Histochemical localization of glycolytic enzymes, alcohol and secondary-alcohol dehydrogenases in the testes of buffaloes, goats and rams

Bilaspuri, G. S.; Guraya, Sardul S.

doi:10.1017/s0021859600037813

Cited by 2 publications

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“…This classifica-increases the precision and objectivity. Moreover, tion system usually consists of eight stages; the reliability of this criterion is indispensable in however, in order to increase the specificity of this investigating the behaviour of various spermato-G. S. BlLASPTJRI AND S. S. GlTRAYA genie cells as well as the effects of various chemicals on spermatogenesis (Swierstra, Whitefield & Foote, 1964;Steinberger, 1971;Clermont, 19721. Some aspects of tostes of goats have been investigated in our laboratory (Bilaspuri & Guraya, 1983, 1984, but, in spite of the economic importance of this species as a farm animal, so far no work has been done on its SEC and spermatogenesis. In addition, among mammals the efficiency of spermatogenesis has been worked out only in bulls (Berndtson & Desjardins, 1974) and buffaloes (Bilaspuri & Guraya, 1980).…”

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confidence: 99%

The seminiferous epithelial cycle and spermatogenesis in goats (Capra hircus)

Bilaspuri

Guraya

1984

J. Agric. Sci.

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The development of the acrosomic system and the spermatid nucleus were used to define 14 stages of the seminiferous epithelial cycle in goats; these stages provided a basis for the examination of the behaviour of different spermatogenic cells which gave an idea of the efficiency of spermatogenesis. Eighteen steps of acrosome development (spermiogenesis) were observed in testicular material stained with periodic acid-Schiff. The first 14 steps were used to classify SEC into 14 (I-XIV) stages which in turn were employed to study the pattern of differentiation of spermatogenic cells by counting them in each stage of the cycle. Three generations of type A (A^ A 2 , A 3 ), one generation of type intermediate (In) and two generations of type B (Bj, B 2 ) spermatogonia could be distinguished. A 1 spermatogonia divided primarily in stages IX-X to produce A 2 spermatogonia which in turn divided in stages XII-XIII to produce A 3 spermatogonia and A, spermatogonia. A 3 spermatogonia divided in stage XIV to produce In spermatogonia whereas A x spermatogonia did not divide till the next cycle but underwent 26-3 % degeneration. In spermatogonia divided to form Bi spermatogonia in stages III-V which further divided to produce B 2 spermatogonia in stage VI. Types A 3 , In and B x spermatogonia showed 15-0, 25-0 and 25-8% degeneration respectively. B 2 spermatogonia divided in stages VII-VIII to produce double the number of primary spermatocytes which persisted without any degeneration till stage XIII of the following cycle and divided at the beginning of stage XIV to form double the number of secondary spermatocytes. These cells divided at the end of stage XIV to form less than double the number of young round spermatids, showing 10-4 % degeneration. It is concluded that the development of the acrosomic system as well as the spermatid nucleus could be conveniently used to study the behaviour of spermatogenic cells and that the process of spermatogenesis was less efficient than thought previously.

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Section: Introductionmentioning

confidence: 99%

The seminiferous epithelial cycle and spermatogenesis in goats (Capra hircus)

Bilaspuri

Guraya

1984

J. Agric. Sci.

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Histochemical localization of enzymes of various metabolic pathways in the testes of buffaloes, goats and rams

Bilaspuri

Guraya

1984

J. Agric. Sci.

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Isocitrate dehydrogenase (ICDH), succinate dehydrogenase (SDH), malate dehydrogenase (MDH), glutamate dehydrogenase (GDH), /Miydroxybutyrate dehydrogenase (/?-0H-BDH) and glucose-6-phosphate dehydrogenase (G-6-PDH) were histochemically located in the testes of buffaloes, goats and rams. The enzyme activities varied with the enzyme, species and cell type. The activities in the seminiferous tubules were correlated with the stages of seminiferous epithelial cycle (SEC). During this cycle, the activities in the Sertoli cells, spermatogonia and spermatocytes remained unaltered in contrast to those in the spermatids. The activities of SDH, ICDH and MDH were relatively greater in buffalo, while goat and ram resembled each other quite closely. ICDH and MDH preferred NADP to NAD. In the three species, the activities of ICDH, SDH and MDH generally followed an increasing order. G-6-PDH was greater in the interstitial tissue of buffalo than in goat and ram; the maximum activity of this enzyme in each species was found in the spermatogonia. In comparison with G-6-PDH, GDH was less evident in the interstitial tissue of buffalo and goat; Sertoli cells and spermatogonia also showed relatively less MDH activity whereas the other germ cells may have relatively less, similar or more, GDH activity depending on the species. /?-OH-BDH activity was similar in the interstitial tissue of the three species, but in the seminiferous tubule, the activity was less in goat. But for GDH and /?-OH-BDH which could show different results, the activities of other enzymes generally decreased from spermatogonia through spermatocytes to spermatids but increased during spermiogenesis. In spermatozoa, the enzymes were observed only in the mid-piece. The possible physiological significance of the results is discussed in relation to different metabolic pathways.

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Histochemical localization of glycolytic enzymes, alcohol and secondary-alcohol dehydrogenases in the testes of buffaloes, goats and rams

Cited by 2 publications

References 13 publications

The seminiferous epithelial cycle and spermatogenesis in goats (Capra hircus)

The seminiferous epithelial cycle and spermatogenesis in goats (Capra hircus)

Histochemical localization of enzymes of various metabolic pathways in the testes of buffaloes, goats and rams

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