Histiocytic sarcoma with two immunohistopathologically distinct populations

Wakahashi, Kanako; Shimoyama, Manabu; Katayama, Yoshio; Minagawa, Kentaro; Yoshida, Kenji; Sasaki, Ryohei; Nakayama, Shunji; Yokozaki, Hiroshi; Yanagita, Emmy; Itoh, Tomoo; Hayashi, Yoshitake; Matsui, Toshimitsu

doi:10.1007/s12185-010-0699-1

Cited by 7 publications

(3 citation statements)

References 32 publications

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“…Necrosis was more often seen in FCR than in the BMD and may indicate a higher malignancy/faster growing tumor than in the BMD. Different morphological characteristics in different organs within one case were seen in this study and have been reported before (56). Metastatic tumor cells seem to first disseminate from the early primary lesion and then acquire additional genetic defects and often exhibit properties different from the cells of the primary lesion (57).…”

Section: Discussionsupporting

confidence: 53%

Morphological Distinction of Histiocytic Sarcoma from Other Tumor Types in Bernese Mountain Dogs and Flatcoated Retrievers

2018

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Section: Discussionsupporting

confidence: 53%

Morphological Distinction of Histiocytic Sarcoma from Other Tumor Types in Bernese Mountain Dogs and Flatcoated Retrievers

2018

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“…Although CD68 and CD163 as well as lysozyme were positive in most of the reported HS cases, a lysozyme-negative HS case was also reported. 11 That case had two populations of CD68 + CD163 -lysozyme + oval cells in cervical lymph node and CD68 + CD163 + lysozyme -spindle cells in hilar lymph node. The tumor cells in our case are quite similar to this latter cell component.…”

Section: Discussionmentioning

confidence: 88%

Histiocytic Sarcoma Originating in the Lung in a 16-Year-Old Male

Tomita

Ogura

Inomoto

et al. 2015

J Clin Exp Hematopathol

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We report a 16-year-old male with histiocytic sarcoma (HS) originating in the lung. Partial resection of the lung was performed for a 3-cm mass with a clear boundary detected in the right inferior pulmonary lobe on a health checkup. Histologically, the tumor infiltrated into the surrounding tissue, and was comprised of spindle cells, mainly, and foam cells accompanied by mild nuclear atypia. The tumor cells were immunohistochemically positive for CD68 and CD163, indicating histiocytic lineage and the MIB-1-positive rate was low. Spindle cell morphology of HS is quite rare and only 3 cases of pulmonary HS have previously been reported. 〔J Clin Exp Hematop 55(1) : 45-49, 2015〕

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“…HS is difficult to diagnose as its morphological features are highly variable (Affolter & Moore, 2002; Erich et al., 2018; Wakahashi et al., 2010). The morphologic features of HS can often mimic other tumours of mesenchymal or round cell origin such as sarcomas, plasma cell tumour, mast cell tumour, lymphoma, transmissible venereal tumour or amelanotic melanoma.…”

Section: Introductionmentioning

confidence: 99%

RNA in situ hybridisation as a molecular diagnostic technique targeting IBA‐1 and CD204 in canine histiocytic sarcoma

Engelien

Lejeune

Dark

et al. 2022

Veterinary Medicine & Sci

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Background Canine histiocytic sarcoma (HS) is an aggressive cancer with morphologically variable features; therefore, obtaining a definitive diagnosis can be challenging. Two proteins, IBA‐1, ionised calcium‐binding adapter molecule 1, and CD204, a macrophage scavenger receptor, have been shown to be specific immunohistochemical markers helpful in distinguishing HS from other tumour types with similar morphological features. Objectives This study was performed to demonstrate the use of RNA in situ hybridisation (ISH) technology allowing single‐molecule RNA visualisation in formalin‐fixed paraffin‐embedded (FFPE) tissues as a molecular tool for the diagnosis of canine HS. Methods Reverse transcription polymerase chain reaction (RT‐PCR) and western blot analysis for IBA‐1 and CD204 were performed to correlate gene expression and protein expression of these two markers in the histiocytic sarcoma DH82 cell line. RNA‐ISH for IBA‐1 and CD204 was performed on the DH82 cell line to validate the RNA‐ISH probes. RNA‐ISH and immunohistochemistry (IHC) were performed in clinical HS FFPE samples to demonstrate mRNA and protein expression of IBA‐1 and CD204. FFPE archived samples of canine round cell tumours, melanoma and anaplastic sarcoma were used as negative controls. Results RNA‐ISH and IHC showed moderate to strong expression for IBA‐1 and CD204 in the neoplastic cells in both the canine DH82 cell line and the archived canine HS samples. RNA‐ISH and IHC showed scattered positive staining in the control tumours samples, consistent with macrophagic infiltration. Conclusion RNA‐ISH for CD204 and IBA‐1 appeared to have a high specificity and sensitivity in our samples and may be an additional valuable diagnostic technique in identifying HS.

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Histiocytic sarcoma with two immunohistopathologically distinct populations

Cited by 7 publications

References 32 publications

Morphological Distinction of Histiocytic Sarcoma from Other Tumor Types in Bernese Mountain Dogs and Flatcoated Retrievers

Morphological Distinction of Histiocytic Sarcoma from Other Tumor Types in Bernese Mountain Dogs and Flatcoated Retrievers

Histiocytic Sarcoma Originating in the Lung in a 16-Year-Old Male

RNA in situ hybridisation as a molecular diagnostic technique targeting IBA‐1 and CD204 in canine histiocytic sarcoma

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