Histochemical demonstration of terminal deoxynucleotidyl transferase in leukemia

Hecht, T.; Forman, Stephen J.; Winkler, Ursula S.; Santos, Scarlath Ohana Penna dos; Winkler, K; Carlson, Fumiko; Maslow, William C.; Borer, William Z.; Blume, Karl G.

doi:10.1182/blood.v58.4.856.856

Cited by 29 publications

(2 citation statements)

References 9 publications

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“…A previous study [26] on a small number of patients indicated concordance between the biochemical. indirect immunofluorescent, and immunoperoxidase methods for TdT using a different commercial primary antibody.…”

Section: Discussionsupporting

confidence: 63%

Terminal deoxynucleotidyl transferase activity in acute leukemia: A study of 100 cases comparing an immunoperoxidase (PAP) vs immunofluorescent method

et al. 1986

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A comparison between the immunofluorescent (IF) method for terminal deoxynucleotidyl transferase (TdT) activity and the immunoperoxidase (IP) method by peroxidase-anti-peroxidase (PAP) technique was done for 100 cases of acute leukemia. For the acute lymphoblastic leukemias (ALL) there was agreement in 93% of the cases. However, the IP method detected 51/55 (93%) TdT+ cases versus 47/55 (85%) by the IF method. For the acute nonlymphocytic leukemias (ANLL), there was an agreement in 89% of the cases. The IP method detected 8/36 (22%) TdT-positive cases while IF detected 4/36 (11%) positive cases. If a figure of 10% TdT+ cells is considered significant in the marrow of the ANLLs, then the IP method would detect eight additional cases for a total of 16/36 (44%) TdT+ cases. This latter figure questions the ability of the IP TdT assay as a single test adequately to determine the lineage of a cell line. It may be rather that TdT is a marker that is expressed in a stem cell.

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Section: Discussionsupporting

confidence: 63%

Terminal deoxynucleotidyl transferase activity in acute leukemia: A study of 100 cases comparing an immunoperoxidase (PAP) vs immunofluorescent method

et al. 1986

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“…The methods of cytochemical staining with peroxidase, a-naphthyl butyrate esterase, chloroacetate esterase, acid phosphatase, and toluidine blue were those previously reported [8, [19][20][21]. Immunoperoxidase staining for TdT was according to the method of Hecht et al [22]. Conventional immunologic techniques for surface immunoglobulin and E-rosette were performed as described elsewhere [ 13,231.…”

Section: Materials and Methods General Methodsmentioning

confidence: 99%

Acute lymphocytic leukemia: Correlation of clinical features with immunocytochemical classification

Twu¹,

Li²,

Smithson³

et al. 1987

American J Hematol

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Many immunologic studies of acute lymphocytic leukemia (ALL) during the past decade have demonstrated the close correlation of immunologic phenotypes of ALL subclasses with the clinical presenting features and prognosis. However, the clinical application of conventional immunologic techniques had been very limited because of the requirement of a fresh sample to prepare the mononuclear cell suspensions for study. We studied 81 cases of ALL using immunoperoxidase stain for nuclear terminal deoxynucleotidyl transferase (TdT) and immunoalkaline phosphatase stain for surface markers (using monoclonal antibody J5 for common ALL antigen [CALLA], Leu-1 for pan-T antigen, and B1 for pan-B antigen) on air-dried smears. The cases were classified as common ALL (TdT+, CALLA+, pan-T-, and pan-B-) (41 cases), null-ALL (TdT+, CALLA-, pan-T-, and pan-B-) (19 cases), T-ALL (TdT+, CALLA-, pan-T+, and pan-B-) (nine cases), B-ALL (TdT-, CALLA-, pan-T-, and pan-B+) (six cases), pre-B-ALL (TdT+/-, CALLA+, pan-T-, and pan-B+) (four cases), or pre-T-ALL (TdT+, CALLA+, pan-T+, and pan-B-) (two cases). This subtyping of ALL correlated well with known clinical presenting features, prognosis, chromosome analysis in 35 cases with an abnormal clone, and conventional immunologic typing in 38 cases. The data suggest that these simple and practical immunocytochemical stains can be used for immunologic subclassification of ALL.

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Megakaryoblastic transformation of chronic granulocytic leukemia

Travis

Banks

et al. 1987

Cancer

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Three cases of megakaryoblastic transformation of chronic granulocytic leukemia (CGL) are reported. In each case, the leukemic transformation had morphologic features suggesting megakaryocytic differentiation. This was confirmed by positive immunostaining with a monoclonal antibody (HP1-1D) specific for platelet and megakaryocyte glycoprotein IIb/IIIa antigen, which was expressed by the majority of the leukemic blasts in all three cases. Cases with evidence of multilineage differentiation of the leukemic transformation were excluded. A striking feature in two patients was the manifestation of lytic bone lesions and soft tissue masses at presentation. A biopsy of a lytic bone lesion and soft tissue mass in one patient revealed a megakaryoblastic leukemic infiltrate, which by immunocytochemical staining was positive for the megakaryocytic markers, glycoprotein IIb/IIIa antigen, and Factor VIII (von Willebrand factor) antigen. In contrast to granulocytic sarcomas, the megakaryoblastic sarcoma did not stain cytochemically for chloroacetate esterase. The mean survival after acute transformation was 5.3 months. The three cases of megakaryoblastic transformation represented a significant proportion of all CGL blastic transformation cases (ten cases) evaluated by bone marrow examination in our institution during a 13-month period. Megakaryoblastic transformation of CGL may occur more frequently than has been appreciated, and can present as lytic bone lesions or as soft tissue megakaryoblastic sarcomas.

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Histochemical demonstration of terminal deoxynucleotidyl transferase in leukemia

Cited by 29 publications

References 9 publications

Terminal deoxynucleotidyl transferase activity in acute leukemia: A study of 100 cases comparing an immunoperoxidase (PAP) vs immunofluorescent method

Terminal deoxynucleotidyl transferase activity in acute leukemia: A study of 100 cases comparing an immunoperoxidase (PAP) vs immunofluorescent method

Acute lymphocytic leukemia: Correlation of clinical features with immunocytochemical classification

Megakaryoblastic transformation of chronic granulocytic leukemia

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