Histamine suppresses neopterin production in the human myelomonocytoma cell line THP-1

Gruber, Alexandra Carina; Murr, Christian; Wirleitner, Barbara; Werner-Felmayer, Gabriele; Fuchs, Dietmar

doi:10.1016/s0165-2478(00)00171-1

Cited by 12 publications

(9 citation statements)

References 24 publications

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“…Results showed that various dietary supplements as well as pharmacologic agents significantly inhibited lipopolysaccharide-stimulated TNF-␣ release (1 ). Interestingly, this assay matches almost perfectly one we proposed that also uses THP-1 cells but uses neopterin production as a read-out for monitoring potential antiinflammatory effects of compounds (2 ). After publication of that report, we observed that drugs usually exert more important effects on the T-cell/macrophage interplay than on the stimulated monocytic cells themselves and thereby on the Th1-type cytokine interferon-␥, which is crucially important as a proinflammatory mediator.…”

Section: In Vitro Testing For Antiinflammatory Properties Of Compoundssupporting

confidence: 67%

In Vitro Testing for Antiinflammatory Properties of Compounds

2006

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Section: In Vitro Testing For Antiinflammatory Properties Of Compoundssupporting

confidence: 67%

In Vitro Testing for Antiinflammatory Properties of Compounds

2006

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“…The effects of antioxidant compounds were comparable to those observed earlier when effects of the Th2-type cytokine IL-4 or allergic mediators like histamine were investigated on stimulated PBMC and monocytic THP-1 cells [65,73], and they were also evident when the expression of NF-ĸB was monitored [55]. Overall, a close relationship was demonstrated between the stimulated expression of NF-ĸB and the production of neopterin and degradation of tryptophan [67], and compounds like phenols, flavonoids and ascorbic acid exert a suppressive influence as chemical antioxidants and may detoxify ROS.…”

Section: Cross-regulation Of Th1- and Th2-type Immune Responsessupporting

confidence: 65%

Potential Role of Antioxidant Food Supplements, Preservatives and Colorants in the Pathogenesis of Allergy and Asthma

Zaknun

Schroecksnadel²,

Kurz

et al. 2011

Int Arch Allergy Immunol

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A significant increase in the incidence of allergy and asthma has been observed during the past decades. The background of this phenomenon has not been well explained, but changes in lifestyle and habits are heavily discussed as contributing factors. Among these is a too clean environment, which may predispose individuals to increased sensitivity to allergic responses. Also the increase in dietary supplements including preservatives and colorants may contribute to this. In vitro, we and others have shown in freshly isolated human peripheral blood mononuclear cells that antioxidant compounds like vitamins C and E as well as food preservatives and colorants exert significant suppressive effects on the Th1 immune activation cascade. The effects observed may be based on the interaction of antioxidant compounds with proinflammatory cascades involving important signal transduction elements such as nuclear factor-ĸB. Although only obtained in vitro, these results show an anti-inflammatory property of compounds which could shift the Th1-Th2-type immune balance towards Th2-type immunity. This review article discusses the potential role of increased use of antioxidant food supplements as well as preservatives and colorants in the increase in allergy and asthma in the Western world.

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“…Ledochowski et al investigated 709 healthy outpatients and found that increased Ig-E concentrations, as in allergic disease, were associated with low neopterin values and verse vica [25]. In an experimental setting Gruber et al observed, that histamine, an important inflammatory mediator in allergic disease, decreased neopterin formation in mononuclear cells in a dose dependent manner [26].…”

Section: Discussionmentioning

confidence: 99%