Histochemical distribution of cholinesterases in the testis, epididymis and vas deferens of the rat

Risley, Paul L.; Skrepetos, Chris N.

doi:10.1002/ar.1091480213

Cited by 60 publications

(24 citation statements)

References 29 publications

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“…[38] as well as [39] have considered it a modification of a single cell type at different phases of its metabolic cycle. [40][41][42][43][44] believed that, these cells are holocrine secretory cells as they lack stereocilia and have large clear vacuoles with finely granulated material. However [45,46] suggested that, they are dying principal cells, a suggestion which seem to be the most plausible for the camel epididymis.…”

Section: Discussionmentioning

confidence: 99%

Morphological Studies on the Epididymal Duct of the One-Humped Camel (Camelus Dromedaries)

Zayed¹,

Aly²,

Ibrahim³

et al. 2012

OJVM

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The present work was carried out on 20 epididymes of sexually mature one humped camel to elucidate the gross anatomical, scanning electron microscopical and light microscopical features of the epididymal duct. Anatomically, the epididymal duct of camel consists of three parts i.e. head, body and tail. Histomorphologically, the epididymal duct is subdivided into initial, middle and terminal segments, of which the middle segment is further subdivided into proximal, intermediate and distal parts. The epithelium lining the epididymal duct is ciliated pseudostratified columnar comprising mainly five cell types, namely principal, basal, apical, dark and halo cells. It displays numerous intraepithelial glands in the middle segment. In general, there is a gradual increase in the total diameter and decrease in the epithelial height of the epididymal duct from the initial to the terminal segments; that may, mechanically, facilities passage of the sperms toward the terminal segment. The lamina propria of the epididymal duct contains a layer of interlacing elastic fibers which may allow adequate elasticity of the duct that could help in its expansion. Moreover it is surrounded by numerous layers of circularly and obliquely arranged smooth muscle fibers which always increase in thickness toward the terminal segment.

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Section: Discussionmentioning

confidence: 99%

Morphological Studies on the Epididymal Duct of the One-Humped Camel (Camelus Dromedaries)

Zayed¹,

Aly²,

Ibrahim³

et al. 2012

OJVM

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“…In their histochemical studies of the epididymis, Baumgarten et al (1968) and Sjostrand (1965) found an abundance of CA fluorescent fibers. Risley and Skrepetos (1964), studying rat epididymis, found AChE positive fibers in the body and tail of the epididymis, but not in the head. Sjostrand, Baumgarten and Holstein, and others have concluded that CA fluorescent fibers have an important role in spermatozoa transport in the vas deferens during emission.…”

Section: Discussionmentioning

confidence: 99%

A Histochemical Investigation of the Distribution of Adrenergic and Cholinergic Nerves in the Human Male Genital Organs

Shirai

Sasaki

Rikimaru³

1973

Tohoku J. Exp. Med.

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-A histochemical studies clarified the peripheral distribution of adrenergic and cholinergic nerves in the human male genital organs. In the human testis, there was a minimal distribu tion of catecholamine fluorescent fibers and acetylcholinesterase positive fibers around the blood vessels and in the interstitium. In the epididymis and the vas deferens, both nerve fibers were abundant in the muscle layer and interstitium. However, no difference in the distribution of these nerve fibers was noted according to the anatomical site of these organs. In the prostate, acetylcholinesterase positive fibers were abundant; but catecholamine fluorescent fibers were relatively few, and found in the interstitium and the muscle layer. These two types of nerve fibers were not found in the epithelial cell layer of the organs. catecho lamine; acetylcholinesterase; testis; epididymis; vas deferens; prostate Little is known about the innervation of the human male gential organs, especially of the testis, epididymis, vas deferens, prostate and penis, though the functions of these organs, i.e., spermatogenic function, ejaculation and erection are well known to be controlled by the nerves. There have been few histochemical studies on the distribution and course of the peripheral nerve supply to these organs.The silver staining method, which was used in the past for studies of the nervous system, is inadequate for accurate classification of the sympathetic and parasym pathetic peripheral nerve distribution in the genital organs.Recently, however, it has become possible to use catecholamine (CA) fluores cence as a histochemical index in order to demonstrate the distribution of adrenergic nerves. The distribution of cholinergic nerves has been demonstrated, using acetylcholinesterase (ACNE) as an index.The authors have already shown histochemically the localization of adrener gic and cholinergic nerves in the human penis . In the present study, the authors have demonstrated histochemically the localization of adrener-

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“…The latter is believed to be active in holocrine secretion in the rat [Martan and Risley, 1963a, b;Risley and Skrepetos, 1964], mouse [Martan and Allen, 1964] and man [Martan, Risley and Hruban, 1964]. The criteria used to identify holocrine cells in electron micrographs are generally a lack of stereocilia, the presence of large clear vacuoles, filled with a finely granular material in the apical cytoplasm, and an overall greater density of the cytoplasm than that of the principal cells.…”

Section: Non-ciliated Type II Cellsmentioning

confidence: 99%

THE FINE STRUCTURE OF THE EPITHELIAL LINING OF THE EXCURRENT DUCT SYSTEM OF THE TESTIS OF THE DOMESTIC FOWL (GALLUS DOMESTICUS)

Tingari

1972

Exp Physiol

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Ultrastructurally, at least five different cell types were identified in the genital duct system of the cock, namely (1) low cuboidal cells lining the rete testis, (2) and (3) nonciliated type I and ciliated cells, lining the ductuli efferentes and narrow connecting ductules, (4) non-ciliated type II cells, lining the wide connecting ductules, ductus epididymidis and ductus deferens, and (5) basal cells found mainly with type II cells.The low cuboidal cells have an imbricated arrangement which, in a tissue section, influences the interpretation of their shape as well as their luminal and basal connections; squamous, cuboidal, and other shapes were observed. They possess a large Golgi complex, many small mitochondria, a few profiles of rough endoplasmic recticulum and electron dense cytoplasmic bodies. The fine structure indicates an active secretory function; the secretion is probably of a protein-rich type. The cells appear to be capable of taking up spermatozoa from the lumen. There is a rich distribution of nerves in the subepithelial tissue. This could indicate a contractile function of the rete cells; its significance has been discussed.The non-ciliated type I cells are characterized by the presence of electron-dense wormlike structures which are connected to the surface or scattered in the apical cytoplasm. Their luminal border has many microvilli, some of which form stereocilia. There is an abundance of mitochondria and smooth endoplasmic reticulum; these are evidences of metabolic and secretory activities respectively, but the nature of the secretion is unknown. Lysosomal bodies of many types are scattered throughout the cytoplasm of the non-ciliated type I cell. The worm-like bodies may be concerned with the uptake from the lumen.The main ultrastructural features of the non-ciliated type II cells are similar to those of protein secreting cells like the exocrine pancreatic acini and salivary glands. There is an abundance of distended rough and transitional endoplasmic reticulum, a well developed Golgi complex, smooth vesicles and electron-dense secretory granules in various stages of formation. Mature granules are discharged by the fusion of their membranes with the cell membrane of the secretory surface. There is an intimate association between the membranes limiting the endoplasmic reticulum and those of the mitochondria. Dense vacuolated bodies are present in the cytoplasm which are probably lysosomes. The cytoplasmic fibrillar content of these cells and the rich innervation in the subepithelial tissue suggest a contractile function.The ciliated cells possess a small Golgi complex and many mitochondria, slightly smaller than those of the type I cells and concentrated mainly in the supranuclear region. In view of the presence of motile cilia, and probably the fibrous cytoplasmic bundles, the ciliated cells may function in moving spermatozoa along the excurrent ducts. The cells are non-secretory but could play a role in the resorption of fluids secreted by the seminiferous tubules in an analogous way to...

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Histochemical distribution of cholinesterases in the testis, epididymis and vas deferens of the rat

Cited by 60 publications

References 29 publications

Morphological Studies on the Epididymal Duct of the One-Humped Camel (Camelus Dromedaries)

Morphological Studies on the Epididymal Duct of the One-Humped Camel (Camelus Dromedaries)

A Histochemical Investigation of the Distribution of Adrenergic and Cholinergic Nerves in the Human Male Genital Organs

THE FINE STRUCTURE OF THE EPITHELIAL LINING OF THE EXCURRENT DUCT SYSTEM OF THE TESTIS OF THE DOMESTIC FOWL (GALLUS DOMESTICUS)

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