Histochemical and anisotropical aspects of the rat cementum

Vidal, Benedicto de Campos; Mello, Maria Luiza S.; Valdrighi, Luiz

doi:10.1159/000144313

Cited by 17 publications

(6 citation statements)

References 1 publication

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“…39 The presence of PG also was investigated in the predentin tissue, 40 revealing mostly the presence of CS-4 and CS-6. 41 CS also was found in rat 42 and human 43 cement. And with an immunolabeling technique, it was specifically localized on bovine cement in small pericellular spaces close to the lacunae, 44 with lumican and fibromodulin 45 also present in small amounts.…”

Section: Discussionmentioning

confidence: 73%

Dentin proteoglycans: An immunocytochemical FEISEM study

Breschi¹,

Lopes²,

Gobbi³

et al. 2002

J. Biomed. Mater. Res.

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Dentin proteoglycans are fundamental constituents of the dentin matrix and are distributed ubiquitously both in dentin and cement. They have several important functional properties; in particular, they have a fundamental role in the maintenance and the correct stabilization of collagen fibers. The use of phosphoric acid on dentin, as proposed in most common dental adhesive systems to establish a reliable bond, may affect the molecular structure of proteoglycans. The aim of this study was to evaluate, after the application of EDTA or phosphoric acid on dentin, the dentin proteoglycans with an immunocytochemical approach with high resolution SEM. For this purpose, dentin disks obtained from recently extracted human molars were etched with a 35% water solution of phosphoric acid for 15 s, 30 s, and 60 s. Control specimens were conditioned with EDTA. Specimens were immunolabeled with a monoclonal antibody antichondroitin sulfate and visualized with a goldconjugated secondary antibody. Conditioning dentin with EDTA resulted in a distinct labeling of the proteoglycans, as visualized on branching fibrillar structures in the order of 10-20 nm. The use of 35% phosphoric acid on dentin revealed a coagulation of proteoglycans after etching for 15 s while a very low labeling signal was detectable after 30 s. No labeling was obtained after etching dentin with phosphoric acid for 60 s. These results suggest that the use of 35% phosphoric acid on dentin is able to produce significant structural modifications of the dentin proteoglycans even after short application times. Additionally, when applied on the dentin surface for more than 30 s, phosphoric acid produces a dramatic decrease in proteoglycans' antigenicity, probably due to structural modifications of the three-dimensional conformation of these molecules.

show abstract

Section: Discussionmentioning

confidence: 73%

Dentin proteoglycans: An immunocytochemical FEISEM study

Breschi¹,

Lopes²,

Gobbi³

et al. 2002

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

show abstract

“…The presence of PG also was investigated in the predentin tissue,40 revealing mostly the presence of CS‐4 and CS‐6 41. CS also was found in rat42 and human43 cement. And with an immunolabeling technique, it was specifically localized on bovine cement in small pericellular spaces close to the lacunae,44 with lumican and fibromodulin45 also present in small amounts.…”

Section: Discussionmentioning

confidence: 99%

Dentin proteoglycans: An immunocytochemical FEISEM study

Breschi

Lopes

Gobbi

et al. 2002

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

Dentin proteoglycans are fundamental constituents of the dentin matrix and are distributed ubiquitously both in dentin and cement. They have several important functional properties; in particular, they have a fundamental role in the maintenance and the correct stabilization of collagen fibers. The use of phosphoric acid on dentin, as proposed in most common dental adhesive systems to establish a reliable bond, may affect the molecular structure of proteoglycans. The aim of this study was to evaluate, after the application of EDTA or phosphoric acid on dentin, the dentin proteoglycans with an immunocytochemical approach with high resolution SEM. For this purpose, dentin disks obtained from recently extracted human molars were etched with a 35% water solution of phosphoric acid for 15 s, 30 s, and 60 s. Control specimens were conditioned with EDTA. Specimens were immunolabeled with a monoclonal antibody antichondroitin sulfate and visualized with a gold-conjugated secondary antibody. Conditioning dentin with EDTA resulted in a distinct labeling of the proteoglycans, as visualized on branching fibrillar structures in the order of 10-20 nm. The use of 35% phosphoric acid on dentin revealed a coagulation of proteoglycans after etching for 15 s while a very low labeling signal was detectable after 30 s. No labeling was obtained after etching dentin with phosphoric acid for 60 s. These results suggest that the use of 35% phosphoric acid on dentin is able to produce significant structural modifications of the dentin proteoglycans even after short application times. Additionally, when applied on the dentin surface for more than 30 s, phosphoric acid produces a dramatic decrease in proteoglycans' antigenicity, probably due to structural modifications of the three-dimensional conformation of these molecules.

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“…Type III collagen, which coats type I collagen fibrils, is also present, although in considerably lower quantities. In addition to collagens, carboxylated and sulfated mucopolysaccharides (glycosaminoglycans) are present in human cementum .…”

Section: Cementum Compositionmentioning

confidence: 99%

Cementum proteins: role in cementogenesis, biomineralization, periodontium formation and regeneration

Arzate¹,

Zeichner‐David²,

Mercado³

2014

Periodontology 2000

127

140

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Destruction of the periodontium is normally associated with periodontal disease, although many other factors, such as trauma, aging, infections, orthodontic tooth movement and systemic and genetic diseases, can contribute to this process. Strategies (such as guided tissue regeneration) have been developed to guide and control regeneration using bioresorbable membranes and bone grafts. Although effective to a certain point, these strategies have the problem that they are not predictable and do not completely restore the architecture of the original periodontium. To achieve complete repair and regeneration it is necessary to recapitulate the developmental process with complete formation of cementum, bone and periodontal ligament fibers. Detailed knowledge of the biology of cementum is key for understanding how the periodontium functions, identifying pathological issues and for developing successful therapies for repair and regeneration of damaged periodontal tissue. It is the purpose of this review to focus on the role of cementum and its specific components in the formation, repair and regeneration of the periodontium. As cementum is a matrix rich in growth factors that could influence the activities of various periodontal cell types, this review will examine the characteristics of cementum, its composition and the role of cementum components, especially the cementum protein-1, during the process of cementogenesis, and their potential usefulness for regeneration of the periodontal structures in a predictable therapeutic manner.

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Histochemical and anisotropical aspects of the rat cementum

Cited by 17 publications

References 1 publication

Dentin proteoglycans: An immunocytochemical FEISEM study

Dentin proteoglycans: An immunocytochemical FEISEM study

Dentin proteoglycans: An immunocytochemical FEISEM study

Cementum proteins: role in cementogenesis, biomineralization, periodontium formation and regeneration

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