Histamine stimulates secretion of extracellular vesicles with nucleotidase activity in rat submandibular gland

González, Débora A.; Haaster, Martín Matías Barbieri van; Villarruel, Emmanuel Quinteros; Brandt, Macarena; Benitez, María Belén; Stranieri, Graciela; Orman, Betina

doi:10.1016/j.archoralbio.2017.10.010

Cited by 7 publications

(5 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Discussioncontrasting

confidence: 99%

“…Contrary to the findings of this work, demonstrating that EVP secretion is not affected by histamine, two previously published studies , have reported an increase in EVP secretion upon histamine stimulation in non-gastric cells. Gonzalez et al, who stimulated rat submandibular glands with 10 nM histamine for 30 min, estimated the EVP amount using their protein content and nucleotidase activity, which are bulk methods that do not quantify individual particles. Verweij et al found an increase in fusion events of MVBs with the plasma membrane, resembling the secretion of only one class of EVs, namely, exosomes, within 1–5 min after activation with 100 μM histamine in HeLa and HUVEC cells.…”

Section: Discussioncontrasting

confidence: 99%

See 1 more Smart Citation

Extracellular Vesicles and Particles Modulate Proton Secretion in a Model of Human Parietal Cells

et al. 2023

View full text Add to dashboard Cite

The secretion of extracellular vesicles and particles (EVPs) is an important mechanism of cellular communication. In this work, we demonstrate a functional role of EVPs in mechanisms regulating gastric acid secretion. HGT-1 cells were used as a model system to assess proton secretion. First, in order to prove EVP secretion by HGT-1 cells, EVPs were isolated by size exclusion chromatography and characterized by nanoparticle tracking analysis, Western blot, and cryo transmission electron microscopy. For examination of the potential role of EVPs in proton secretion, HGT-1 cells were treated with pharmacological EVinhibitors, resulting in a reduction of histamine-induced proton secretion. To demonstrate the functional role of EVPs in the mechanism of proton secretion, EVP-conditioned supernatant was collected after stimulation of HGT-1 cells with histamine, fractionated, and subjected to an activity screening. The results revealed constituents of the HGT-1-derived secretome with an MW of >100 kDa (including EVPs) to modulate proton secretion, while smaller constituents had no effect. Finally, a dose-dependent modulatory effect on proton secretion of HGT-1 cells was demonstrated by isolated HGT-1-derived EVPs. Hence, this study presents first results on the potential function of EVPs as a previously undiscovered mechanism of regulation of gastric acid secretion by parietal cells.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 99%

Extracellular Vesicles and Particles Modulate Proton Secretion in a Model of Human Parietal Cells

et al. 2023

View full text Add to dashboard Cite

show abstract

“…In the AR mice, local swelling of nasal mucosae was observed, which may have been caused by histamine- and kinin-induced angiotelectasis and the subsequent enhancement of vascular wall permeability by stimulation of the receptors located on sensory nerves and blood vessel ( 13 ). Histamine is also known to augment glandular secretion and plasma effusion ( 13 ). Plasma effusion may have resulted in the nasal mucosal edema and swelling ( 14 ).…”

Section: Discussionmentioning

confidence: 99%

C‑C chemokine receptor type 3 gene knockout alleviates inflammatory responses in allergic rhinitis model mice by regulating the expression of eosinophil granule proteins and immune factors

et al. 2018

View full text Add to dashboard Cite

The present study aimed to investigate the effects of C-C chemokine receptor type 3 (CCR3) gene knockout on allergic rhinitis (AR) in mice, as well as the underlying molecular mechanisms. Ovalbumin was administrated to CCR3+/+ and CCR3−/− BALB/c mice to establish an AR model. The mice were divided into four groups: i) Normal control (CG), ii) AR model (AR), iii) CCR3 knockout CG (CCR3−/−CG) and iv) AR model with CCR3 knockout (CCR3−/−AR). Histological sections of nasal mucosae were examined by hematoxylin and eosin staining, which revealed that CCR3 knockout suppressed the invasion of inflammatory cells and relieved the damage of nasal mucosae. Peripheral blood smear and nasal-washing smears were evaluated by Wright's staining. Eosinophil (EOS) numbers in nasal mucosae, peripheral blood, and nasal washings of the various groups were ranked in the order: AR>CCR3−/−AR>CG>CCR3−/−. mRNA expression levels of CCR3, EOS peroxidase (EPO), EOS cationic protein (ECP), and major basic protein (MBP) in the peripheral serum and nasal washings were detected by reverse transcription-polymerase chain reaction. Interferon-γ (IFN-γ), interleukin (IL)-4, IL-10, and immunoglobulin E (IgE) protein levels in the peripheral serum and nasal washings were investigated by ELISA. CCR3 mRNA expression was not detected in the CCR3−/− and CCR3−/−AR groups, whereas expression levels in the AR group were markedly higher compared with expression in the CG group. Compared with the CG-associated groups (i.e., the CG and CCR3−/−CG groups), the levels of EPO, ECP, MBP, IL-4, and IgE were significantly increased in the AR-associated groups (that is, R and CCR3−/−AR). In addition, the CCR3−/−AR group mice produced significantly lower levels of EPO, ECP, MBP, IL-4 and IgE compared with the AR group, whereas the expression levels of IFN-γ and IL-10 were increased. CCR3 gene knockout may alleviate EOS invasion and the inflammatory response in AR model mice by reducing the expression levels of EPO, ECP, MBP, IL-4, and IgE, and increasing the expression of IL-10 and IFN-γ.

show abstract

“…In line with these findings, Gonzales and coll. [33] have also shown that histamine, which is present in pro-inflammatory environment such as that of periodontal disease, can stimulate the secretion of EVs with ecto-nucleotidase activity from the rat submandibular gland. In this way, histamine, by activating its H 4 receptors, may contribute toward regulating the pro-inflammatory eATP levels, favoring the formation of tissue-protecting ADO.…”

Section: Purines and Evs Derived From Normal Or Pathological Non-cancer Cells/tissuesmentioning

confidence: 95%

Adenine-Based Purines and Related Metabolizing Enzymes: Evidence for Their Impact on Tumor Extracellular Vesicle Activities

Iorio

Ciccarelli

2021

Cells

View full text Add to dashboard Cite

Extracellular vesicles (EVs), mainly classified as small and large EVs according to their size/origin, contribute as multi-signal messengers to intercellular communications in normal/pathological conditions. EVs are now recognized as critical players in cancer processes by promoting transformation, growth, invasion, and drug-resistance of tumor cells thanks to the release of molecules contained inside them (i.e., nucleic acids, lipids and proteins) into the tumor microenvironment (TME). Interestingly, secretion from donor cells and/or uptake of EVs/their content by recipient cells are regulated by extracellular signals present in TME. Among those able to modulate the EV-tumor crosstalk, purines, mainly the adenine-based ones, could be included. Indeed, TME is characterized by high levels of ATP/adenosine and by the presence of enzymes deputed to their turnover. Moreover, ATP/adenosine, interacting with their own receptors, can affect both host and tumor responses. However, studies on whether/how the purinergic system behaves as a modulator of EV biogenesis, release and functions in cancer are still poor. Thus, this review is aimed at collecting data so far obtained to stimulate further research in this regard. Hopefully, new findings on the impact of adenine purines/related enzymes on EV functions may be exploited in tumor management uncovering novel tumor biomarkers and/or druggable targets.

show abstract

Histamine stimulates secretion of extracellular vesicles with nucleotidase activity in rat submandibular gland

Cited by 7 publications

References 33 publications

Extracellular Vesicles and Particles Modulate Proton Secretion in a Model of Human Parietal Cells

Extracellular Vesicles and Particles Modulate Proton Secretion in a Model of Human Parietal Cells

C‑C chemokine receptor type 3 gene knockout alleviates inflammatory responses in allergic rhinitis model mice by regulating the expression of eosinophil granule proteins and immune factors

Adenine-Based Purines and Related Metabolizing Enzymes: Evidence for Their Impact on Tumor Extracellular Vesicle Activities

Contact Info

Product

Resources

About