Histochemical and Chemical Studies of the Autonomic Innervation of the Ovary

Jacobowitz, David M.; Wallach, Edward E.

doi:10.1210/endo-81-5-1132

Cited by 151 publications

(77 citation statements)

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“…Similar concepts, based on results obtained from cold storage experiments on intestinal smooth muscle, have also been presented by Innes, Kosterlitz & Robinson (1957). Since adrenergic inhibitory mechanisms might occur primarily at the myenteric plexus (Jacobowitz, 1965), it might be assumed that enhancement by cold storage of the contractile response to DMPP and nicotine is due to impairment of such an inhibitory adrenergic system not only at the ultimate synaptic junction, but also at the myenteric plexus. It is possible to reject the hypothesis that the inhibitory action of cold storage is due to interference at postsynaptic effector sites, since prolonged cold storage did not modify the sensitivity of the taenia to noradrenaline, phenylephrine and isoprenaline (Shibata et al, 1970).…”

Section: Discussionsupporting

confidence: 53%

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The effect of cold storage on the adrenergic mechanisms of intestinal smooth muscle

Hattori

Kurahashi

Mori

et al. 1972

British J Pharmacology

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Summary1. In the guinea-pig taenia caecum, fluorescent adrenergic fibres terminate in both muscle layers. The density of these fibres is greater in the taenia than in the underlying circular muscle layer. The myenteric plexus and individual ganglion cells are also densely innervated by intensely fluorescent adrenergic nerve fibres. 2. After three days of cold storage, the specific fluorescence disappeared from all tissue layers of the taenia caecum and smooth muscle fibres. In contrast, cholinesterase active substances were still demonstrable in all tissue layers even after seven days of cold storage but the density of these substances was decreased. 3. Cold storage (3-7 days) decreased the tissue noradrenaline content and did not modify the cholinesterase enzyme activity (4 days). 4. In cold stored strips, the inhibitory response to nicotine, 1,1-dimethyl-4-phenylpiperazinium iodide (DMPP) or electrical transmural stimulation was abolished and enhancement of the contractile response occurred. Cold storage also inhibited the inhibitory action of tyramine. Similar results were observed after reserpine treatment. 5. In fresh taenia, the relaxation produced by nicotine, DMPP and electrical transmural stimulation was inhibited by adrenoceptor blocking agents and bretylium. In cold storage preparations, contraction produced by these stimuli was blocked by parasympathetic blocking agents and potentiated by anticholinesterase. These results indicate that the inhibitory response to these stimulants is mediated by stimulation of the adrenergic nerve system more than by non-adrenergic nerves; the excitatory effect is probably due to stimulation of cholinergic nerves. 6. These results suggest that the adrenergic mechanisms of the taenia caecum are more labile in cold storage than the cholinergic mechanisms. Thus, the inhibitory action of cold storage on the relaxation produced by nicotine, DMPP, and transmural stimulation is probably explained by selective physical degeneration of the adrenergic nerve terminal. Also, enhancement of the contractile response to these stimulants in cold stored preparations is explained by the lack of adrenergic inhibitory mechanisms.

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Section: Discussionsupporting

confidence: 53%

“…Acetylcholinesterase was stained by the thiocholine method of Jacobowitz (1965). Tissues were removed during anaesthesia and frozen with solid CO2.…”

Section: Methodsmentioning

confidence: 99%

The effect of cold storage on the adrenergic mechanisms of intestinal smooth muscle

Hattori

Kurahashi

Mori

et al. 1972

British J Pharmacology

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“…Using the histochemical method of Falek, Hillarp, Thieme & Thorpe (1962), Hamberger & Norberg (1965), Jacobowitz (1965) and Aberg & Eranko (1967) demonstrated that most of the sympathetic fibres present in the intestinal wall end in the myenteric and submucosal plexuses, where they make a dense network around non-fluorescent cholinergic cells. Only a few fluorescent fibres reach the longitudinal and circular muscle layers in which some cholinergic fibres are also present.…”

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confidence: 99%

The effect of catecholamines and sympathetic stimulation on the release of acetylcholine from the guinea‐pig colon

Beani

Bianchi

Crema

1969

British J Pharmacology

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1. In isolated guinea-pig terminal colon, the effect of sympathetic stimulation on contraction and acetylcholine release elicited by pelvic and transmural stimulation was investigated. 2. Sympathetic stimulation reduced the nerve-mediated contractile responses more than those produced by added acetylcholine. 3. Sympathetic stimulation also reduced the acetylcholine released during pelvic and transmural stimulation at low frequency. The inhibitory effect on acetylcholine released from resting colons is concealed by the simultaneous release of acetylcholine in considerable amounts from stimulated periarterial nerves which probably contain parasympathetic fibres. 4. The inhibitory effect of endogenous and exogenous catecholamines prevails when cholinergic neurones fire at low rates. It was confirmed that adrenaline is more active than noradrenaline. 5. The release of acetylcholine from unstimulated colons was for the most part maintained by nerve-conducted activity, because tetrodotoxin was able to reduce it to about one-tenth. 6. It is suggested that the sympathetic control of gastrointestinal tone and motility is exerted through two different routes: inhibition of intramural cholinergic plexuses and direct relaxation of smooth muscle cells. 7. The possible site and mechanism of action of catecholamines on intramural cholinergic structures is briefly discussed.The possible modulation of ganglionic transmission by the sympathetic system is still an open question and a matter of discussion. From careful investigations carried out on the gastrointestinal tract of rabbits and guinea-pigs, Gershon (1967) drew the conclusion that the sympathetic system exerts a direct inhibitory action on the smooth muscle without interfering with intramural ganglionic transmission of the parasympathetic outflow. Jansson & Martinson (1966) and Kewenter (1965), however, reached the opposite conclusion by examining the effect of the direct and reflex stimulation of the sympathetic nerves on the vagal control of gastric activity in the cat. Indeed, many morphological and pharmacological observations support the hypothesis that there is a sympathetic control of ganglionic transmission.

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“…Histochemical and electron microscopic studies have disclosed that muscular tissue is embedded in the mammalian ovary (Jacobowitz & Wallach, 1967;O'Shea, 1971;Okamura et al, 1972), and the contractility of that tissue has also been reported as a possible mechanism in the process of ovulation (Rocereto et al, 1969;Palti & Freund, 1972;Virutamasen et al, 1972;Gimeno et al, 1973;O'Shea & Phillips, 1974). During the course of perfusion experiments on the uterus-Fallopian tube-ovary complex of women, we found that spike potentials could be recorded from a part of the ovary.…”

mentioning

confidence: 54%

Electrical Activity Recorded From Ovarian Tissue in Perfused Human Utero-Tubo-Ovarian Unit

Tojo¹,

Fukunishi²,

Tsuchihashi³

et al. 1975

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Histochemical and Chemical Studies of the Autonomic Innervation of the Ovary

Cited by 151 publications

References 0 publications

The effect of cold storage on the adrenergic mechanisms of intestinal smooth muscle

The effect of cold storage on the adrenergic mechanisms of intestinal smooth muscle

The effect of catecholamines and sympathetic stimulation on the release of acetylcholine from the guinea‐pig colon

Electrical Activity Recorded From Ovarian Tissue in Perfused Human Utero-Tubo-Ovarian Unit

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