Histologic and clinical evaluation for maxillary sinus augmentation using macroporous biphasic calcium phosphate in human

Lee, Ji Hyun; Jung, Ui Won; Kim, Chang Sung; Choi, Seong‐Ho; Cho, Kyoo-Sung

doi:10.1111/j.1600-0501.2008.01520.x

Cited by 45 publications

(36 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These finding differ from what has been presented as ideal bone regeneration processes, where according to advancement of osteogenesis (in bone regeneration induced by biomaterials), the matrix of fibrillar tissue regresses; formation of bone type I is completed by the third or fourth week, and begins the phase of progressive substitution toward type II bone, where osteoclasts and osteoblasts appear (Anitua & Andia, 2000). At the same time, it also differs with the results presented by other research (Galindo-Moreno et al; Suba et al;Friedmann et al;Gotz et al, 2008;Lee et al, 2008;Norton et al, 2003, who have found that bone genesis produces starting with osteoblasts.…”

Section: Discussioncontrasting

confidence: 56%

Histological Evaluation of Bone Regeneration Means Freeze Dried Bone Allograft (FDBA) in Post Exodontia Sockets

et al. 2011

View full text Add to dashboard Cite

SUMMARY:Within oral rehabilitation alveolar ridge preservation following extraction is important. This research study shows a histological, histochemical and histomorphometrical evaluation in two cases of post extraction ridge-socket preservation performed with FDBA. In two patients dental extraction procedures were performed and sockets were immediately filled with FDBA. Six months later a biopsy of grafted area was obtained and rehabilitated through dental implant. Grafted bone samples were treated for histological and histochemical analysis. Bone tissue area was measured. Laboratory analysis of three samples showed inactive bone surfaces, neither osteoblasts nor osteoclasts were found, only osteocyte and osteogenous cells were observed. These findings do not mean that tissue is metabolically inactive, rather bone genesis develop from a tissue matrix with the potential to generate undifferentiated osteocytes, and a micro environment with proteins such as bone morphogenetic proteins (BPM). Inactive biomaterial particles were not observed. Samples showed 0% and 30% bone tissue respectively. Considering histological differences between this and other research studies, it is necessary to develop further investigation to increase knowledge of processes involved in bone regeneration as well as bone quality, considering the variability that could be seen in each patient.

show abstract

Section: Discussioncontrasting

confidence: 56%

Histological Evaluation of Bone Regeneration Means Freeze Dried Bone Allograft (FDBA) in Post Exodontia Sockets

et al. 2011

View full text Add to dashboard Cite

show abstract

“…[17][18][19][20] BCP has been also used as a carrier for rhBMP-2 in the alveolar ridge defect 21 and spine fusion 22,23 models. Jung et al 24 evaluated rhBMP-2-loaded HA/TCP in the rabbit calvarial onlay model, and concluded that this might be an ideal carrier system for rhBMP-2.…”

Section: Introductionmentioning

confidence: 99%

Recombinant Human Bone Morphogenetic Protein-2 Stimulates the Osteogenic Potential of the Schneiderian Membrane: A Histometric Analysis in Rabbits

Youngik

Lee

Kim

et al. 2013

Tissue Engineering Part A

View full text Add to dashboard Cite

This study evaluated the osteoinductive effect of the recombinant human bone morphogenetic protein-2 (rhBMP-2)-coated biphasic calcium phosphate (BCP) carrier system on the grafted sinus area, including surrounding tissues and the Schneiderian membrane. A total of 18 male rabbits were used in this study; two for in vitro and 16 for in vivo experiments. Schneiderian membranes taken from two animals were cultured with or without rhBMP-2, and quantitative reverse transcriptase-polymerase chain reaction analysis was performed. Both maxillary sinuses in each of the 16 animals were used to compare the in vivo effects of rhBMP-2-coated BCP (experimental group) and BCP alone (control group). In each animal, rhBMP-2-coated BCP was grafted into one of the maxillary sinuses, and the same amount of BCP alone was grafted into the contralateral site in random order. Radiologic and histometric analyses were performed at 2 and 8 weeks after surgery. After 2 days of culturing with or without rhBMP-2, a significant increase in the expression of early osteoblasts (RUNX2, type I collagen, alkaline phosphatase, and osteopontin) could be observed. Different histologic healing patterns were observed in experimental and control sites: newly formed bone lining the reflected sinus membrane without bone formation was observed at the central areas of experimental sites (window=0.06%; center=0%; membrane=20.86% of new bone), whereas evenly distributed new bone formation was observed at the control sites (window=7.27%; center=7.41%; membrane=15.58% of new bone).The augmented volume was well maintained at both the experimental and control sites during the experimental period, but at 2 weeks, the augmented volume was greater at the experimental sites than at the control sites (232.62 and 195.29 mm(3), respectively; p<0.001). These results suggest that good space maintenance in sinus augmentation is achieved with BCP, while the osteoinductive potential of the sinus membrane is activated at the early stage of healing with rhBMP-2.

show abstract

“…2 Biosynthetic substitutes, such as b-tricalcium phosphate (b-TCP), hydroxyapatite (HA), and mixtures of HA/b-TCP (biphasic calcium phosphates; BCP) have been successfully used as a graft material, because of their good biocompatibility and chemical composition, which resembles the composition of the natural bone matrix. [3][4][5][6] An important issue to consider regarding graft material degradation and bone ingrowth is the pore size; large pore sizes ( ‡ 500 mm) promote neovascularization and favor mineralized bone ingrowth, 1000 mm for the BCP scaffolds. Nevertheless, despite these structural optimizations, the lack of osteogenic properties still results in a slower rate of new bone formation.…”

Section: Introductionmentioning

confidence: 99%

Short (15 Minutes) Bone Morphogenetic Protein-2 Treatment Stimulates Osteogenic Differentiation of Human Adipose Stem Cells Seeded on Calcium Phosphate Scaffolds In Vitro

Overman

Farré-Guasch

Helder

et al. 2013

Tissue Engineering Part A

View full text Add to dashboard Cite

A one-step concept for bone regeneration has been postulated in which human adipose stem cells (hASCs) are harvested, triggered to differentiate, seeded on carriers, and implanted in the same operative procedure. Toward this goal it was investigated whether short (minutes) incubation with bone morphogenetic protein-2 (BMP-2) suffices to trigger osteogenic differentiation of hASCs seeded on calcium phosphate carriers. hASCs were treated with or without BMP-2 (10 ng/mL) for 15 min, and seeded on b-tricalcium phosphate granules (b-TCP; sized < 0.7 mm or > 0.7 mm) or biphasic calcium phosphate (BCP; 60%/40% or 20%/80% hydroxyapatite/b-TCP). Attachment was determined after 10-30 min. Proliferation (DNA content) and osteogenic differentiation (alkaline phosphatase activity, gene expression) were analyzed up to 3 weeks of culture. hASC attachment to the different scaffolds was similar, and unaffected by BMP-2. It stimulated gene expression of the osteogenic markers core binding factor alpha 1, collagen-1, osteonectin, and osteocalcin in hASCs seeded on BCP and b-TCP. Downregulation of osteopontin expression by BMP-2 was seen in BCP-seeded cells only. BMP-2 treatment inhibited expression of the adipogenic marker peroxisome proliferator-activated receptor gamma. In conclusion, 15 min BMP-2 preincubation of hASCs seeded on BCP/b-TCP scaffolds had a long-lasting stimulating effect on osteogenic differentiation in vitro. These results strongly support a one-step clinical concept for bone regeneration.

show abstract

Histologic and clinical evaluation for maxillary sinus augmentation using macroporous biphasic calcium phosphate in human

Cited by 45 publications

References 18 publications

Histological Evaluation of Bone Regeneration Means Freeze Dried Bone Allograft (FDBA) in Post Exodontia Sockets

Histological Evaluation of Bone Regeneration Means Freeze Dried Bone Allograft (FDBA) in Post Exodontia Sockets

Recombinant Human Bone Morphogenetic Protein-2 Stimulates the Osteogenic Potential of the Schneiderian Membrane: A Histometric Analysis in Rabbits

Short (15 Minutes) Bone Morphogenetic Protein-2 Treatment Stimulates Osteogenic Differentiation of Human Adipose Stem Cells Seeded on Calcium Phosphate Scaffolds In Vitro

Contact Info

Product

Resources

About