Histologic characteristics of human intestinal spirochetosis in operatively resected specimens

Ogata, Sho; Shimizu, Ken; Tominaga, Susumu; Nakanishi, Kuniaki

doi:10.1007/s00428-020-02785-w

Cited by 9 publications

(13 citation statements)

References 15 publications

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“…The fringe formation on the mucosal surface) may be too subtle or focal for it to be easily recognized even by experienced pathologists. Moreover, the diagnostic fringes are not always found everywhere in the large intestine of HIS cases [12] and we noted that stromal or intraepithelial eosinophil in ltration (sEo or iEo, respectively) was not in uenced by the degree of fringe distribution. In the present study, intraepithelial eosinophil counts were related not only to their stromal counts, but also to both the intraepithelial and stromal neutrophil counts.…”

Section: Discussionmentioning

confidence: 64%

“…These ndings suggest the presence of HIS-related chronic mucosal in ammation, although the presence of LFs did not in uence eosinophils, neutrophils, or mast cells counts or the spirochetal infection parameters that we examined. In our previous immunohistochemical investigation using surgically removed specimens with HIS, possible spirochetal fragments were detected within LFs [12]. On that basis, LFs might be another immunologic host reaction to a spirochetal burden.…”

Section: Discussionmentioning

confidence: 81%

“…The present study has some limitations regarding its materials: (1) we analyzed a small number of HIS cases that were archived and that were detected at only a single hospital (although it is a medical center in a location neighboring Tokyo, the capital of Japan, and specimens were also collected from other hospitals in the same prefecture) in only three calendar years, (2) potential inaccuracy may have resulted from IHC cross-reaction with the antibody used in the present study (antibodies speci c for Brachyspira species not being commercially available), and (3) the in ammation parameters were limited to a few cell types, and excluded lymphocytes, plasma cells, and macrophages (these cells, especially macrophages, might contribute to the mucosal immunity to tissue-invading spirochetes [12], although examining them was beyond the scope of present study).…”

Section: Discussionmentioning

confidence: 99%

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Mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosis

Ogata

Shimizu

Tominaga

et al. 2021

Preprint

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Background: Human intestinal spirochetosis (HIS) is an infectious disease of large intestines caused by Brachyspira species, and most HIS cases are asymptomatic or exhibit mild intestinal symptoms. The host reaction to HIS remains unclear, and we examined HIS-related mucosal inflammatory features histologically. Method: From the archival HIS cases in a single medical center, 24 endoscopically taken specimens from 14 HIS cases (M:F = 10:4; 28-73 yrs) were selected as not containing polypoid or neoplastic lesions. Stromal neutrophils, eosinophils, and mast cells, and intraepithelial neutrophils and eosinophils, (sNeu, sEo, sMast, iNeu, and iEo, respectively) were counted, and the presence or absence of lymphoid follicles/aggregates (LFs) was also examined. Association of the above inflammation parameters and spirochetal infection parameters (such as degrees of characteristic fringe distribution, of spirochetal cryptal invasion, and of spirochetal intraepithelial invasion) were also analysed. Results: iNeu was observed in 29.2%, iEo in 58.3%, and LFs in 50.0% of the specimens. Maximal counts of sNeu, sEo, sMast, iNeu, and iEo averaged 8.4, 21.5, 6.0, 0.5 and 1.5, respectively. Strong correlation between the maximum counts of iNeu and iEo (p < 0.001, r = 0.81), and correlations between those of iEo and sNeu (p = 0.0012, r = 0.62) and between those of iEo and sEo (p = 0.026, r = 0.45) were observed. iNeu was influenced by fringe formation (p < 0.05) and spirochetal crypt involvement (p < 0.05). Conclusions: HIS was accompanied by inflammatory reactions, and among these, mucosal eosinophilic infiltration may be a central indicator and host reaction of HIS.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosis

Ogata

Shimizu

Tominaga

et al. 2021

Preprint

Self Cite

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show abstract

“…the fringe formation on the mucosal surface) may be too subtle or focal for it to be easily recognized, even by experienced pathologists. Moreover, the diagnostic fringes are not always found everywhere in the large intestine of HIS cases [ 13 ] and we noted that stromal or intraepithelial eosinophil infiltration (sEo or iEo, respectively) was not influenced by the degree of fringe distribution. In the present study, intraepithelial eosinophil counts were related not only to their stromal counts, but also to both the intraepithelial and stromal neutrophil counts.…”

Section: Discussionmentioning

confidence: 82%

“…These findings suggest the presence of HIS-related chronic mucosal inflammation, although the presence of LFs did not influence eosinophils, neutrophils, or mast cells counts or the spirochetal infection parameters that we examined. In our previous immunohistochemical investigation using surgically removed specimens with HIS, possible spirochetal fragments were detected within LFs [ 13 ]. On that basis, LFs might be another immunologic host reaction to a spirochetal burden.…”

Section: Discussionmentioning

confidence: 99%

Mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosis

et al. 2021

Self Cite

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Background Human intestinal spirochetosis (HIS) is an infectious disease of large intestines caused by Brachyspira species, and most HIS cases are asymptomatic or exhibit mild intestinal symptoms. The host reaction to HIS remains unclear, and we examined HIS-related mucosal inflammatory features histologically. Methods From the archival HIS cases in a single medical center, 24 endoscopically taken specimens from 14 HIS cases (male:female = 10:4; 28–73 yrs) were selected as not containing polypoid or neoplastic lesions. Stromal neutrophils, eosinophils, and mast cells, and intraepithelial neutrophils and eosinophils, (sNeu, sEo, sMast, iNeu, and iEo, respectively) were counted, and the presence or absence of lymphoid follicles/aggregates (LFs) was also examined. Association of the above inflammation parameters and spirochetal infection parameters (such as degrees of characteristic fringe distribution, of spirochetal cryptal invasion, and of spirochetal intraepithelial invasion) were also analysed. Results iNeu was observed in 29.2%, iEo in 58.3%, and LFs in 50.0% of the specimens. Maximal counts of sNeu, sEo, sMast, iNeu, and iEo averaged 8.4, 21.5, 6.0, 0.5 and 1.5, respectively. Strong correlation between the maximum counts of iNeu and iEo (p < 0.001, r = 0.81), and correlations between those of iEo and sNeu (p = 0.0012, r = 0.62) and between those of iEo and sEo (p = 0.026, r = 0.45) were observed. iNeu was influenced by fringe formation (p < 0.05) and spirochetal crypt involvement (p < 0.05). Conclusions HIS was accompanied by inflammatory reactions, and among these, mucosal eosinophilic infiltration may be a central indicator and host reaction of HIS.

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Pathologie infectieuse du tube digestif : diagnostics à ne pas manquer

Guenzi

Chen

Sannier

et al. 2023

Annales de Pathologie

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Histologic characteristics of human intestinal spirochetosis in operatively resected specimens

Cited by 9 publications

References 15 publications

Mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosis

Mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosis

Mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosis

Pathologie infectieuse du tube digestif : diagnostics à ne pas manquer

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