2000
DOI: 10.1016/s0891-5849(00)00395-6
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Histochemical visualization of oxidant stress

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Cited by 58 publications
(34 citation statements)
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“…The thiobarbituric acid reactive species assay was performed to assess the level of oxidized lipids in the membrane, as previously described (3). To quantify oxidized protein levels, protein carbonyl content was measured with the dinitrophenyl hydrazine assay (8).…”
Section: Methodsmentioning
confidence: 99%
“…The thiobarbituric acid reactive species assay was performed to assess the level of oxidized lipids in the membrane, as previously described (3). To quantify oxidized protein levels, protein carbonyl content was measured with the dinitrophenyl hydrazine assay (8).…”
Section: Methodsmentioning
confidence: 99%
“…level [51]. For measuring H 2 O 2 , 2′,7′-dichlorfluorescein-diacetate (DCFH-DA) is the most extensively used chemical detector, given its simple and direct chemistry with ROS [52,53]. Esterized DCFH-DA enters cells via diffusion, becomes deacetylated and converted to DCFH.…”
Section: Measurement Of Cellular Rosmentioning
confidence: 99%
“…These fluorescent techniques suffer from a lack of specificity, but have high sensitivity. Histochemical techniques for identification of oxidant stress have been described, and these techniques have recently been reviewed; 13 an example is the direct Schiff's reaction for the localization of lipid peroxidation in tissues.…”
Section: Identification Of Oxidative Damagementioning
confidence: 99%