Histologic and Morphometric Analysis of the Choroid, Bruch's Membrane, and Retinal Pigment Epithelium in Postmortem Eyes With Age-Related Macular Degeneration and Histologic Examination of Surgically Excised Choroidal Neovascular Membranes

Spraul, Christoph W.; Lang, Gabriele E.; Grossniklaus, Hans E.; Lang, Gerhard K.

doi:10.1016/s0039-6257(99)00086-7

Cited by 305 publications

(264 citation statements)

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“…Basal deposits are the strongest histopathological marker of aging and age-related disease (Sarks, 1976;Green and Enger, 1993;Spraul et al, 1996;Spraul and Grossniklaus, 1997;Curcio and Millican, 1999). The location and composition of these deposits distinguish aging from AMD.…”

Section: Discussionmentioning

confidence: 99%

“…The location and composition of these deposits distinguish aging from AMD. Basal laminar deposits (BlamD), which form between the RPE cell and basement membrane, are a normal aging change early, but become specific for AMD when they become thick and contain cellular debris, 'long spaced collagen', membranous structures, lipid, and inflammatory proteins (Sarks, 1976;Newsome et al, 1987;Green and Enger, 1993;van der Schaft et al, 1994;Spraul et al, 1996;Spraul and Grossniklaus, 1997;Curcio and Millican, 1999;Anderson and Ozaki, 2001;Johnson et al, 2002;Leu et al, 2002). The most sensitive and specific histopathologic marker of AMD is basal linear deposits (BlinD), which form in the inner collagenous layer of Bruch's membrane.…”

Section: Discussionmentioning

confidence: 99%

“…The composition and location of these deposits distinguishes chronological aging from age-related disease. Basal laminar deposits, which develop between the RPE cell and basement membrane, are specific for age-related macular degeneration (AMD) when they are thick and contain heterogeneous debris such as long spaced collagen (Sarks, 1976;Newsome et al, 1987;Green and Enger, 1993;van der Schaft et al, 1994;Spraul et al, 1996;Spraul and Grossniklaus, 1997;Curcio and Millican, 1999;Anderson et al, 2001;Johnson et al, 2002;Leu et al, 2002). Basal linear deposits occur within the inner collagenous layer, and are the most specific basal deposit for AMD (Sarks, 1976;Newsome et al, 1987;Green and Enger, 1993;van der Schaft et al, 1994;Spraul et al, 1996;Spraul and Grossniklaus, 1997;Curcio and Millican, 1999;Anderson et al, 2001;Johnson et al, 2002;Leu et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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The expression of advanced glycation endproduct receptors in rpe cells associated with basal deposits in human maculas

Yamada

Ishibashi

et al. 2006

Experimental Eye Research

100

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Basal deposits within Bruch's membrane are associated with aging and age-related macular degeneration (AMD) although the factors causing their formation are incompletely understood. Advanced glycation endproducts (AGEs) accumulate in Bruch's membrane including basal deposits and drusen with aging. One mechanism by which AGEs alter a cell's phenotype is via AGE receptors. The purpose of this study was to immunolocalize and quantify the expression of AGE receptors by RPE cells associated with basal deposits or normal Bruch's membrane that were microdissected from human maculas. Postmortem eyes from 14 aged control donors and five donors with non-neovascular AMD were cryopreserved. RPE cells associated with normal Bruch's membrane or basal deposits were laser capture microdissected. The RNA was extracted and used for RT-qPCR to quantify the expression of RAGE, AGE R1, AGE R2, and AGE R3. Streptavidin alkaline phosphatase immunohistochemistry for these receptors was also performed and sections were bleached from 14 normal and nine AMD donors. RT-qPCR showed significant upregulation of RAGE, AGE R1, and AGE R3 in RPE cells overlying basal deposits compared to cells attached to morphologically normal Bruch's membrane. Immunohistochemical analysis for RAGE, AGER1, R2, and R3 showed diffuse, light staining of RPE cells and strong choriocapillaris staining in areas of normal Bruch's membrane. In areas of basal deposits, the RPE had more intense staining for RAGE and AGER1 compared to regions of normal Bruch's membrane. These results suggest that AGE receptors could influence the formation of basal deposits during aging and AMD.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The expression of advanced glycation endproduct receptors in rpe cells associated with basal deposits in human maculas

Yamada

Ishibashi

et al. 2006

Experimental Eye Research

100

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“…Distended and tortuous choroidal veins 1,5 have also been reported. The origin of the latter has been linked to the increased scleral rigidity in patients with ARM as described by Friedman et al 23 The same authors further proposed that the increased scleral rigidity might increase resistivity to choroidal blood inflow and outflow.…”

Section: (1) Study Of Retrobulbar Circulation In Stages Of Armmentioning

confidence: 97%

“…Morphologic alterations in the retinal pigment epithelium (RPE)-Bruch's membrane complex and the choroid have been described. [1][2][3][4] Although the etiology of ARM is not clearly understood, vascular irregularities are present in ARM and a blood circulatory dysfunction has been proposed in the pathogenesis of this disease. [5][6][7][8][9] Because a number of reports [10][11][12][13] have provided qualitative data suggesting abnormalities of the choroidal circulation in ARM, quantitative assessment of the choroidal circulation has been thought to be important for investigating the etiologic role of ocular perfusion in the development of ARM.…”

Section: Introductionmentioning

confidence: 99%

Retrobulbar circulation in patients with age-related maculopathy

Dimitrova

Tamaki

Kato

2002

Eye

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circulation in patients with age-related maculopathy AbstractPurpose To investigate retrobulbar circulation in stages of age-related maculopathy (ARM) and in normal fellow eyes of patients with unilateral exudative ARM. Methods Color Doppler Imaging was used to measure circulatory parameters (peak-systolic velocity-PSV, end-diastolic velocity-EDV, pulsatility index-PI and resistivity index-RI) in the central retinal, posterior ciliary and ophthalmic arteries of 44 patients with age-related maculopathy (ARM) and 32 control subjects. Patients with ARM consisted of 11 with early ARM and 33 with late ARM. Twenty-one patients from the exudative ARM group were also included in a study of unilateral exudative ARM circulation. Results In the study of patients with ARM divided into stages, the PI and RI in the posterior ciliary artery of patients with late ARM were significantly higher than that of the control group (P ‫؍‬ 0.0064; P ‫؍‬ 0.0124). In the early ARM group, circulatory parameters did not differ significantly from those of the control group.In the study of unilateral exudative ARM, the affected eye showed significantly higher RI, as compared to the control group (P ‫؍‬ 0.0157), and the fellow eye had significantly decreased EDV as compared to the control group (P ‫؍‬ 0.0164). There was no significant difference in circulatory parameters between the affected and normal fellow eyes of patients with unilateral exudative ARM. Conclusion PI and RI in patients with late ARM and EDV in fellow eyes of patients with unilateral exudative ARM showed significant changes in the posterior ciliary artery compared to normal controls. Altered circulation in the posterior ciliary artery may be involved in the pathogenesis of ARM.

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A Fluorescein and Indocyanine Green Angiographic Study of Choriocapillaris in Age-related Macular Disease

Pauleikhoff¹

1999

Arch Ophthalmol

107

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To examine the phenomenon of a prolonged choroidal filling phase (PCFP) as seen on fluorescein and indocyanine green (ICG) angiography in patients with early age-related macular disease (AMD). Methods: One hundred eyes of consecutive patients with early AMD were studied. Patchy and slow choroidal filling in early fluorescein and distinct areas of reduced choroidal fluorescence in ICG angiography were interpreted as PCFP. In addition, associated drusen characteristics and the AMD status of the fellow eye were recorded. Results: A PCFP was observed in 26% of eyes using fluorescein and 32% of eyes using ICG angiography, with good concordance between findings using both techniques (= 0.9). A PCFP was associated with confluent drusen

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Histologic and Morphometric Analysis of the Choroid, Bruch's Membrane, and Retinal Pigment Epithelium in Postmortem Eyes With Age-Related Macular Degeneration and Histologic Examination of Surgically Excised Choroidal Neovascular Membranes

Cited by 305 publications

References 69 publications

The expression of advanced glycation endproduct receptors in rpe cells associated with basal deposits in human maculas

The expression of advanced glycation endproduct receptors in rpe cells associated with basal deposits in human maculas

Retrobulbar circulation in patients with age-related maculopathy

A Fluorescein and Indocyanine Green Angiographic Study of Choriocapillaris in Age-related Macular Disease

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