Histocytological evaluation of the reaction of rat pulp tissue to Carisolv

Dammaschke, Till; Stratmann, U.; Mokrys, Kai; Kaup, Magdalena; Ott, K.

doi:10.1016/s0300-5712(01)00015-x

Cited by 24 publications

(30 citation statements)

References 17 publications

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“…However, previous research has shown that the original Carisolv caused no, or only minor, deviations to pulp tissue compared to control groups after direct [4][5][6] or indirect 7 contact. Although the effects on pulp tissue in direct contact with the original Carisolv did not differ from the controls, [4][5][6] it is still necessary to examine possible reactions of pulp tissue in direct contact with Carisolv 'new gel' in order to exclude local toxic effects. Before applying Carisolv 'new gel' to deep cavities it must be ensured that there is no enhanced risk to vital pulp tissue.…”

Section: Introductionmentioning

confidence: 80%

“…The penetration is controlled by the buffer capacity of the pulp fluid and by the magnitude of consumption of radicals during hydrolytic reactions in the tissue. 4 Although microbial analysis is usually undertaken by using specific stains such as Brown and Brenn or Brown and Hopps performed on paraffin sections, toluidine blue staining of Epon sections was used in the present study. This was done because pilot studies revealed that the bacteria detection rate in semi-thin sections (thickness 1µm) after toluidine blue staining was clearly superior to the one in paraffin sections due to superposition of image planes lying out of the focus in paraffin sections with a thickness of 8µm.…”

Section: Discussionmentioning

confidence: 99%

“…In a previous experiment 4 the perfusion fixation technique was validated to be equal to immersion fixation technique concerning ultrastructural quality of the pulpal tissue. No noticeable differences in comparing the results of both techniques could be detected.…”

Section: Histological Assessmentmentioning

confidence: 99%

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Reaction of rat pulp tissue to Carisolv ‘new gel’—‐A histocytological evaluation

Dammaschke

Stratmann

Danesh

et al. 2006

Australian Dental Journal

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Background: This study was an histological examination of pulp tissue exposed to Carisolv 'new gel' after 1 to 28 days. Methods: An occlusal cavity was prepared in 64 caries-free molar teeth of 16 Wistar rats. The roofs of the pulp chambers were perforated and Carisolv 'new gel' solution was placed onto the exposed pulps of 32 molar teeth for 20 minutes. Thirty-two contralateral molar teeth served as controls and were coated with an inert liquid containing isotonic saline solution and carmellose for 20 minutes as well. The pulps of all teeth were capped with Ca(OH) 2 and the cavities were filled with a flowable composite in combination with a self-etching dentine adhesive. The animals were sacrificed after 1, 3, 7 and 28 days. Eight teeth per group and the time period were histologically examined, scored, and statistically evaluated (Wilcoxon-test). Results:The results showed no statistically significant differences between the Carisolv group and the control group (p>0.05). The observed pulp reaction was essentially the same as those reported in the past being typical for the effect of calcium hydroxide as a direct pulp capping agent. Conclusion: Compared to Ca(OH) 2 , Carisolv 'new gel' did not cause any different or additional pulp reaction in healthy teeth.Key words: Carisolv, 'new gel', rat, pulp, tissue reaction.Abbreviations and acronyms: EDTA = ethylenediaminetetraacetic acid; LM = light microscopical; NaOC1 = sodium hypochlorite; RAPMB = Resulcin AquaPrime + MonoBond; TEM = transmission electron microscopical.

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Section: Introductionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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Reaction of rat pulp tissue to Carisolv ‘new gel’—‐A histocytological evaluation

Dammaschke

Stratmann

Danesh

et al. 2006

Australian Dental Journal

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“…[30][31][32][33] All have reported that Carisolv gel has no adverse effects on the dental pulp and interestingly, some mentioned that Carisolv has a similar action as Ca(OH) 2 pulp capping materials. 30,33 Moreover, it was reported that Carisolv has bactericidal and haemostatic effects on exposed pulp tissue, which is attributed to the alkaline pH of Carisolv of 11.…”

Section: Carisolv Instrumentsmentioning

confidence: 99%

“…30,33 Furthermore, histological findings showed Carisolv caused minimal superficial necrosis at pulp exposure sites, an effect which could promote the repair function of the injured pulp. 30,31 Only Bulut et al 33 used human pulp tissue; the teeth used were maxillary and mandibular premolars planned to be extracted for orthodontic purposes. The rationale of using human dental pulp tissue was because the repair capacity of rat pulp tissue, due to its continuous growth, is higher than that of human pulp tissue.…”

Section: Carisolv Instrumentsmentioning

confidence: 99%

Current update of chemomechanical caries removal methods

2014

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Chemomechanical caries removal is an excellent method for minimally invasive caries excavation, and the removal agents are either sodium hypochlorite (NaOCl)-or enzyme-based. The NaOCl-based agents include GK-101, GK-101E (Caridex) and Carisolv, and the enzyme-based agents include Papacarie and the experimental material, Biosolv. This review outlines the changes in chemomechanical caries removal methods and focuses on recently published laboratory and clinical studies. The historical development, mechanism of action, excavation time and biological effects on pulp and dental hard tissues are described. Based on existing evidence, the currently available chemomechanical caries removal methods are viable alternatives to conventional rotary instrument methods. Chemomechanical methods could be extremely useful in very anxious, disabled and paediatric patients. It does seem some of these agents would still benefit from quicker excavation times in order to achieve more universal acceptance. However, as a means of conserving the caries-affected dentine, chemomechanical caries removal is possibly much more successful than conventional rotary instrumentation.Keywords: Biosolv, Caridex, Carisolv, chemomechanical caries removal, minimal intervention dentistry, Papacarie.Abbreviations and acronyms: EDTA = ethylene diamine tetra-acetic acid; FDA = US Food and Drug Administration; NaOC1 = sodium hypochlorite; VAV = Visual Analogue and Verbal scale; WBFP = Wong Baker Faces Pain scale.

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Evaluation of the chemomechanical removal of dentine caries in vivo with a new modified Carisolv gel

Fure

Lingström

2004

Clin Oral Invest

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Carisolv is a minimally invasive method for softening and removing dentine caries. A new, modified Carisolv gel has been developed in order to optimise the efficiency if its chemical caries dissolution. The aim of the present study was to compare the caries removal efficiency of the original gel with that of the new gel, which contains almost double the concentration of sodium hypochlorite. Ten dentists treated 202 cavities in 170 patients; 104 cavities were randomised to the new gel and 98 to the original gel. Their mean treatment times for caries removal were 6.7+/-4.1 min and 7.6+/-4.2 min, respectively ( P>0.05). In close-to-pulp lesions, constituting 32% of the cavities, the mean times for caries removal were 9.0+/-7.0 min and 11.6+/-4.4 min for the new and original gels, respectively ( P<0.01). Questionnaires revealed that 81% of the patients preferred chemomechanical treatment to drilling. In conclusion, the improved efficiency of the modified Carisolv gel did reduce the time for caries removal in deep lesions. However, it still needs more time than conventional drilling.

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Histocytological evaluation of the reaction of rat pulp tissue to Carisolv

Cited by 24 publications

References 17 publications

Reaction of rat pulp tissue to Carisolv ‘new gel’—‐A histocytological evaluation

Reaction of rat pulp tissue to Carisolv ‘new gel’—‐A histocytological evaluation

Current update of chemomechanical caries removal methods

Evaluation of the chemomechanical removal of dentine caries in vivo with a new modified Carisolv gel

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