2023
DOI: 10.33899/ijvs.2022.133402.2222
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Histochemical study of the hepatic metacestodes in sheep infected with hydatidosis

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“…In addition, the histological changes in the affected liver have been investigated by using several tissue’s stains that hydatid cyst infection leads to a variety of histopathological changes. 48 Cyst pathogenesis, growth, and development are fundamentally influenced by immune response modulation and controlled cell death. It has been concluded that the E. granulosus cyst causes degeneration, haemorrhage, and congestion, besides fibrosis in the histological structure of the affected area.…”
Section: Discussionmentioning
confidence: 99%
“…In addition, the histological changes in the affected liver have been investigated by using several tissue’s stains that hydatid cyst infection leads to a variety of histopathological changes. 48 Cyst pathogenesis, growth, and development are fundamentally influenced by immune response modulation and controlled cell death. It has been concluded that the E. granulosus cyst causes degeneration, haemorrhage, and congestion, besides fibrosis in the histological structure of the affected area.…”
Section: Discussionmentioning
confidence: 99%
“…Samples of the stomach were fixed in neutral buffered formalin 10% for 72 h, followed by treatments with various alcohol concentrations for dehydration and xylene clearances, which were then embedded in hot paraffin wax and sectioned at a thickness of 6 microns. The tissue sections were stained using the Hematoxylin and Eosin stain (16), Periodic Acid Stain/Alsian Blue PAS/AB histochemical stain (17), and immunohistochemical staining of COX-2 expression using Rabbit monoclonal antibody against COX-2. The streptavidin-biotin enhanced system is the primary antibody, with a dilution of 1:20.…”
Section: Gross Appearance Fixation and Tissue Processingmentioning
confidence: 99%