Histochemical studies of rat ovarian follicular cells in vitro

Fischer, Theodore V.; Kahn, Raymond H.

doi:10.1007/bf02615976

Cited by 35 publications

(5 citation statements)

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“…To ascertain the effect of oocyte presence and of LH addition on the progesterone secretion rate by the cultures, two-way analysis of variance (Sokal & Rohlf, 1969) (Nicosia & Mikhail, 1975). The present results (Tsafriri & Channing, 1975a, b (Channing, 1969), monkey (Channing, 1970a), porcine (Channing, 1970b), rabbit (Nicosia, 1972) and rat (Fischer & Kahn, 1972) follicles must therefore be explained by a mechanism other than removal of the egg from the granulosa cells. Ledwitz-Rigby et ai (1977) have observed that addition of follicular fluid obtained from small-and medium-sized follicles can in¬ hibit spontaneous luteinization of porcine granulosa cells.…”

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confidence: 55%

Lack of an inhibitory influence of oocytes upon luteinization of porcine granulosa cells in culture

Channing¹,

Tsafriri²

1977

Reproduction

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Nalbandov (1972) suggested that the ovum produces a "luteostatic" substance which prevents luteinization of granulosa cells. This suggestion was derived from three lines of observation: (1) surgical ovectomy in vivo induced luteinization and increased the output of progesterone into the ovarian venous blood of rabbits (El-Fouly, Cook, Nekola & Nalbandov, 1970) and pigs (Nalbandov, 1972); (2) rat granulosa cell monolayers cultured with only a few oocytes luteinized, whereas those cultured in the presence of many ova retained their granulosa cell morphology (Nekola & Nalbandov, 1971); and (3) oocytic degeneration on the 3rd day of culture of rat follicles coincided with morphological luteinization of the mural granulosa (Stoklosowa & Nalbandov, 1972).To test this hypothesis porcine granulosa cells were cultured with and without the addition of cumulus-enclosed porcine oocytes. Granulosa cells and oocytes were collected from medium and large follicles (3-12 mm) of pig ovaries removed at a local slaughterhouse. Within 20-30 min after death the ovaries were excised and placed in 0-9% (w/v) NaCl containing 100 i.u. penicillin/ml, and 100 pg streptomycin/ml and kept at room temperature until culture 3-5 h later. Details of granulosa cell and oocyte collection have been described elsewhere (Channing & Ledwitz-Rigby, 1975 ; Tsafriri & Channing, 1975a, b (Channing, 1974). The serum contained 66 pg total oestrogens/ml as measured by radio¬ immunoassay (Channing & Coudert, 1976). The progesterone content of the serum was 3-8-4-1 ng/ ml, using a modification (Channing & Coudert, 1976) of the radioimmunoassay developed by Thorneycroft & Stone (1970). Morphological luteinization and the number of cells in the cultures were assessed as described elsewhere (Channing, 1970a, b). To some of the cultures cumulusenclosed oocytes (50/culture) and/or ovine LH (NIH-LH-S19 ; 5 pg/ml) were added. Each treatment combination was tested in eight replicate cultures. At 12-h intervals the medium was changed and progesterone was determined by radioimmunoassay using an antiserum against 1 la-hydroxyprogesterone-BSA conjugate.The progesterone secretion rate by granulosa cell cultures with and without oocytes and LH during the 12 h intervals is presented in (Nicosia & Mikhail, 1975

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confidence: 55%

Lack of an inhibitory influence of oocytes upon luteinization of porcine granulosa cells in culture

Channing¹,

Tsafriri²

1977

Reproduction

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“…There has also been a general increase in use of cell and organ cultures for studying gonadal function (Channing and Steinberger, 1970;Fainstat, 1973). Granulosa cells obtained from preovulatory follicles of the mare (Channing, 1966(Channing, , 1969bChanning and Grieves, 1969), human (Channing, 1969c), monkey (Channing, 1970b), pig (Schomberg, 1969;Channing, 1970c), and rat (Fischer and Kahn, 1972) spontaneously luteinize morphologically and secrete elevated amounts of progestin in culture, thus justifying their use as a "test-tube" corpus luteum. Granulosa cells obtained from preovulatory monkey follicles also luteinize, as seen at the fine structural level .…”

Section: Discussionmentioning

confidence: 99%

Physiology and Genetics of Reproduction

1974

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“…Culture media were frozen and stored for steroid analysis. Every second day 1 monolayer was stained by May-Griinwald-Giemsa for cytological observation and another monolayer was submitted to histochemical test for activity of A6J /9 hydroxysteroid dehydrogenase (A5,3 B OHSD) an enzyme which is involved in progesterone synthesis (FISCHER and KAHN, 1972).…”

Section: Methodsmentioning

confidence: 99%

The Direct Influence of Stallion Semen on Progesterone Production in Cultured Corpus Luteum Cells

Gregoraszczuk

Okólski

Galas

1980

Zentralblatt für Veterinärmedizin Reihe A

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Summary Postovulatory and middle luteal phase corpora lutea of pig ovaries were cultured as monolayers with the addition of three different fractions of stallion semen. The semen was collected from an Anglo‐Arab male and the entire ejaculate was separated into three fractions from consecutive parts of the reproductive tract. The third fraction exerted a luteotropic effect on corpora lutea cells collected at the early and middle luteal phases of the cycle. These results suggest that the third seminal fraction contained PGE. The cells of both types of corpus luteum showed an increase of enzyme activity and of progesterone secretion. Zusammenfassung Direkter Einfluß von Hengstsamen auf die Progesteronsekretion von kultivierten Gelbkörperzellen Unter Zugabe von drei verschiedenen Fraktionen von Hengstsamen wurden Gelbkörperzellen von Schweinen der postovulatorischen und mittleren Lutealphase als Monolayer gezüchtet. Das Sperma wurde von einem Angloaraber‐Hengst gewonnen, und zwar in Form von drei Fraktionen aus den verschiedenen Teilen des Genitaltraktes (1. Fraktion: Nebenhodenschwanz und Samenblasen, 2. Fraktion: Cowpersche Dröse und Prostata, 3. Fraktion: restliches Sekretgemisch der akzessorischen Drüsen). Die 3. Fraktion übte auf die Gelbkörperzellen, welche aus der frühen und mittleren Lutealphase stammten, einen luteotropen Effekt aus. Die Ergebnisse lassen vermuten, daß die 3. Fraktion Prostaglandin E enthält. Beide Typen der Gelbkörperzellen wiesen eine verstärkte Enzymaktivität und Progesteronsekretion auf. Résumé Influence directe du sperme d'étalon sur la sécrétion de progestérone des cellules cultivées de corps jaune Des cellules de corps jaune de porcs de la phase de post‐ovulation et de la phase moyenne de lutéinisation ont été cultivées comme «monolayer» avec une adjonction de trois fractions différentes de sperme d'étalon. Le sperme provenait d'un étalon anglo‐arabe et sous la forme de trois fractions de parties différentes du tractus génital (Fraction 1: épididyme et vésicule séminale. Fraction 2: glande de Cowper et prostate. Fraction 3: mélange de sécretion final des glandes accessoires). La troisième fraction a exercé un effet lutéotrope sur les cellules de corps jaune qui prevenaient de la phase précoce et moyenne de lutéinisation. Les résultats permettent de supposer que la troisème fraction contient une prostaglandine E. Les deux types de cellules de corps jaune ont montré une activité enzymatique renforcée et une sécrétion de progestérone. Resumen La influencia directa del semen de caballos enteros sobre la producción de progesterona en células cultivadas de cuerpo amarillo Bajo la adición de tres fracciones diferentes de semen de caballos enteros se cultivaron células de cuerpo amarillo de cerdas de las fases luteínicas postovulatorias y medias como «monocapa». El esperma se obtuvo de un macho angloárabe, a saber en forma de tres fracciones de las partes diversas del tracto genital (1° fracción: epidídimos y vesículas seminales, 2° fracción: glándula de Cowper y próstata, 3° fracción: mezcla...

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Histochemical studies of rat ovarian follicular cells in vitro

Cited by 35 publications

References 12 publications

Lack of an inhibitory influence of oocytes upon luteinization of porcine granulosa cells in culture

Lack of an inhibitory influence of oocytes upon luteinization of porcine granulosa cells in culture

Physiology and Genetics of Reproduction

The Direct Influence of Stallion Semen on Progesterone Production in Cultured Corpus Luteum Cells

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