Histochemical model studies of enzyme activity after thermal damage

Abstract

The reliability of histochemical determinations of the enzyme activity after thermal damage has been studied with the aid of two model systems. Polyacrylamide films and erythrocyte ghosts containing either beta-glucuronidase or alkaline phosphatase, were submitted to heating and the activities retained were assessed both biochemically and histochemically. For the enzymes studied, the results show that tissue alterations induced by heat can influence histochemical reaction procedures, and that with these model systems, factors which are important for the histochemical quantitation of enzyme activities in thermally damaged tissues can be evaluated quantitatively. Potentialities of these model systems in the study of evaluating thermal damage through histochemical enzyme activity determinations, are discussed.

Χαρακτηριστικά Των Δηκτικών Τραυματικών Κακώσεων Και Προσδιορισμός Της Ηλικίας Των Τραυματικών Κακώσεων Στα Ζώα

“…Bleeding, cahexia, cold environment and cranial fractures do not delay the inflammatory reactions (Benz 1994, Raekallio 1984, Burg 1972. Some other investigators studied burns using enzymatic histochemistry (Prosperi et al 1982, Cejkova et al 1989, Stal et al 1995.…”

“…• Χρησιµοποιούνται µε µικρές τροποποιήσεις, για τον προσδιορισµό της ηλικίας εγκαυµάτων, καθώς και τραυµατικών κακώσεων οστών, χόνδρων και µυών. (Miyaki K.,et al, 1994, Prosperi E., et al 1982, Perper A.J., 1980, Sugimura K.,et al 1980 Παρά τα όσα παραπάνω έχουν αναφερθεί, για την εξαγωγή σωστού συµπεράσµατος τόσο για την ηλικία όσον και για την προ-ή µεταθανάτια δηµιουργία ενός τραύµατος, πρέπει απαραίτητα να ληφθούν υπόψη και τα ευρήµατα της νεκροψίας -νεκροτοµής που αναφέρονται στο ίδιο επιστηµονικό πεδίο.…”