Histidine-rich glycoprotein possesses antioxidant activity through self-oxidation and inhibition of hydroxyl radical production via chelating divalent metal ions in Fenton’s reaction

Wake, Hidenori; Takahashi, Yohei; Yoshii, Yukinori; Gao, Shangze; Mori, Shuji; Wang, Dengli; Takamatsu, Kiyoshi; Nishibori, Masahiro

doi:10.1080/10715762.2020.1825703

Cited by 14 publications

(5 citation statements)

References 45 publications

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“…In several studies, histidine, in the form of a free peptide or protein, was able to scavenge both OH • and singlet oxygen (Wade & Tucker, 1998). It was discovered that histidine‐rich glycoproteins have antioxidant potential via self‐oxidation and inhibition of OH • production via chelating metal ions in Fenton's reaction (Wake et al ., 2020). Tryptophan has been shown to be an effective antioxidant in organisms, eliminating ROS and improving resistance to free radical damage (Christen et al ., 1990).…”

Section: Resultsmentioning

confidence: 99%

Antioxidant activity and stability of endogenous peptides from farmed hybrid catfish (Clarias macrocephalus × Clarias gariepinus) muscle

Chaijan

Rodsamai

Charoenlappanit

et al. 2021

Int J of Food Sci Tech

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Summary The antioxidant activity and stability of endogenous peptides isolated from farmed hybrid catfish (Clarias macrocephalus × Clarias gariepinus) muscle (EPC) were characterised. EPC contained 69 peptides with 8–24 amino acid residues, varying hydrophobic amino acid (HBA) content and distinct sequences. Among the top five, peptides discovered in EPC were ARHSYGMLYCSCPPND, DTQAARKSDDDD, AEFPCGDRRC, AAVTEELFFAGL and LILQRRKFLRMKREKYGFIYKTHL. Histidine (16.33%) and tryptophan (15.66%) were the most common amino acids found in EPC. EPC demonstrated concentration‐dependent free radical (DPPH•/ABTS•+) and hydroxyl radical (OH•) scavenging activities, as well as metal chelating ability. The effect of pH, heating temperature and in vitro digestion on EPC's DPPH• scavenging activity was studied. Lowering the pH and increasing the heating temperature to 90 °C increased the DPPH• inhibitory activity. However, after in vitro digestion, around 44% of DPPH• inhibition was reduced. The findings confirmed that farm‐raised hybrid catfish muscle contained endogenous peptides with antioxidant properties.

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Section: Resultsmentioning

confidence: 99%

Antioxidant activity and stability of endogenous peptides from farmed hybrid catfish (Clarias macrocephalus × Clarias gariepinus) muscle

Chaijan

Rodsamai

Charoenlappanit

et al. 2021

Int J of Food Sci Tech

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“…Histidine-rich glycoprotein (HRG) was also inversely associated with the fasting C-peptide/glucose ratio and more abundant in ND individuals compared with UFMs. Notably, in relation to GPX3, HRG–GPx binding and enhancement of GPx activity, including GPX3, was recently reported [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Targeted serum proteomics of longitudinal samples from newly diagnosed youth with type 1 diabetes distinguishes markers of disease and C-peptide trajectory

et al. 2023

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Aims/hypothesis There is a growing need for markers that could help indicate the decline in beta cell function and recognise the need and efficacy of intervention in type 1 diabetes. Measurements of suitably selected serum markers could potentially provide a non-invasive and easily applicable solution to this challenge. Accordingly, we evaluated a broad panel of proteins previously associated with type 1 diabetes in serum from newly diagnosed individuals during the first year from diagnosis. To uncover associations with beta cell function, comparisons were made between these targeted proteomics measurements and changes in fasting C-peptide levels. To further distinguish proteins linked with the disease status, comparisons were made with measurements of the protein targets in age- and sex-matched autoantibody-negative unaffected family members (UFMs). Methods Selected reaction monitoring (SRM) mass spectrometry analyses of serum, targeting 85 type 1 diabetes-associated proteins, were made. Sera from individuals diagnosed under 18 years (n=86) were drawn within 6 weeks of diagnosis and at 3, 6 and 12 months afterwards (288 samples in total). The SRM data were compared with fasting C-peptide/glucose data, which was interpreted as a measure of beta cell function. The protein data were further compared with cross-sectional SRM measurements from UFMs (n=194). Results Eleven proteins had statistically significant associations with fasting C-peptide/glucose. Of these, apolipoprotein L1 and glutathione peroxidase 3 (GPX3) displayed the strongest positive and inverse associations, respectively. Changes in GPX3 levels during the first year after diagnosis indicated future fasting C-peptide/glucose levels. In addition, differences in the levels of 13 proteins were observed between the individuals with type 1 diabetes and the matched UFMs. These included GPX3, transthyretin, prothrombin, apolipoprotein C1 and members of the IGF family. Conclusions/interpretation The association of several targeted proteins with fasting C-peptide/glucose levels in the first year after diagnosis suggests their connection with the underlying changes accompanying alterations in beta cell function in type 1 diabetes. Moreover, the direction of change in GPX3 during the first year was indicative of subsequent fasting C-peptide/glucose levels, and supports further investigation of this and other serum protein measurements in future studies of beta cell function in type 1 diabetes. Graphical Abstract

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“…HRG is a glycoprotein secreted from activated platelets. It ameliorates a septic condition by suppressing excess reactive oxygen species production from neutrophils, reducing tissue damage at inflammatory sites [ 15 ]. ANXA1 inhibits neutrophil activation, migration, and infiltration, limiting inflammation in diabetic complications [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Retinal Proteome Analysis Reveals a Region-Specific Change in the Rabbit Myopia Model

Moon

Lee

et al. 2023

IJMS

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Uncovering region-specific changes in the myopic retina can provide clues to the pathogenesis of myopia progression. After imposing form deprivation myopia in the right eye of 6-week-old rabbits, we investigated the proteome profile of each retinal region (central, mid-periphery, and far-periphery retina), using accurate high-resolution mass spectrometry. Protein expression was analyzed using gene ontology and network analysis compared with that of the control, the left eyes. Among 2065 proteins detected from whole retinal samples, 249 differentially expressed proteins (DEPs) were identified: 164 DEPs in the far-periphery, 39 in the mid-periphery, and 83 in the central retina. In network analysis, the far-periphery retina showed the most significant connectivity between DEPs. The regulation of coagulation was the most significant biological process in upregulated DEPs in the far-periphery retina. Proteasome was the most significant Kyoto Encyclopedia of Genes and Genomes pathway in downregulated DEPs in the central retina. Antithrombin-III, fibrinogen gamma chain, and fibrinogen beta chain were identified as hub proteins for myopia progression, which were upregulated in the far-periphery retina. Proteomic analysis in this study suggested that oxidative stress can be the primary pathogenesis of myopia progression and that the far-periphery retina plays a role as the key responder.

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Histidine-rich glycoprotein possesses antioxidant activity through self-oxidation and inhibition of hydroxyl radical production via chelating divalent metal ions in Fenton’s reaction

Abstract: Nishibori (2020): Histidine-rich glycoprotein possesses antioxidant activity through self-oxidation and inhibition of hydroxyl radical production via chelating divalent metal ions in Fenton's reaction, Free Radical Research,

Cited by 14 publications

References 45 publications

Antioxidant activity and stability of endogenous peptides from farmed hybrid catfish (Clarias macrocephalus × Clarias gariepinus) muscle

Antioxidant activity and stability of endogenous peptides from farmed hybrid catfish (Clarias macrocephalus × Clarias gariepinus) muscle

Targeted serum proteomics of longitudinal samples from newly diagnosed youth with type 1 diabetes distinguishes markers of disease and C-peptide trajectory

Retinal Proteome Analysis Reveals a Region-Specific Change in the Rabbit Myopia Model

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