Histochemische Untersuchungen des ?lipoiden Pigmentes? in den Ganglienzellen des Gehirns

Stammler, A.

doi:10.1007/bf00956481

Cited by 19 publications

(3 citation statements)

References 17 publications

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“…We have simply selected sudanophilia, autofluorescence, acid-fastness and positiveness to PAS and PAS-D because we consider that, for practical identification purposes, these are the most consistent properties of lipofuscin and of a variety of ceroid pigments. Although the lipid inextractability from cardiac and neuronal lipofuscin granules in situ observed in this study appears in line with numerous previous studies (Hodge 1894;Strehler 1964;Wolman 1964;Lillie 1965;Clara 1965;Gedigk and Pioch 1965;Nishioka et al 1968;Porta and Hartroft 1969), a partial attenuation of the osmiophilia and/or sudanophilia after treatment with diverse lipid solvents has been also reported (Rosin 1896;Mfihlmann 1901;Stammler 1959;Nandy 1971). The PAS reactivity of lipofuscin in situ, which was immutable to the extractive procedure used in this study and in a previous one (Elleder 1989), was found attenuated or even abolished by other investigators using similiar or different solvents (Hall et al 1989;Stammler 1959).…”

Section: Resultssupporting

confidence: 90%

Lectin histochemistry of lipofuscin and certain ceroid pigments

Monserrat

Benavides

Berra

et al. 1995

Histochem Cell Biol

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Little is known at present about the saccharide components of lipofuscin (age pigment) and ceroid pigments in situ. The purpose of this study was, therefore, to study in detail the lectin reactivities of lipofuscin in neurons and cardiac myocytes of old humans and rats. In addition, those of diverse ceroid pigments found in human aortic atheromas, in the livers of choline-deficient rats, in the uteri of vitamin E-deficient rats and in the crushed epididymal fat pad of rats, are included. Cryostat and deparaffinized sections from all these tissues were either extracted with a solvent mixture of chloroform-methanol-water (10:10:3, v/v) and incubated with 7 different biotinylated lectins or left untreated. Delipidation was done in order to study whether it was possible to discriminate between the saccharide moieties of glycolipids and glycoproteins of lipofuscin and ceroid pigments in situ. Other similarly treated sections were used to study the autofluorescence, sudanophilia, acid-fastness and reactivity to PAS. The frequency and intensity of lectin binding and standard histochemical properties of all the pigments were evaluated semi-quantitatively and blind. The results indicated that mannose was in general the most consistently detected sugar residue in lipofuscin granules of humans and rats, and that this pigment may also contain acetylglucosamine, acetylgalactosamine, sialic acid, galactose and fucose. However, notable differences were found not only in the lipofuscin saccharide components of different cell types of humans and rats, but also in those in the same type of cells in both species. Although mannose was not detected in the hepatic ceroid of choline-deficient rats, this saccharide moiety was almost always present in the other ceroid pigments. Each of the ceroids also contained other types of saccharides although the frequency of the latter varied between different ceroid pigments. While lipofuscin and each of the ceroid pigments showed somewhat different lectin binding patterns, the variability in the frequency of reactivity to lectins suggests that these patterns may not be permanent but transient. In this sense, it appears that lectin histochemistry may not allow these pigments to be differentiated. Furthermore, the extractive procedures used in this study did not enable us to determine whether the saccharides detected in the pigments in situ corresponded to glycolipids or glycoproteins.

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Section: Resultssupporting

confidence: 90%

Lectin histochemistry of lipofuscin and certain ceroid pigments

Monserrat

Benavides

Berra

et al. 1995

Histochem Cell Biol

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“…Histochcmische Untersuchungen ergaben, daß cs sich bei dem Lipofuszin nicht um eine einheitliche Substanz handelt, sondern daß es aus verschiedenen, unterschiedlich reagierenden Bestand teilen (Lipoiden, Lipoproteiden, Glukoproteiden) zusammenge setzt ist. Der Anteil und der Charakter der einzelnen Bausteine des Lipofuszins ändern sich auch im Laufe des Lebens (Stammler, 1959). In der Jugend besteht der Lipoidanteil vornehmlich aus löslichen, mit Sudan-III gut färbbaren Lipoiden, die im Alter zunehmend einem nicht cluierbaren, mit Sudan-Schwarz-B färbbaren Teil Platz machen.…”

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“…Dieser nichtlösliche Anteil der Lipoide ist derjenige, der die gelb-braune Eigenfarbc des Pigmentes bedingt, er dürfte aus oxy dierten ungesättigten Lipiden bestehen (Stammler, 1959;Gedigk und Fischer, 1959).…”

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