Histochemical studies of noradrenergic innervation of the liver in untreated and daunomycin-pretreated guinea-pigs

Mazzanti, L; Tacca, M. Del; Breschi, Maria Cristina

doi:10.1007/bf00511206

Cited by 17 publications

(6 citation statements)

References 5 publications

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“…B y contrast. several other groups ha\'e described intraacinar nerves using similar histochemical techniques in the livers of' guinea pigs, cats, rabbits, cows, pigs, primates and humans (18)(19)(20)(21)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32). Metz animal species, ranging from a negligible amount i n the rat to the presence of a rich supply in the guinea pig.…”

Section: Resultsmentioning

confidence: 99%

Localization of adrenergic and neuropeptide tyrosine-containing nerves in the mammalian liver

et al. 1989

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The distribution of adrenergic nerves in guinea pig and rat liver was studied by the immunolocalization of fibers containing tyrosine hydroxylase and dopamine beta-hydroxylase, enzymes involved in the synthesis of catecholamines. In both species, adrenergic fibers were identified within portal tracts, often in close proximity to hepatic artery branches. In guinea pig liver, but not rat liver, abundant intraacinar fibers were identified; fibers were also seen within the walls of terminal hepatic vein radicles and larger hepatic veins. The presence of peptidergic nerves containing the regulatory peptide neuropeptide tyrosine and the C-flanking peptide CPON was investigated by indirect immunofluorescence. The distribution of these nerves was similar to that of tyrosine hydroxylase- and dopamine beta-hydroxylase-positive nerves and showed the same species difference. The effector sympathetic nature of tyrosine hydroxylase- and neuropeptide tyrosine-positive fibers in rat liver was confirmed by chemical denervation studies using 6-hydroxydopamine.

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Section: Resultsmentioning

confidence: 99%

Localization of adrenergic and neuropeptide tyrosine-containing nerves in the mammalian liver

et al. 1989

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“…The aminergic innervation of the normal liver has been examined in depth in various mammalian species (Mazzanti et al, 1977;Fuller et al, 1981;Moghimzadeh et al, 1982) including man (Moghimzadeh et al, 1982;Kyosola et al, 1985). In the present study we only investigated the distribution of TH-IR nerves.…”

Section: Discussionmentioning

confidence: 96%

The absence of autonomic perivascular nerves in human colorectal liver metastases

Ashraf

Crowe²,

Loizidou³

et al. 1996

Br J Cancer

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Summary The peptidergic/aminergic innervation of normal liver and tumour blood vessels was investigated in order to determine vascular control with a view to improving the efficacy of hepatic arterial cytotoxic infusion in the treatment of colorectal liver metastases. Selected areas of liver metastases and macroscopically normal liver from resection specimens (n= 13) were studied using light microscope immunohistochemistry for the presence of protein gene product 9.5 (PGP), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), calcitonin gene-related peptide (CGRP), substance P (SP) and tyrosine hydroxylase (TH). The ultrastructure of blood vessels supplying liver metastases and their perivascular innervation were also examined by transmission electron microscopy. In the normal liver, perivascular immunoreactive nerve fibres containing PGP, NPY and TH were observed around the interlobular blood vessels and along the sinusoids and the central vein of the hepatic lobule. The greatest density of immunoreactive nerve fibres was seen for PGP, followed (in decreasing order) by NPY and TH. VIP, SP and CGRP immunoreactivity was observed only in nerve bundles associated with the large interlobular blood vessels. In contrast, no perivascular immunoreactive nerves were observed in colorectal liver metastases. Electron microscopy confirmed the absence of perivascular nerves in liver metastases. In addition, it showed that the walls of these blood vessels were composed of a layer of endothelial cells surrounded by an incomplete or, very rarely in the periphery of the tumour, a complete, layer of synthetic phenotype of smooth muscle-like cells. These results imply that the blood vessels supplying liver metastases are bereft of normal neuronal regulation; whether there is a role for endothelial cell control of blood flow in these vessels is not yet known.

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“…Most aminergic nerves course adjacent to the vasculature of the portal tract with more fibers being associated with the hepatic arteries than the portal veins or bile ducts (Figs. 1 and 2) (Yamada, 1965; Ungvary and Donath, 1969; Anufriev et al, 1973; Forssmann and Ito, 1975, 1977; Skaaring and Bierring, 1976; Mazzanti et al, 1977; Nobin et al, 1977; Uno, 1977; Reilly et al, 1978; Burt et al, 1989; Oda et al, 1990; Feher et al, 1991; Goehler and Sternini, 1991; Lin et al, 1995). In general, central and sublobular hepatic veins lack innervation but aminergic nerves have been demonstrated in the walls of the larger hepatic veins (Mikhail and Saleh, 1961; Ungvary and Donath, 1969; Nobin et al, 1977; Reilly et al, 1978; Metz and Forssmann, 1980b; Fuller et al, 1981; Burt et al, 1989).…”

Section: Aminergic Innervationmentioning

confidence: 99%

“…The intralobular distribution of aminergic nerves is subject to considerable species variation (Yamada, 1965; Ungvary and Donath, 1969; Anufriev et al, 1973; Forssmann and Ito, 1975, 1977; Skaaring and Bierring, 1976; Mazzanti et al, 1977; Nobin et al, 1977, 1978; Uno, 1977; Reilly et al, 1978; Metz and Forssmann, 1980b; Moghimzadeh et al, 1982; Ohata, 1984; Ueno et al, 1988; Burt et al, 1989; Bioulac‐Sage et al, 1990; Feher et al, 1991; Lin et al, 1995). In most mammals, including humans, these intralobular nerves course from the portal space through the space of Disse (Fig.…”

Section: Aminergic Innervationmentioning

confidence: 99%

Anatomy of efferent hepatic nerves

McCuskey

2004

Anat. Rec.

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The role of neural elements in regulating blood flow through the hepatic sinusoids, solute exchange, and parenchymal function is incompletely understood. This is due in part to limited investigation in only a few species whose hepatic innervation may differ significantly from humans. For example, most experimental studies have used rats and mice having livers with little or no intralobular innervation. In contrast, most other mammals, including humans, have aminergic and peptidergic nerves extending from perivascular plexus in the portal space into the lobule, where they course in Disse's space in close relationship to stellate cells (fat storing cells of Ito) and hepatic parenchymal cells. While these fibers extend throughout the lobule, they predominate in the periportal region. Cholinergic innervation, however, appears to be restricted to structures in the portal space and immediately adjacent hepatic parenchymal cells. Neuropeptides have been colocalized with neurotransmitters in both adrenergic and cholinergic nerves. Neuropeptide Y (NPY) has been colocalized in aminergic nerves supplying all segments of the hepatic-portal venous and the hepatic arterial and biliary systems. Nerve fibers immunoreactive for substance P and somatostatin follow a similar distribution. Intralobular distribution of all of these nerve fibers is species-dependent and similar to that reported for aminergic fibers. Vasoactive intestinal peptide and calcitonin gene-related peptide (CGRP) are reported to coexist in cholinergic and sensory afferent nerves innervating portal veins and hepatic arteries and their branches, but not the other vascular segments or the bile ducts. Nitrergic nerves immunoreactive for neuronal nitric oxide (nNOS) are located in the portal tract where nNOS colocalizes with both NPY-and CGRP-containing fibers. In summary, the liver is innervated by aminergic, cholinergic, peptidergic, and nitrergic nerves. While innervation of structures in the portal tract is relatively similar between species, the extent and distribution of intralobular innervation are highly variable as well as species-dependent and may be inversely related to the density of gap junctions between contiguous hepatic parenchymal cells. 2004 Wiley-Liss, Inc.

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Histochemical studies of noradrenergic innervation of the liver in untreated and daunomycin-pretreated guinea-pigs

Cited by 17 publications

References 5 publications

Localization of adrenergic and neuropeptide tyrosine-containing nerves in the mammalian liver

Localization of adrenergic and neuropeptide tyrosine-containing nerves in the mammalian liver

The absence of autonomic perivascular nerves in human colorectal liver metastases

Anatomy of efferent hepatic nerves

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