Histochemical study on rat liver glycogen during dab carcinogenesis

Forget, Amélie; Daoust, R.

doi:10.1002/ijc.2910050315

Cited by 23 publications

(2 citation statements)

References 14 publications

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“…From the sequence of cellular changes observed during hepatocarcinogenesis in the rat liver we concluded that the clear and acidophilic glycogen storage cells persisting after withdrawal of the carcinogen represent a preneoplastic cell population, the neoplastic transformation of which is accompanied by a gradual reduction of the glycogen and a concomitant increase in ribosomes (4,10). The findings of many other authors (31,33,79,84,100 and others) and recent results of enzyme-histochemical investigations (38) are in agreement with this concept. Further support is given by observations in mice treated with various hepatocarcinogens (12,47,65,77,104) and results obtained in some other species, such as Mastomys (106) and monkeys (76).…”

Section: Sequential Cellular Changes During Hepatocarcinogenesis In Ssupporting

confidence: 81%

Biological markers of preneoplastic foci and neoplastic nodules in rodent liver

et al. 1982

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Foci of altered hepatocytes are regularly observed early during hepatocarcinogenesis in rodents. The abnormal hepatocytes may show a number of different phenotypes as characterized by various cytomorphological and cytochemical markers. The first appearance and the further development of the abnormal cell populations depend on the dose of the carcinogen given and on the duration of the carcinogenic treatment. According to cytochemical, morphometric and autoradiographic findings in rats receiving low doses (2-10% of the LD 50/kg bw/ day) of hepatocarcinogens for limited periods (iistop" experiments), glycogenotic (clear or acidophilic) hepatocytes indicate the first step of the neoplastic cell transformation which can be detected by these methods at present. The glycogenotic cells undergo a characteristic metamorphosis and give rise to basophilic tumor cells poor in glycogen, but rich in ribosomes. Under extreme experimental conditions, such as a single or repeated application of higher doses of one or several chemical carcinogens a puzzling picture emerges which is "reversible" to a large extent after withdrawal of the respective compounds. This observation points to a phenotypic instability of the cellular changes induced in certain experimental systems. Foci of altered hepatocytes persisting after withdrawal of the carcinogenic compounds are considered preneoplastic lesions. They may transform into neoplastic nodules which are also persistent and share a number of cytomorphological and cytochemical markers with the focal lesions. The persistent nodules progress to hepatocarcinomas after lag periods of weeks or months. However, the foci may also progress to hepatocarcinomas without passing a nodular intermediate stage. The development of both neoplastic nodules and carcinomas from the preneoplastic glycogen storage foci can proceed independent of further administration of carcinogen. The sequence of cellular changes during hepatocarcinogenesis derived from the experimental results in rodents is strongly supported by observations in humans, especially by the increasing reports on the appearance of hepatic tumors in patients who suffer from inborn hepatic glycogenosis.

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Section: Sequential Cellular Changes During Hepatocarcinogenesis In Ssupporting

confidence: 81%

Biological markers of preneoplastic foci and neoplastic nodules in rodent liver

et al. 1982

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“…Its content in liver tumor cells varies greatly. Glycogen diminishes during chemical hepatocarcinogenesis and is not present in most fully developed hepatocellular tumors (Bannasch and Muller, 1964;Bannasch, 1968;Edwards and White, 1942;Forget and Danoust, 1970;Laqueur et al, 1963;Schauer and Kunze, 1968;Sydow and Fry, 1969). Glycogen also diminishes in virus-induced avian hepatomas (Lapis, 1979) and human hepatomas Schaff et al, 1971), whereas it is abundant in hepatocellular carcinoma of the tubular or acinar type (Lapis and Johannessen, 1979).…”

Section: Glycogen and Glucose-6-phosphatasementioning

confidence: 99%

Correlation between growth rate and cytochemistry in morris hepatomas

1982

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The correlation between the cytochemistry (glycoprotein, glycogen, glucose-6-phosphatase, catalase, alkaline phosphatase) and the growth rate of the fast-growing Morris hepatoma 3924A and the slow-growing Morris hepatoma 9618A was studied by utracytochemical techniques. By the chromic acid-phosphotungstic acid technique, acid glycoprotein is stained in glycocalyx, Golgi saccules and vesicles, and secretory granules of the tumor cells of both hepatomas. However, the hepatoma 3924A cells contain thicker glycocalyx and more numerous glycoprotein-rich granules than hepatoma 9618A cells. Abundant alpha and beta glycogen particles are found in hepatoma 3924A. Moderate glucose-6-phosphatase activity is observed in the cisternae of endoplasmic reticulum and nuclear envelope of hepatoma 9618A, but it is totally absent in hepatoma 3924A. High catalase activity is present in numerous peroxisomes of hepatoma 9618A. Hepatoma 3924A contains only a few catalase-positive microperoxisomes. Weak to moderate alkaline phosphatase is present in the plasma membrane and nuclear envelope of hepatoma 9618A cells, while hepatoma 3924A shows no activity of the enzyme. All the cytochemical parameters except glycoprotein show an inverse relationship with the growth rate of the hepatomas. The higher intracellular glycoprotein content of hepatoma 3924A may be related to differences in cell coat secretion (composition and activity) from the slower-growing hepatoma 9618A

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