Histochemistry of the zona pellucida of the ovary of a species with natural polyovulation: <i>Lagostomus maximus</i> (Rodentia, Hystricomorpha, Chinchillidae)

Acuña, Francisco; Hoz, María Florencia Tano de la; Díaz, Alcira Ofélia; Portiansky, Enrique Leo; Barbeito, Claudio Gustavo; Flamini, Mirta Alicia

doi:10.1111/rda.13333

Cited by 10 publications

(13 citation statements)

References 67 publications

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“…These data indicate the presence of GlcNAc and β‐ d ‐galactose in these ovarian components of sows. Similar findings were noted in the zona pellucida of prepubertal and adult pigs (Pastor et al, ), viscacha (Acuña et al, ) and wild ungulates (Parillo, Dallâ€™Aglio, Verini Supplizi, Ceccarelli, & Gargiulo, ). In addition, UEA‐1 presented weak or absent reaction in the zona pellucida in the present investigation, whereas other researchers detected mild (Parillo et al, ) or moderate (Pastor et al, ) marcation in the porcine zona pellucida.…”

Section: Discussionsupporting

confidence: 78%

“…Lectin histochemistry (LHC) is an important technique effectively used in the identification and in situ characterization of carbohydrates in ovarian follicles of many species, such as rat, mare, pig, viscacha ( Lagostomus maximus ) and women (Acuña et al, ; Barbeito et al, ; Desantis et al, ; Maymon et al, ; Pastor et al, ; Salvetti et al, ). This technique was also used to identify glycan residues in equine and porcine cumulus–oocyte complexes in in vitro ‐ and in vivo‐matured samples (Accogli et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Lectin histochemical pattern on the normal and cystic ovaries of sows

Mendes

Pinto

Gimeno

et al. 2019

Reprod Domestic Animals

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The lectin histochemical pattern (LHP) was characterized and compared in normal and cystic ovaries of sows. Six biotinylated lectins (PNA, SBA, WGA, RCA‐1, DBA and UEA‐1) were used on tissue sections. In the normal ovaries, the reaction to UEA‐1 and SBA was mild to moderate in mesothelial and endothelial cells. RCA‐1 staining was mild to moderate in theca interna of growing follicles, corpora luteum and mesothelium. In addition, this lectin presented strong reaction in endothelial cells, granulosa cells of atretic follicles, zona pellucida of growing follicles and plasma. DBA showed strong intensity in mesothelial and endothelial cells. There was mild to moderate reactivity to WGA in granulosa cells, corpus luteum and theca interna of follicles in development, and moderate in zona pellucida, in granulosa cells of atretic follicles and mesothelium. PNA staining was mild to moderate in oocytes and in the adventitia and media of medullary arteries. Changes in the LHP of the cystic ovaries were noted; however, there were no differences in these findings between the follicular and luteinized cysts. UEA‐1 reactivity in the cystic ovaries was moderately reduced in the mesothelial and endothelial cells, whereas there was mild reduction in the DBA staining in the granulosa cells. Reaction to RCA‐1 and WGA in the cysts also was decreased in theca interna, zona pellucida and granulosa cells of atretic follicles. Furthermore, endothelium and theca interna in the cystic ovaries presented mild reduction of marcation to SBA, whereas there was decreased reactivity to PNA in the oocytes and adventitia and media layers of the medullary arteries. The results of the current study show that cysts modify the LHP in swine ovaries. These changes of glycoconjugates in many ovarian structures could modify diverse process and may be one of the reasons for decreased fertility in sows.

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Lectin histochemical pattern on the normal and cystic ovaries of sows

Mendes

Pinto

Gimeno

et al. 2019

Reprod Domestic Animals

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“…Moreover, the WGA, DBA, SBA, RCA‐I and PNA lectins identified the glycosidic residues GlcNac and β‐galactose, and CON‐A identified mannose and glucose residues. In mammals, these residues have an important role in the oocyte‐sperm interaction (Acuña et al ., 2018; Amari et al ., 2001; Barbeito et al ., 2013; Maegawa et al ., 2002; Yonezawa et al ., 2005). However, in Chondrichthyes, fertilization occurs in the anterior oviduct (McMillan, 2007), so the same function is not possible.…”

Section: Discussionmentioning

confidence: 99%

“…For the identification of specific sugar residues (Goldestein & Hayes, 1978) in OG, a battery of seven biotinylated lectins was used (Table 1). For this, a standardized protocol from our laboratory was used (Acuña et al ., 2018). As negative controls, slices of oviductal gland incubated only with phosphate buffered saline were used.…”

Section: Methodsmentioning

confidence: 99%

“…The intensities of the different lectins were classified according to the following semi‐quantitative scale: 0, unlabelled; 1, light stain; 2, moderate stain; and 3, intense stain, according to the criterion used in previous studies (Acuña et al ., 2018; Tano de la Hoz et al ., 2016, 2017). The evaluation of the intensity of each reaction was based on estimates from three trained independent researches, blinded to the samples being observed.…”

Section: Methodsmentioning

confidence: 99%

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Glycan expression as a tool for a deeper understanding of a reproductive gland in a skate of economic importance

Moya

Acuña

Andrade

et al. 2020

Journal of Fish Biology

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Atlantoraja platana is an oviparous skate endemic to the south‐west Atlantic Ocean, and is one of the skate species most exploited by local industrial bottom trawl fisheries. Oviparous elasmobranchs encapsulate their eggs in complex egg cases produced by the oviductal gland (OG). This organ is exclusively present in these fishes and comprises four distinct zones: club, baffle, papillary and terminal. The relative size and structural complexity of these zones correlate with mode of reproduction. Glycans are known to play major roles in reproduction so their distribution in each zone of the OG could explain the functional multiplicity of the gland in skates, but this topic has not been previously investigated. In this study, morphological, histochemical and lectin‐histochemical analysis revealed various novel aspects of A. platana's OG. The club, papillary and terminal zones positively stained for periodic acid Schiff's reagent (PAS) and Alcian Blue (AB), indicating the presence of neutral and acid mucopolysaccharides. However, the buffle zone was negative for PAS and AB stains, but was positive for all the lectins used. Each zone of the OG had a characteristic pattern of glycan expression. Finally, we confirmed the presence of sperm but not sperm storage. This is the first lectin‐histochemical study of the OG in chondrichtyan fish and it has proven to be an important tool to understand some of the mechanisms of fertility and reproductive success in economic important species such as A. platana.

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Early and natural embryonic death in Lagostomus maximus: Association with the uterine glands, vasculature, and musculature

Acuña

Barbeito

Portiansky

et al. 2020

Journal of Morphology

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The uterus is an organ with great plasticity due to the morphological and physiological changes it experiences during the estrous cycle and pregnancy. In mammals, pregnancy requires diverse sex hormones, growth factors and cytokines, among others, for promoting uterine remodeling to favor implantation, placentation, and embryo/fetus survival and growth. The hystricognathi rodent Lagostomus maximus (plains viscacha) has a high rate of embryonic resorption. The cranial and middle implants are reabsorbed 25–35 days after intercourse while the caudal embryos continue with their development until two precocial offspring are born. So far, no uterine studies of non‐pregnant L. maximus females were performed to determine the possible existence of variations in the organ that could be related to the differential survival of the implants. We used ultrasonography, as well as morphological, morphometric, histochemical, lectinhistochemical, and immunohistochemical methods to study differences in the uterine glands (area), vasculature (area), and musculature (thickness) along the uterine horns in non‐pregnant females. Along the uterus, all these structures were in more advanced developmental condition in the caudal region as compared to more anterior positions. These regional variations could be decisive in explaining the reason why only caudal implantations come to term. In contrast, no differences in the in the luminal and glandular epithelial cells, nor in the degree of cell proliferation and apoptosis, and hormonal receptor staining were found. These parameters could be related to implantation along the uterine horns, but not to the differential survival of the implants.

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Histochemistry of the zona pellucida of the ovary of a species with natural polyovulation: Lagostomus maximus (Rodentia, Hystricomorpha, Chinchillidae)

Cited by 10 publications

References 67 publications

Lectin histochemical pattern on the normal and cystic ovaries of sows

Lectin histochemical pattern on the normal and cystic ovaries of sows

Glycan expression as a tool for a deeper understanding of a reproductive gland in a skate of economic importance

Early and natural embryonic death in Lagostomus maximus: Association with the uterine glands, vasculature, and musculature

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