Histochemical and ultrastructural studies of mast cells in the intestinal mucosa and skin of the opossum Didelphis albiventris

Santos, Ana Alice D.; Machado, Conceição R. S.

doi:10.1007/bf00166104

Cited by 9 publications

(14 citation statements)

References 22 publications

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“…The ultrastructural pictures of mast cell granules revealed different electron density but no crystalline or scroll-like pattern as found in human mast cells [Drudy et al, 1991]. The same holds true for other descriptions of mast cells in marsupials as reported by Haynes [1991], Santos and Machado [1994] and Chiarini-Garcia and Pereira [1999].…”

Section: Discussionsupporting

confidence: 67%

“…Mast cells have recently been described in marsupials, especially in Didelphis, but only in lymph nodes, intestine and skin, not in the genital tract [Haynes, 1991;Santos and Machado, 1994;Chiarini-Garcia and Pereira, 1999;Chiarini-Garcia et al, 2000]. Mast cells staining metachromatically with toluidine blue were noted in the uterus of the marsupial Bettongia by Tyndale-Biscoe [1968].…”

Section: Discussionmentioning

confidence: 99%

“…While Santos and Machado [1994] described PAS-positive mast cells in the skin and intestine of Didelphis, Haynes [1991] reported Alcian blue-positive mast cells in the lymph nodes of Sminthopsis crassicaudata. In the case of M. domestica, uterine mast cells stained only with Alcian blue, while macrophages stained only PAS positive.…”

Section: Discussionmentioning

confidence: 99%

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Ultrastructural Changes in the Uterine Luminal and Glandular Epithelium during the Oestrous Cycle of the Marsupial Monodelphis domestica (Grey Short-Tailed Opossum)

Wick

Kress²

2001

Cells Tissues Organs

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Ultrastructural changes in the endometrium associated with the oestrous cycle were studied in the South American marsupial Monodelphis domestica. The most conspicuous changes include the height and the differentiation of the uterine luminal and glandular epithelium, which consists of ciliated and non-ciliated cells. The glandular epithelium attains its maximum development during oestrus, the luminal epithelium at postoestrus. A distinct increase in the number of ciliated cells can be observed during pro-oestrus, reaching a maximum number at oestrus; this is followed by a process of deciliation. The presence of solitary cilia on the apices of non-ciliated cells is very conspicuous during all oestrous stages and can best be seen on the luminal epithelium. These findings differ from the observations in eutherian mammals, where solitary cilia are only found in the immature uterus or after ovariectomy. The secretory activity of non-ciliated cells of the luminal epithelium is hardly noticeable along the apical membrane and stains only very faintly with Alcian blue. The glandular epithelium cells are filled apically with exocytotic vesicles at oestrus and early postoestrus. However, in contrast to the cervical gland cells, they hardly stain with Alcian blue, indicating that mucins of a different type must be present. Mechanisms for the remodelling of the luminal and glandular epithelium are especially conspicuous at metoestrus and early pro-oestrus and include the presence of autolysosomes, residual bodies and apoptotic bodies. In the endometrial stroma, around the uterine glands, macrophages accumulate and attain a typical oestrous stage-dependent appearance during their phagocytotic activities.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

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Ultrastructural Changes in the Uterine Luminal and Glandular Epithelium during the Oestrous Cycle of the Marsupial Monodelphis domestica (Grey Short-Tailed Opossum)

Wick

Kress²

2001

Cells Tissues Organs

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“…The ultrastructural pictures of mast cell granules showed different electron density but no crystalline or scroll-like pattern as found in human mast cells [Drudy et al, 1991]. The same holds true for the other mast cell descriptions of marsupials, as reported by Haynes [1991], Santos and Machado [1994], and Chiarini-Garcia and Pereira [1999].…”

Section: Discussionsupporting

confidence: 66%

“…Mast cells, which are regarded as immunoregulatory cells [Metcalfe et al, 1997], have recently been described in marsupials, especially in Didelphis but only for lymph nodes, intestine and skin and not for the genital tract [Haynes, 1991;Santos and Machado, 1994;Chiarini-Garcia and Pereira, 1999;Chiarini-Garcia et al, 2000]. Mast cells staining metachromatically with toluidine blue have been mentioned in the uterus of the marsupial Bettongia by Tyndale-Biscoe [1968].…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural Changes in the Cervical Epithelium during the Estrous Cycle of the Marsupial Monodelphis domestica (Grey Short-Tailed Opossum)

Wick

Kress²

2002

Cells Tissues Organs

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Ultrastructural changes in the cervical epithelium related to the estrous cycle have been studied in the South American marsupial Monodelphis domestica. The two cervices protrude with prominent papillae into the sinus vaginalis. At times of simple columnar at others of more pseudostratified character consists of two types of cells, ciliated and secretory cells. The mucosal epithelium is uniform in its entire length and shows no division into an endo- and ectocervix. The mucosa of the cervix differs from the uterine endometrium. There are no glandular structures, but the luminal epithelium shows deep invaginations underlined by dense connective tissue. The most conspicuous changes include the height and the differentiation of the cervical epithelium which attains its maximum development during estrus, where secretory cells are fully packed with large secretory granules and ciliated cells are well developed. After extruding their granules, secretory cells may transform into ciliated cells, while ciliated cells show the phenomenon of deciliation in which cilia packets are shed into the cervical lumen. This transformation process takes place mainly during post- and metestrus. The presence of solitary cilia is only noticeable during pro-estrus, being in contrast to the uterine epithelium where they appear during the whole estrous cycle.

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Development of different mast cell types in the opossum Didelphis albiventris

et al. 2003

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Previous studies have disclosed three types of mast cell in opossums: connective tissue (CTMC), mucosal (MMC), and lymphatic sinus (LSMC). In contrast to most opossum lymph nodes, the mesenteric lymph node is virtually devoid of LSMC, displaying medullary cord CTMC. The present study aimed to describe the development of these mast cell populations. Toluidine blue staining and a histochemical method for demonstrating heparin allowed the identification of immature and mature mast cells. Immature CTMC devoid of detectable heparin were rare until postnatal day 10. Mature CTMC filled with heparin-containing granules became numerous by day 30 to day 40. In the ileum, despite the presence of mature CTMC in the submucosa and mucosa (villus base), immature mast cells first appeared in the villus core by day 65 and adult features were apparent by day 100. In LSMC-containing lymph nodes, immature mast cells were found in lymphatic sinuses by day 10. Clear signs of LSMC differentiation were observed from day 20. Compared with the 10-day value, the mean diameter of cytoplasmic granules at day 40 had doubled and that at day 110 had tripled. In the mesenteric lymph nodes, immature mast cells differentiated into lymphatic sinus CTMC-like cells. After day 80, most of them were located in medullary cords. Weaning and complete maturation of mucosa preceded the differentiation of MMC. In lymph nodes, LSMC differentiation occurred in parallel with the development of the medullary region and deep cortex units.

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Histochemical and ultrastructural studies of mast cells in the intestinal mucosa and skin of the opossum Didelphis albiventris

Cited by 9 publications

References 22 publications

Ultrastructural Changes in the Uterine Luminal and Glandular Epithelium during the Oestrous Cycle of the Marsupial <i>Monodelphis domestica</i> (Grey Short-Tailed Opossum)

Ultrastructural Changes in the Uterine Luminal and Glandular Epithelium during the Oestrous Cycle of the Marsupial <i>Monodelphis domestica</i> (Grey Short-Tailed Opossum)

Ultrastructural Changes in the Cervical Epithelium during the Estrous Cycle of the Marsupial <i>Monodelphis domestica</i> (Grey Short-Tailed Opossum)

Development of different mast cell types in the opossum Didelphis albiventris

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