1983
DOI: 10.3109/10520298309066803
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Histochemical Application of Mild Alkaline Hydrolysis for Selective Elimination of O-Glycosidically Linked Glycoproteins

Abstract: A new technique to eliminate O-glycosidically linked glycoprotein (mucin-type glycoprotein) selectively has been developed. Composite paraffin sections were collodionized before and after alkaline treatment with 0.5 M NaOH in 70% ethanol; the effect of this procedure on mucosubstances was examined using the periodic acid-Schiff reaction. Exposure to alkaline hydrolysis for 72 to 144 hours at 4 C led to a complete loss of periodic acid-Schiff reactivity of epithelial mucins in rat sublingual gland, stomach and … Show more

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Cited by 48 publications
(33 citation statements)
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“…Paraffin sections were treated with 0.5 N NaOH in 70% ethanol at 4C for 7 or 14 days according to Ono et al (1983). Sections from rat testis were used as control.…”
Section: Chemical Treatmentsmentioning
confidence: 99%
See 1 more Smart Citation
“…Paraffin sections were treated with 0.5 N NaOH in 70% ethanol at 4C for 7 or 14 days according to Ono et al (1983). Sections from rat testis were used as control.…”
Section: Chemical Treatmentsmentioning
confidence: 99%
“…We have investigated both the nature of the carbohydrates and the nature of the linkage between oligosaccharide chains and protein core, to obtain information that could illustrate the possible common origin of Paneth and goblet cells. To obtain these data, lectin staining was combined at the light and electron microscopic levels with the following methods: (a) ␤ -elimination (chemical deglycosylation), which removes protein-carbohydrate linkage of the O -glycosidic type (Ono et al 1983);and (b) peptide N -glycosidase F (PNGase-F), which removes N -linked oligosaccharides (Lucoq et al 1987). …”
mentioning
confidence: 99%
“…To eliminate O-linked oligosaccharides (b-elimination reaction), the sections were incubated with 0.5 N NaOH in 70 % (v/v) ethanol at 4 ЊC, for 5 days [14]. After this treatment; staining of the incubated sections with HPA (a specific marker for O-linked oligosaccharides) was negative, whereas staining with GNA (a specific marker for N-linked oligosaccharides) was positive; and, thus, 5 days of treatment was the time required to remove O-linked oligosaccharides without modification of the N-linked oligosaccharides.…”
Section: Enzymatic and Chemical Treatmentsmentioning
confidence: 99%
“…In the first procedure, paraffin sections were treated with 0n5 N sodium hydroxide in 70 % ethanol at 4 mC for 10 d (Ono et al 1983). Acid hydrolysis was performed by immersing the sections in 0n1  hydrochloric acid for 2-3 h at 82 mC (Schauer, 1982 ;Madrid et al 1994).…”
Section: Chemical Deglycosylation Pretreatmentsmentioning
confidence: 99%