Histidase function in human epidermal cells

Barnhisel, Mary L.; Priest, Robert E.; Priest, Jean H.

doi:10.1002/jcp.1040760105

Cited by 11 publications

(2 citation statements)

References 21 publications

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“…The enzyme histidase found in normal epidermis enhances cellular concentration of histidine that can be used for stimulated synthesis of histidine-rich proteins involved in the keratin formation during tumorigenesis (45). Histidase activity in skin carcinoma bearing mouse has been found to be absent and lowered in TPA-treated mice (45,46). Animals showing squamous cell carcinoma following AO/TPA or SANG/TPA administration caused inhibition of histidase activity, which was found to be enhanced following topical application of TOCO and NAC, implicating the decreased synthesis of keratin as observed in histopathological studies.…”

Section: Downloaded By [Umeå University Library] At 03:27 06 October mentioning

confidence: 87%

Protective Effect of Topical Application of α-Tocopherol and/or N-Acetyl Cysteine on Argemone Oil/Alkaloid-Induced Skin Tumorigenesis in Mice

Pal

Alam

Singhal

et al. 2013

Nutrition and Cancer

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Since bioantioxidants in plasma of Epidemic Dropsy patients [a condition caused by consumption of adulterated mustard oil with argemone oil (AO)] were found to be significantly decreased, the beneficial effect of N-acetyl cysteine (NAC) and α-tocopherol (TOCO) against AO- or sanguinarine (SANG)-induced tumorigenicity was undertaken in mice. Topical application of TOCO and NAC either alone or in combination showed significant protection against AO/TPA- and SANG/TPA-induced skin tumorigenicity. Histopathological findings suggest that papillomatous growth in AO/TPA- and SANG/TPA-treated animals were substantially protected following topical application of TOCO or NAC. Further, treatment of TOCO and NAC either alone or in combination to AO/TPA- or SANG/TPA-induced mice significantly decreased lipid peroxidation, along with significant revival in glutathione (GSH) content and activities of tyrosinase, histidase, catalase, SOD, GSH peroxidase, and GSH reductase in skin. In vitro studies showed that TOCO and/or NAC significantly decreased the AO and SANG induced cell proliferation and activation of ERK, p38, JNK MAPKs and NF-κB signaling in HaCaT cells. In summary, TOCO and NAC may be useful in preventing the tumorigenic response of AO and SANG probably by acting as scavenger of free radicals and inhibiting MAPKs and NF-κB signaling.

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Section: Downloaded By [Umeå University Library] At 03:27 06 October mentioning

confidence: 87%

Protective Effect of Topical Application of α-Tocopherol and/or N-Acetyl Cysteine on Argemone Oil/Alkaloid-Induced Skin Tumorigenesis in Mice

Pal

Alam

Singhal

et al. 2013

Nutrition and Cancer

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“…Galjaard et al (1974) isolated under a microscope cells or clones grown on a thin plastic sheet but in most laboratories the prevalent morphological cell type (epithelioid or fibroblast-like) is established and enzyme assays are done on mixed cells or in one of the cell types, mainly fibroblast-like cells. Some enzyme activities are expressed exclusively in a particular cell type such as histidase (Barnhisel et al, 1970;Melanqon et al, 1971) and D-amino acid oxidase (Gilbert and Migeon, 1977) in epithelioid cells, and cystathionine synthetase (Uhlendorf and Mudd, 1968;Gerbie et al, 1972;Mudd and Levy, 1978) in fibroblast-like cells. Thus, large variations of enzyme activities were observed in cultured amniotic fluid cells even under carefully controlled culture conditions (Butterworth et al, 1973;Sutherland et al, 1974b;Heukels-Dully and Niermeijer, 1976).…”

Section: Introductionmentioning

confidence: 99%

Separation of amniotic fluid cell types in primary culture by percoll density gradient centrifugation

et al. 1982

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Amniotic fluid cells obtained by transabdominal amniocentesis at 15-17 weeks of gestation and cultured for 15 and 21 days were separated into three fractions by density gradient centrifugation in Percoll. Each fraction (or peak) corresponded to the following densities: peak A, 1.02-1.03 g/ml; peak B, 1.04-1.05; peak C, 1.05-1.06. Peak A was composed of both non-viable and viable cells; the latter adopted the morphology of epithelial cells in culture. Peak B contained a mixture of fibroblasts and epithelioid cells and peak C had only epithelioid cells. The variability of N-acetyl-beta-hexosaminidase and alkaline phosphatase activities was reduced in peaks B and C as compared to that of peak A and of unseparated cells suggesting that more defined and homogeneous cell types for enzymatic determinations can be obtained by centrifugation in Percoll density gradient. In prenatal diagnosis of biochemical defects, the separation of cells would permit a more precise diagnosis by eliminating enzyme variability due to the presence of different cell types or non-viable cells.

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Disorders of the Metabolism of Amino Acids and Related Compounds

Shih¹,

Mandell²

2009

Genetic Disorders and the Fetus

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Histidase function in human epidermal cells

Cited by 11 publications

References 21 publications

Protective Effect of Topical Application of α-Tocopherol and/or N-Acetyl Cysteine on Argemone Oil/Alkaloid-Induced Skin Tumorigenesis in Mice

Protective Effect of Topical Application of α-Tocopherol and/or N-Acetyl Cysteine on Argemone Oil/Alkaloid-Induced Skin Tumorigenesis in Mice

Separation of amniotic fluid cell types in primary culture by percoll density gradient centrifugation

Disorders of the Metabolism of Amino Acids and Related Compounds

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