Histamine secretion from human skin slices induced by anti‐IgE and artificial secretagogues and the effects of sodium cromoglycate and salbutamol

Abstract

Human cutaneous mast cells show functional differences from their counterparts in other tissues. Following passive sensitization with T^ atopic serum for 30 min at 37 C human skin slices released histamine after challenge with anti-human IgE in a concentration dependent manner. Maximum release of 14 + 2";, was achieved with a 1/10 dilution of anti-IgE. Passive sensiti/ation with 10% atopic serum increased the secretory response to anti-IgE but histamine release was only concentration related over the entire l/IOOO to 1/10 dilution range in half of the specimens studied, the remainder showing high dose tolerance to anti-IgE, Negligible histamine release occurred with anti-IgE challenge of slices which had not been passively sensitized.The histamine releasing ability of A23187 in human skin slices was similar to that observed in lung and adenoidal mast cells being concentration dependent over the range 0-1-3 fiM with a maximum release of 25±3%. In contrast to human lung and adenoidal mast cells. poly-L-lysine and compound 48/80 induced histamine release from skin slices. Poly-L-lysine induced a concentration-dependent release ofhistamine over the range O-Ol-IO f.tM with a maximum of 27 + 3%. The response to compound 48/80 was variable, releasing in some but not all specimens, Histamine release caused by anti-IgE. A23I87 and poly-L-lysine was shown to be dependent upon extracellular calcium while release stimulated by compound 48/80 was calcium independent. The chemotactic peptide. formyl-methionyl-leucyl-phenylalanine, over the range O'Oi-10 /(M failed to release histamine from skin slices. Sodium cromoglycate (100 1000 /(M) failed to inhibit histamine release and the ^-adrenoceptor stimulant saibutamol (I-IO fM) showed only weak activity at the lowest of three different concentrations of anti-IgE used for challenge.

Inhibition of IgE‐dependent histamine release from human dispersed lung mast cells by anti‐allergic drugs and salbutamol

“…Histamine releasing cells of man, like those of the rat, show heterogeneity with respect to the action of SCG. Human basophil leucocytes (Church, 1982) and skin mast cells (Ting et al, 1983;Clegg et al, 1985) are refractory to its inhibitory effects. Immunological histamine release from human lung fragments is partially inhibited by SCG (Butchers et al, 1979;, but the variability of this model makes quantification of responses difficult .…”

Comparison of the Modulatory Effect of Ketotifen, Sodium Cromoglycate, Procaterol and Salbutamol in Human Skin, Lung and Tonsil Mast Cells

“…It has already been demonstrated that the responsiveness of IgE-mediated histamine release to modulation by sodium cromoglycate (SCG) differs markedly between human skin and lung mast cells [4], In mast cells dispersed from human lung tissue [6][7][8][9] and adenoids [10], SCG is a weak inhib itor of histamine and prostaglandin (PG) D2 release. On the other hand, in skin mast cells SCG has no inhibitory ef fect on IgE-dependent histamine release in in vitro [4,11,12] and in vivo studies [13][14][15].…”

Efficacy and acceptability of a new topical skin lotion of sodium cromoglicate (Altoderm) in atopic dermatitis in children aged 2-12 years: a double-blind, randomized, placebo-controlled trial

“…However, experiments on human skin are conflicting. Three centres have shown that the drug does not prevent the release of histamine or other mediators from human skin, 23 from human skin slices 24 or from dispersed skin mast cells 25 . However, it has been shown to prevent the release of the inflammatory cytokine, tumour necrosis factor‐α, from mast cells in human skin 26 .…”