Histochemistry of lignin and suberin deposition in boundary layers formed after wounding in various plant species and organs

Rittinger, P. A.; Biggs, Alan R.; Peirson, David R.

doi:10.1139/b87-258

Cited by 92 publications

(81 citation statements)

References 18 publications

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“…Seven days after explantation a thick layer was deposited and fading was hardly detectable. We did not find a lignification of the cells prior to suberization, as has been reported for various plant species and organs by Kolattukudy (1984) and Rittinger, Biggs & Peirson (1987).…”

Section: Ability Of Explants To Form Floral Budssupporting

confidence: 85%

Suberization and bud formation in tobacco explants

1990

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SUMMARYWound-healing and floral bud formation were studied in explants of Nicotiana tabacum L. Examination of sectioned material by conventional light microscopy, fluorescence microscopy and electron microscopy showed that suberin is formed in cells at the cut surface in close contact with the medium, after 4-7 days of culture.The number of cells depositing suberin depended on the agar concentration in the medium. In explants cultured on a medium with low agar concentration (0-2 % agar), fewer cells showed suberin deposition and more callus cells without suberin were found. Explants cultured on a medium with high agar concentration (5 % agar) formed a complete layer of cells with suberin deposition.In tobacco explants floral determination occurred within 4 days after explantation. The inability of the explants to form floral buds after this period is probably due to barrier formation by suberin

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Section: Ability Of Explants To Form Floral Budssupporting

confidence: 85%

Suberization and bud formation in tobacco explants

1990

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“…Kim et al (2004) reported that wound periderm (WP) formation is related to one of the resistant responses against C. gloeosporioides infection on chili pepper fruit. Wound periderms are formed in other plants such as peach responding to wounding and parasite invasion, which function as histological defense (Agrios, 2005;Biggs and Britton, 1988;Mullick, 1977;Rittinger et al, 1987). The wound periderm formation is a common phenomenon detectable in the wound healing processes in dicotyledons and certain monocotyledons after wounding (Esau, 1977), indicating wounding itself may induce a certain level of resistance in plants.…”

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confidence: 99%

Effect of Delayed Inoculation After Wounding on the Development of Anthracnose Disease Caused by Colletotrichum acutatum on Chili Pepper Fruit

Kim¹,

Kim²,

Kim³

et al. 2008

The Plant Pathology Journal

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Detached chili pepper fruits were inoculated with the conidial suspension of Colletotrichum acutatum JC-24 simultaneously (simultaneous inoculation, SI) and at delayed time (delayed inoculation, DI) after wounding with (delayed wound inoculation, DWI) or without additional wounding (delayed non-wound inoculation, DNI) at the inoculation time. Disease severity was significantly lowered by DNI, compared to SI. By DNI, the disease reduction rates were proportional with the length of delayed time, and greater at the high temperature range (18, 23 and 28 o C) than at the low temperature (13 o C) tested. DWI was also effective in reducing the disease severity especially at 18 o C; however, its effectiveness was lower than for DNI. In light microscopy, parenchyma cells at the wounding sites were modified structurally, initially forming new cell walls crossing cytoplasm, enlarged with multiple periclinal cell divisions, and finally layered like wound periderms. In DWI, the above structural modifications occurred, showing the restriction of the fungal invasion by the cell walls in enlarged modified cells, while no definite cellular modifications were found with proliferation of fungal hyphae in SI. Sclerenchyma-like cells with thickened cell walls were proliferated around the wounding sites, which were partially dissolved by DWI, probably leading to some disease development. All of these results suggest that the decline of the anthracnose disease in pepper fruit by the delayed inoculations may be derived from the structural modifications related to the healing processes of the previous wound inflicted on the tissues.

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“…The two ends of the tubers were removed with a knife and 1 cm diameter cores removed from the periphery of the midsection of the tuber with a No. 5 were then sectioned to 1 cm length with a set of parallel knife blades. The cores were rinsed quickly (<5 s) with sterile distilled water and placed in jars sized for the temperature gradient block described below.…”

Section: Methodsmentioning

confidence: 99%

“…This process has been referred to as suberization and results in the formation ofthe polymer suberin and deposition of associated waxes (1)(2)(3)5). The polymeric materials appear to be primarily responsible for protection against pathogen entry (4), whereas the associated waxes prevent desiccation (6)(7)(8).…”

mentioning

confidence: 99%

Deposition of Aliphatic Suberin Monomers and Associated Alkanes during Aging of Solanum tuberosum L. Tuber Tissue at Different Temperatures

Dean¹

1989

Plant Physiol.

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The effect of temperature on suberization of potato tuber tissue was measured by diffusive resistance and quantitative chemical procedures. The opfimum temperature for formation of aliphatic suberin monomers and development of resistance to water vapor conduction was 26.40C whereas alkane synthesis was optimal at 18.60C. Low temperatures (<16.60C) reduced suberin monomer production more than alkane synthesis.When plants are injured they must regenerate a wound periderm that protects them from desiccation and pathogen entry. This process has been referred to as suberization and results in the formation ofthe polymer suberin and deposition of associated waxes (1-3, 5). The polymeric materials appear to be primarily responsible for protection against pathogen entry (4), whereas the associated waxes prevent desiccation (6-8).The effect oftemperature on suberization ofwounded tissue had not been studied using quantitative techniques. The purpose of this study was to establish optimal conditions for this process, to define the temperature limits, and to determine if the three parameters, diffusive resistance, suberin deposition, and alkane deposition, were similarly affected. were then sectioned to 1 cm length with a set of parallel knife blades. The cores were rinsed quickly (<5 s) with sterile distilled water and placed in jars sized for the temperature gradient block described below. MATERIALS AND METHODSThe temperature treatments were conducted by the use of an aluminum block that was bored to receive 7 x 8.5 cm jars. The two ends of the block were hollowed out to make flowthrough reservoirs for hot and cold water to create the temperature gradient. The gradient created by the system was linear over the entire temperature range. Temperatures were maintained with a Blue M (Blue Island, IL) portable cooling unit for the cold water source and a Fisher Scientific model 730 (Fisher Scientific, Pittsburgh, PA) immersion circulator controller for the hot water source. Water was pumped to the gradient block either by the temperature controller unit or by a submersed pump in the water reservoir.Jars for sample placement contained three rings of Tygon tubing covered with a rubber mesh and an absorbent unidirectionally permeable disposable diaper (Huggies, Kimberly Clark Corp., Neenah, WI) material cut to fit the inside of the jar. Sterile water was added to the jar prior to the placement of the mesh and diaper material. Twenty-five cores approximately 1 g each were placed in each jar on top of the diaper material. This system was found to be most satisfactory for maintaining the relative humidity at near 100% while protecting the tissue from free water sources. Two jars were used for each temperature treatment.

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Histochemistry of lignin and suberin deposition in boundary layers formed after wounding in various plant species and organs

Cited by 92 publications

References 18 publications

Suberization and bud formation in tobacco explants

Suberization and bud formation in tobacco explants

Effect of Delayed Inoculation After Wounding on the Development of Anthracnose Disease Caused by Colletotrichum acutatum on Chili Pepper Fruit

Deposition of Aliphatic Suberin Monomers and Associated Alkanes during Aging of Solanum tuberosum L. Tuber Tissue at Different Temperatures

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