Histologic analysis of the implanted cartilage in an exact-fit osteochondral transplantation model

Makino, Takeshi; Fujioka, Hiroyuki; Kurosaka, Masahiro; Matsui, Noriko; Yoshihara, Hisahiro; Tsunoda, Masaya; Mizuno, Kosaku

doi:10.1053/jars.2001.24705

Cited by 64 publications

(65 citation statements)

References 11 publications

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“…cartilage surface after the 12 th week, which is then almost as normal". Makino et al [6] reported full embedding of the autograft in the rabbit model 24 wk post-operatively; an observation which comes in agreement with our findings.…”

Section: Histological/immunohistochemical Evaluation and Statistical supporting

confidence: 93%

“…In the 1950's, the first attempts were made to restore articular cartilage using skin. Even though since then, much progress has been made [1][2][3][4][5][6] , there is still lacking evidence in how to best manage these lesions. The avascular and denervated environment, the immobility of chondrocytes and the limited ability of mature chondrocytes to proliferate, are considered as the main reasons that obstruct intrinsic articular regeneration [7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

“…The current treatments include: (1) palliative (debridement), marrow stimulating techniques (microfractures, drilling, abrasion) and transfer of osteochondral unit (autograft or allograft) for lesions up to 2.5 cm 2 ; and (2) for lesions bigger than 2.5 cm 2 , use of chondrogenic potential cells (autologous chondrocyte implantation) and osteochondral allografts [10] . Increasing interest appears in one-time surgery techniques as they provide shorter recovery time, with the transfer of an entire osteochondral autograft being the most appealing [2][3][4][5][6]9,11] . Autografting represents a reasonable solution for osteochondral defects.…”

Section: Introductionmentioning

confidence: 99%

“…No consensus exists regarding the best approach to achieve optimal results [4][5][6]9,12] . The donor site, could be a major source of pain.…”

Section: Introductionmentioning

confidence: 99%

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Donor’s site evaluation after restoration with autografts or synthetic plugs in rabbits

Intzoglou

Mastrokalos

Korres

et al. 2014

WJO

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AIM:To investigate donor site's area histological and immunohistochemical knee cartilage appearances after resurfacing iatrogenic defects with biosynthetic plugs orautografts. METHODS:Thirty New Zealand White rabbits were used in this study. A full-thickness cylindrical defect of 4.5 mm (diameter) × 7 mm (depth) was created with a hand drill in the femoral groove of every animal. In Group A (n = 10) the defect of the donor site was repaired with a biosynthetic osteochondral plug, in Group B (n = 10) with an osteochondral autograft, while in Group C (control group of 10) rabbits were left untreated. RESULTS:Twenty-four weeks postoperatively, smooth articular cartilage was found macroscopically in some trocleas' surfaces; in all others, an articular surface with discontinuities was observed. Twenty-eight out of 30 animals were found with predominantly viable chondrocytes leaving the remaining two -which were found only in the control group-with partially viable chondrocytes. However, histology revealed many statistical differences between the groups as far as the International Cartilage Repair Society (ICRS) categories are concerned. Immunofluoresence also revealed the presence of collagen Ⅱ in all specimens of Group B, whereas in Group A collagen Ⅱ was found in less specimens. In Group C collagen Ⅱwas not found. CONCLUSION:The matrix, cell distribution, subchondral bone and cartilage mineralization ICRS categories showed statistically differences between the three groups. Group A was second, while group B received the best scores; the control group got the worst ICRS scores in these categories. So, the donor site area, when repairing osteochondral lesions with autografting systems, is better amended with osteochondral autograft rather than bone graft substitute implant.

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Section: Histological/immunohistochemical Evaluation and Statistical supporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…No consensus exists regarding the best approach to achieve optimal results [4][5][6]9,12] . The donor site, could be a major source of pain.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Donor’s site evaluation after restoration with autografts or synthetic plugs in rabbits

Intzoglou

Mastrokalos

Korres

et al. 2014

WJO

View full text Add to dashboard Cite

show abstract

“…The transplantation of chondrocyte grafts goes back to treatment of osteochondral lesions, particularly the use of mosaic arthroplasty. The method formerly referred to as promising, gradually became routine [11][12][13] . Foster et al was probably the first to describe the method of transplanting chondrocytes in a defect in the growth plate in 1990.…”

Section: Discussionmentioning

confidence: 99%

Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs

Plánka¹,

Srnec²,

Raušer³

et al. 2012

BIOMED PAP

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Introduction. This study describes the results achieved using a combination of allogeneic mesenchymal stem cells (MSCs) with chondrocytes (CHC) and a new scaffold consisting of type-I collagen and chitosan nanofibers in the prevention of partial growth plate arrest after iatrogenic injury in pigs. Material and methods. The miniature pig was selected as an experimental model to compare the results in the left femoral bones (MSCs and CHC in scaffold transplantation into the iatrogenic partial distal growth plate defect) and right femoral bones (scaffold alone transplantation). The experimental group consisted of 10 animals. Bone marrow from os ilium as the source of MSCs was used. A porous cylinder consisting of 0.5% by weight type-I collagen and 30% by weight chitosan, was the optimal choice. The length of the bone and angular deformity of distal femur after the healing period was measured and the quality and structure of the newly formed cartilage was histologically examined. Results. Transplantation of the composite scaffold in combination with MSCs and chondrocytes led to the prevention of growth disorder and angular deformity in the distal epiphysis of the left femur. Compared to the right (control) femur, tissue similar to hyaline cartilage with signs of columnar organization typical of the growth plate occurred in most cases. Conclusions. The promising results of this study reveal the new and effective means for the prevention of bone bridge formation after growth plate injury.

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Arthroscopic debridement for knee osteoarthritis

Laupattarakasem

Laopaiboon

Laupattarakasem

et al. 2008

Cochrane Database of Systematic Reviews

128

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Histologic analysis of the implanted cartilage in an exact-fit osteochondral transplantation model

Cited by 64 publications

References 11 publications

Donor’s site evaluation after restoration with autografts or synthetic plugs in rabbits

Donor’s site evaluation after restoration with autografts or synthetic plugs in rabbits

Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs

Arthroscopic debridement for knee osteoarthritis

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