Histochemistry. V. Soyasaponins in soybeans (Glycine max MERRILL, seeds).

Tani, Tadato; Katsuki, Tadahisa; Kubo, Michinori; Arichi, Shigeru; Kitagawa, Isao

doi:10.1248/cpb.33.3829

Cited by 22 publications

(17 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The soyasaponin content was reported to be very high in soybean seed hypocotyls 41,42) and pea seedlings. 43) These results suggest that the induction of soyasaponin biosynthesis during germination is common in these legumes.…”

Section: Discussionmentioning

confidence: 99%

Differential Expression of Three Oxidosqualene Cyclase mRNAs in Glycyrrhiza glabra

Hayashi

Huang

Takada

et al. 2004

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Higher plants produce not only sterols but also various triterpenes, which are derived from six isoprene units, 1) and some triterpenes are further glycosylated to produce various triterpene saponins.2) These triterpenes and triterpene saponins are believed to participate in the plant defence systems against microbial pathogens or insects. [3][4][5] An interesting effect that has also been reported is that an exogenous triterpene saponin stimulates the growth of roots in several plants. 6,7) In addition to the benefit for plants themselves, these triterpenes and saponins are beneficial for human beings as medicines, sweeteners, detergents, and cosmetics. Although structural elucidation of triterpenes and saponins has been extensively studied, 1,2) our understanding about the regulation of their biosyntheses is quite limited at the molecular level. 8)Oxidosqualene cyclases (OSCs) catalyze the cyclization of 2,3-oxidosqualene, a common intermediate of both sterols and triterpenes. Lanosterol synthase, playing a crucial role in cholesterol biosynthesis, is a unique OSC in mammals. In higher plants, on the other hand, some OSCs responsible for triterpene biosynthesis co-exist with cycloartenol synthase corresponding to lanosterol synthase in mammals. 8,9) Up to now, cDNAs of OSCs including cycloartenol synthase, bamyrin synthase, lupeol synthase and multifunctional triterpene synthase have been functionally characterized from various plant species. 8) After cloning of these OSC genes, our interest has shifted the regulatory roles of these OSCs in triterpene and saponin biosyntheses in higher plants. 8,[10][11][12][13] In our study, licorice (Glycyrrhiza glabra L., Fabaceae) was used as a model plant to elucidate the regulation of triterpene biosyntheses in higher plants (Fig. 1). Cultured licorice cells lacked the ability to produce glycyrrhizin, a sweet oleanane-type triterpene saponin produced in the roots of the mother plant, but produced phytosterols and two structurally different triterpenoid constituents, soyasaponins and betulinic acid. 15,16) Glycyrrhizin is localized exclusively in the woody parts of the thickened roots and stolons, whereas soyasaponins, oleanane-type triterpene saponins, are localized mainly in the seeds and rootlets of licorice.17) On the other hand, the content of betulinic acid, a lupane-type triterpene, is high in the cork layer of thickened licorice roots. 15) cDNAs of cycloartenol synthase, an OSC involved in sterol biosynthesis, and b-amyrin synthase, an OSC involved in both glycyrrhizin and soyasaponin biosynthesis, have been cloned from G. glabra. 18,19) In addition to these two OSCs, the third OSC, lupeol synthase, which is responsible for the biosynthesis of betulinic acid, most likely exists in cultured licorice cells. In the present study, an OSC cDNA for lupeol synthase was isolated by heterologous hybridization with that of olive lupeol synthase, 20) and the patterns of mRNA expression of the three OSCs in the cultured cells and the intact plants of G. glabra were examined. ...

show abstract

Section: Discussionmentioning

confidence: 99%

Differential Expression of Three Oxidosqualene Cyclase mRNAs in Glycyrrhiza glabra

Hayashi

Huang

Takada

et al. 2004

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…A modified method of Tani et al (1985) was used. Dehulled soybeans were ground to a fine powder and extracted with hexane in a Soxhlet extractor overnight.…”

Section: Isolation Of the Saponin Fraction From Whole Soybeansmentioning

confidence: 99%

Analysis and quantitative determination of group B saponins in processed soybean products

Berhow

Cantrell

Duval

et al. 2002

Phytochemical Analysis

View full text Add to dashboard Cite

Pure analytical standards for the major saponins present in processed soy products, the group B saponins (soyasaponins I, II, III and IV), were isolated in mg quantities by a combination of processing, precipitation/re-solubilisation, TLC and preparative HPLC. These standards were determined to be pure by LC-ESI/MS analysis and NMR. The standards were used to perfect a facile analytical HPLC method using spectrometric detection to determine the percent composition of the group B soyasaponins in various products from processing of soybean.

show abstract

“…; Tani et al. ), soyasaponin A1 and soyasaponin I were employed as the representatives of group A and group B soyasaponins, respectively.…”

Section: Methodsmentioning

confidence: 99%

The content of soyasaponin and soyasapogenol in soy foods and their estimated intake in the Japanese

Kamo¹,

Suzuki²,

Sato³

2014

Food Science & Nutrition

View full text Add to dashboard Cite

Soyasaponins have been reported to promote various health functions. However, the total soyasaponin and soyasapogenol content in soy products and the daily intake remain to be fully elucidated. We developed a high-performance liquid chromatography coupled with tandem mass spectrometric (HPLC–MS/MS) method to evaluate the content of group A and B soyasaponins and soyasapogenols. The total soyasaponin content was measured after pretreatment converted soyasaponins to soyasapogenols. The total soyasaponin content in soy foods was 200–1800 nmol g−1, although that of soy sauce was 2–7 nmol g−1. The soyasapogenol to total soyasaponin ratio was 30–50% in long-term matured miso. The majority of the soyasapogenol detected was soyasapogenol B rather than soyasapogenol A, resulting in speculation that further steps are required to liberate aglycones from glycoside-conjugated soyasaponins in soyasapogenol A. We estimated the daily intake of total soyasaponins and soyasapogenols by the Japanese, which was 50.3 and 0.59 μmol, respectively. The soyasapogenol content and the soyasapogenol to total soyasaponin ratio was considerably low in most soy products, except for long-term maturated miso. The major source of the daily intake of soyasaponins and soyasapogenols were tofu and miso, respectively.

show abstract

Histochemistry. V. Soyasaponins in soybeans (Glycine max MERRILL, seeds).

Cited by 22 publications

References 0 publications

Differential Expression of Three Oxidosqualene Cyclase mRNAs in Glycyrrhiza glabra

Differential Expression of Three Oxidosqualene Cyclase mRNAs in Glycyrrhiza glabra

Analysis and quantitative determination of group B saponins in processed soybean products

The content of soyasaponin and soyasapogenol in soy foods and their estimated intake in the Japanese

Contact Info

Product

Resources

About