Histochemistry and Electron Microscopy of Islets

Boquist, Lennart

doi:10.1007/978-1-4684-2325-9_6

Cited by 8 publications

(4 citation statements)

References 232 publications

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“…Mitoses in insular cells were not found, except for the embryonic period and regeneration. Later, mitoses were assigned to B-and agranular cells (Boquist and Emdin 1985). We observed mitoses in ductal, exocrine, as well as endocrine cells, however, they were cells with fine dense granules, only.…”

Section: Discussionmentioning

confidence: 67%

Development of the Rabbit Pancreas with Particular Regard to the Argyrophilic Cells

Titlbach

Maňáková

2007

Acta Vet. Brno

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Titlbach M., E. Maňáková: Development of the Rabbit Pancreas with Particular Regard to the argyrophilic Cells. acta vet. Brno 2007, 76: 509-517.The aim of the study was the description of the prenatal development of rabbit pancreas, cell modifications, and changes in their volume and mitotic activity.immunohistochemical, light and electron microscopic procedures were employed. Stereological methods were used for estimation of cellular and nuclear volumes. hits on epithelial cells, tubular lumens, and endocrine progenitor cells were counted by systematic field sampling using test grid. Number of mitoses was registered in various cellular types after colchicine treatment. Data obtained were converted to 1 mm 3 tissue. First granules were observed in cells on day 10 and 18 hours, however two different granular types are distinguishable by electron microscopy only on day 15, when insulin and glucagon can be detected immunohistochemically. Cellular volume increased remarkably in harmony with findings of granules in serous cells. Number of epithelial cells increased also exponentially. The increase was more rapid between days 13 and 15, later it appeared exponential. value of mitotic index and length of cell cycle did not change considerably between days 15 and 24. Mitoses were observed in ductal, exocrine, as well as endocrine cells. The dividing endocrine cells were those that contained fine dense granules (progenitor cells).The sub-population of progenitor cells is able to divide, however, this source of cells appears insufficient for exponential growth. Results after colchicine treatment show the increase of cell population but the life-span and a period necessary for volume multiplication vary. Mitoses decrease in both sub-populations during the prenatal period. The progenitor cells arise probably by differentiation from the ducts, because their number increases proportionally to the main cell population.

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Section: Discussionmentioning

confidence: 67%

Development of the Rabbit Pancreas with Particular Regard to the Argyrophilic Cells

Titlbach

Maňáková

2007

Acta Vet. Brno

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“…The kidney-shaped core of the fragmented granule has been reported previously Munger and Lang, 1973). The B cell granules do not display the highly crystalloid or angular structure that is characteristic of the B cell granules of other species (Boquist, 1977;Munger, 1981), although there is a suggestion of crystallinity in some granule cores. The lack of a crystalline core has been attributed to the markedly different primary structure and amino acid composition of guinea pig insulin (Munger, 1981).…”

Section: Discussionmentioning

confidence: 94%

Immunocytochemical identification of cells containing insulin, glucagon, somatostatin, and pancreatic polypetide in the islets of langerhans of the guinea pig pancreas with light and electron microscopy

1984

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Cell types containing insulin, glucagon, somatostatin, and pancreatic polypeptide were identified in guinea pig islets with light and electron microscopic immunoperoxidase staining. Cells containing immunostainable insulin (B cells) are located throughout the islets, including the islet periphery, and contain irregularly shaped granules (350-550 nm). Granule contents are of variable opacity and are often fragmented but not crystalloid. Cells containing immunoreactive glucagon (A cells) are found in the interior of islets and contain numerous spheroid electron-opaque granules (250-350 nm). Cells containing immunoreactive somatostatin (D cells) have elongate, axonlike processes that end adjacent to islet capillaries. D cells, which are very numerous and distributed uniformly throughout the islet parenchyma, contain small spheroid granules (150-25 nm) of pale electron opacity. Cells with immunoreactive pancreatic polypeptide (F cells) are rare in islets but numerous among the exocrine parenchyma. F cells contain pale spheroid granules (100-200 nm). Morphological criteria are reliable indicators for A cells and B cells, but D cells and F cells require immunostaining for positive identification.

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“…In the B cells, this stainability is thought to be due to interaction of the dye with sulfonic acid groups engendered by the splitting of the disulfide bonds of insulin molecules induced by prior oxidation [Scott and Clayton, 1953;Boquist, 1977]. In addition, there is a good correlation between the number of aldehyde fuchsin reactive granules and the amount of insulin extractable from the pancreas [Hartroft and Wrenshall, 1955].…”

Section: Discussionmentioning

confidence: 99%

Light and Electron Microscopic Studies on the Cytological Changes in the Rat Pancreatic Islets Induced by Sodium Diethyl Dithiocarbamate (DEDTC) Administration

Nakamura¹,

Fujimori²,

Shimada³

et al. 1984

Cells Tissues Organs

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The effect of sodium diethyl dithiocarbamate (DEDTC) administration upon the structure of the pancreatic islet cells has been examined in the rat. In light microscopy, the aldehyde fuchsin stainability of the B cell granules was found to increase notably at 6 h and thereafter following the medication. In electron microscopy, the B cell granules with a porous core were shown to predominate over those with a homogeneous core containing paracrystalline structures between 30 min and 3 h following the medication. Between 6 and 12 h following medication, occasional B cells were seen to be provided with large secretory granules with a porous core. The results of the present study have been discussed with special reference to the chelating action of DEDTC.

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Histochemistry and Electron Microscopy of Islets

Cited by 8 publications

References 232 publications

Development of the Rabbit Pancreas with Particular Regard to the Argyrophilic Cells

Development of the Rabbit Pancreas with Particular Regard to the Argyrophilic Cells

Immunocytochemical identification of cells containing insulin, glucagon, somatostatin, and pancreatic polypetide in the islets of langerhans of the guinea pig pancreas with light and electron microscopy

Light and Electron Microscopic Studies on the Cytological Changes in the Rat Pancreatic Islets Induced by Sodium Diethyl Dithiocarbamate (DEDTC) Administration

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