Histochemical demonstration of carbonic anhydrase activity in the uterus

Histochemical localization of carbonic anhydrase in vertebrate nervous tissue

“…Surprisingly, few studies exist on the CA distribution in the nervous system; and so far, the information is rather fragmentary and partly contradictory regarding the cellular and subcellular localization of CA. The results in the present study are in some respects at variance with a number of findings reported in earlier work [Korhonen and Korhonen, 1965;Korhonen and Hyyppa, 1967;Leder, 19661. in nervous tissue [Korhonen et al, 19641 and the present findings, most authors d o agree that the glial cell is CA-positive and the neuron CA-negative. This also is in agreement with the observation by Giacobini [1961, 19621 using a microdiver technique and the suggestions by Tschirgi [1958].…”

“…Hausler [I9581 developed an essentially new method, which seems t o be the only reliable histochemical procedure for CA demonstration in thin histological sections. In the classical work by Korhonen et al [ 19641, Hausler's method was employed for the first time in studies on the CA distribution in the nervous system; many similar studies followed [Korhonen and Korhonen, 1965;Korhonen and Hyyppa, 1967;Leder, 19661. The pC02 has a crucial influence on the excitability of the peripheral and central nervous system (review by Wyke, 1963). It was emphasized in a number of earlier publications from this laboratory that the influence of changes of pC02 on iieuronal excitability and function originates in the CA-containing glial cells [Svaetichin et al, 1961[Svaetichin et al, , 1963[Svaetichin et al, , 1965Negishi and Svaetichin, 19661. In experiments with frog nerve fiber, spinal ganglion cell, the retina [Negishi and Svaetichin, 19661, and cat cerebral cortex [Negishi et al, 19631, it was demonstrated that in Diamox-treated animals, the pCOz changes were without effect on the nervous function.…”

Histochemical Localization of Carbonic Anhydrase in the Female Genitalia of Pigs during the Oestrous Cycle

“…Carbonic anhydrase activity in the genitalia of several species has been found to increase during the luteal phase of the oestrous cycle [15,27], and even to disappear dur ing pro-oestrus [28], The histological patterns or the intensity of carbonic anhydrase activity in all areas of the female porcine genitalia as examined in the present study did not show variations with the phases of the oestrous cycle, although the method employed does not allow accurate quantitation.…”