Histochemistry of nadph-diaphorase, a marker for neuronal nitric oxide synthase, in the peripheral autonomic nervous system of the mouse

Grozdanovic, Zarko; Baumgarten, H. G.; Brüning, Gerold

doi:10.1016/0306-4522(92)90351-2

Cited by 230 publications

(89 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Immunohistochemistry for nNOS in the mouse stomach confirmed that there is an abundance of myenteric neurons and their processes that are immunopositive for nNOS, supporting previous literature (Grozdanovic et al, 1992;Neuhuber et al, 1994;Raab and Neuhuber, 2004). We found vagal afferent fibers, traced from the nodose ganglia, formed IGLEs surrounding myenteric ganglia containing nNOS-immunopositive neurons.…”

Section: Discussionsupporting

confidence: 90%

Nitric Oxide as an Endogenous Peripheral Modulator of Visceral Sensory Neuronal Function

Page¹,

O’Donnell²,

Cooper³

et al. 2009

J. Neurosci.

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 90%

Nitric Oxide as an Endogenous Peripheral Modulator of Visceral Sensory Neuronal Function

Page¹,

O’Donnell²,

Cooper³

et al. 2009

J. Neurosci.

View full text Add to dashboard Cite

“…They are numerous in the guinea-pig (Gabella 1990) and less numerous in the cat (Feher et al 1979) ferret, rabbit (Gabella 1990), horse (Prieto et al 1989) and female pig (Pidsudko 2004). No, or very few, UB-IG have been found in the mouse (Grozdanovic et al 1992) and rat (Gabella and Uvelius 1990).…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical characteristics and distribution of neurons in the intramural ganglia supplying the urinary bladder in the male pig

Pidsudko

2013

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

This study investigated the distribution and chemical coding of neurons in intramural ganglia of the urinary bladder trigone (UBT-IG) and cervix (UBC-IG) in the male pig using combined retrograde tracing and double-labelling immunohistochemistry. Additionally, immunoblotting was used to confirm the presence of marker enzymes for main populations of autonomic neurons. Retrograde fluorescent tracer Fast Blue (FB) was injected into the wall of both the left and right side of the bladder trigone, cervix and apex during laparotomy performed under thiopental anaesthesia. Twelve μm-thick cryostat sections were processed for double-labelling immunofluorescence with antibodies against tyrosine hydroxylase (TH), dopamine β-hydroxylase (DBH), neuropeptide Y (NPY), somatostatin (SOM), galanin (GAL), vasoactive intestinal polypeptide (VIP), nitric oxide synthase (NOS), calcitonin gene-related peptide (CGRP), substance P (SP) and vesicular acetylcholine transporter (VAChT). UBT-IG and UBC-IG neurons in both parts of the organ formed characteristic clusters (from few to tens of neuronal cells) found under visceral peritoneum or in the outer muscular layer. Immunohistochemistry revealed several subpopulations in UBT-IG and UBC-IG neurons, namely noradrenergic (ca. 76% and 76%), cholinergic (ca. 22% and 20%), non-adrenergic/non-cholinergic nerve cells (ca. 1.5% and 3.8%), NPY-(ca. 66% and 58%), SOM-(ca. 39% and 39 %), VIP-(ca. 5% and 0%) and NOS-immunoreactive (IR) (ca. 1.5% and 3.8%), respectively. Immunoblotting using antibodies to TH and VAChT showed the presence of studied proteins as revealed by the presence of protein bands of the correct molecular weight. This study has revealed a relatively large population of differently coded UBT-and UBC-IG neurons, which constitute an important element of the complex neuroendocrine system involved in the regulation of the male urogenital organs function.

show abstract

“…These were also lost after 72 h of cold-storage and the NOS activity of the tissue, measured biochemically, was reduced by 97%. Since NOS appears to be located mainly within nerves in peripheral organs (apart from the vascular endothelium ;Bredt et al, 1990;Mitchell et al, 1991;Grozdanovic et al, 1992) (Waldman & Murad, 1987) and therefore one explanation maybe that the activity of this enzyme is increased following cold storage. Since the S-nitrosothiols appeared to act directly on the smooth muscle to produce relaxation, it was important to investigate the effect of drugs known to influence the direct effects of nitrovasodilators.…”

Section: Discussionmentioning

confidence: 99%

An investigation of some S‐nitrosothiols, and of hydroxy‐arginine, on the mouse anococcygeus

Gibson

Babbedge

Brave

et al. 1992

British J Pharmacology

View full text Add to dashboard Cite

1 The effect of five S-nitrosothiols, and of the stereoisomers of N0-hydroxy-arginine (HOARG), were investigated on the mouse anococcygeus. 2 All five S-nitrosothiols produced concentration-related (0.1-100IM) relaxations of carbachol (50 LM)-induced tone; the order of potency was S-nitroso-L-cysteine (CYSNO)> S-nitroso-N-acetyl-D,Lpenicillamine (SNAP)>S-nitrosoglutathione (GSNO)> S-nitrosocoenzyme A (CoASNO)> S-nitroso-N-acetyl-L-cysteine (NACNO). The relaxations were unaffected by the nitric oxide synthase (NOS) inhibitor, L-NG-nitro-arginine (10 JIM) (L-NOARG).3 Cold-storage of the tissue for 72 h resulted in loss of sympathetic and non-adrenergic, noncholinergic (NANC) nerve function. NOS activity in the tissue was reduced by 97%. Despite this, relaxations induced by the S-nitrosothiols were unaffected. 4 Haemoglobin (50 jM) attenuated relaxations induced by NO and the S-nitrosothiols, although responses to 3-isobutyl-1-methyl-xanthine were unaffected. N-methyl-hydroxylamine (2 mM) which has been shown previously to produce selective inhibition of NANC and nitrovasodilator responses in this tissue, also reduced responses to all S-nitrosothiols.5 Hydroquinone (100 gM) greatly reduced relaxations to CYSNO (by 88%) but had no effect on those to SNAP, GSNO, CoASNO or NACNO. Since hydroquinone does not reduce responses to NANC stimulation, CYSNO is unlikely to be the NANC transmitter. 6 L-HOARG by itself (up to 100 piM) had no significant effect on carbachol-induced tone or on NANC (10 Hz; 10 s train every 100 s) relaxations. However, it produced reversal of the inhibitory effects of L-NOARG (10;pM), being only slightly less potent than L-arginine. D-HOARG was without effect. L-HOARG had no effect on relaxations induced by 1.51iM NO. 7 The results show that S-nitrosothiols are potent relaxants of the mouse anococcygeus; they act directly on the smooth muscle with a mechanism similar to NO and other nitrovasodilators. In addition, the results are consistent with L-HOARG being an intermediate in the biosynthesis of NO from L-arginine, although there is no evidence for it acting to stabilize NO extracellularly.

show abstract

Histochemistry of nadph-diaphorase, a marker for neuronal nitric oxide synthase, in the peripheral autonomic nervous system of the mouse

Cited by 230 publications

References 35 publications

Nitric Oxide as an Endogenous Peripheral Modulator of Visceral Sensory Neuronal Function

Nitric Oxide as an Endogenous Peripheral Modulator of Visceral Sensory Neuronal Function

Immunohistochemical characteristics and distribution of neurons in the intramural ganglia supplying the urinary bladder in the male pig

An investigation of some S‐nitrosothiols, and of hydroxy‐arginine, on the mouse anococcygeus

Contact Info

Product

Resources

About