFB)-induced immune reactions in vivo and histamine release by rat peritoneal mast cells (Hirata et al, 2008). However, the effect of Piper nigrum fruit extract (PFE) on hapten-induced dermatitis and its mechanism of action have not been explored in detail.…”

Antiallergic activity of unripe Citrus hassaku fruits extract and its flavanone glycosides on chemical substance-induced dermatitis in mice

“…The reaction was quenched by cooling the mixture on ice. The mixture was centrifuged at 150 g and 5°C for 5 min, then histamine in the supernatant fluid was assayed fluorometrically according to the method described in the previous report [7]. The effect of the test substance on histamine release from mast cells induced by compound 48/80 was expressed as histamine release percentage.…”

“…Thus, since the unripe fruit of C. hassaku collected by thinning out can be considered a plant resource, we examined in vivo antiallergic effects of CH-ext and its two major flavanone glycosides, naringin and neohesperidin, against type I and type IV allergy models in mice. 2,4-Dinitrofluorobenzene (DNFB) has been shown to induce triphasic increase in ear thickness in mice after a passive sensitization with monoclonal anti-dinitrophenyl (DNP) IgE antibody [6,7]. This method serves as an animal model for type I allergic reaction [6,7].…”

“…2,4-Dinitrofluorobenzene (DNFB) has been shown to induce triphasic increase in ear thickness in mice after a passive sensitization with monoclonal anti-dinitrophenyl (DNP) IgE antibody [6,7]. This method serves as an animal model for type I allergic reaction [6,7]. As type IV allergic reaction model, we used picryl chloride-induced contact dermatitis (PC-CD) in mice [8], as applied by others [9].…”