Histologic chorioamnionitis does not modulate the oxidative stress and antioxidant status in pregnancies complicated by spontaneous preterm delivery

Martín, Luiz A. B. San; Moço, Natália Prearo; Lima, Moisés Diôgo de; Polettini, Jossimara; Miot, Hélio Amante; Corrêa, Camila Renata; Menon, Ramkumar; Silva, Márcia Guimarães da

doi:10.1186/s12884-017-1549-4

Cited by 15 publications

(11 citation statements)

References 56 publications

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“…The competitive 3-nitrotyrosine (3-NT) ELISA Kit was used to determine protein peroxidation in plasma (OxiSelect™ Nitrotyrosine ELISA Kit, Cell Biolabs Inc., San Diego, USA). 3-NT is a product of posttranslational modification of protein tyrosine caused by peroxynitrite, and this modification can result in changes in protein structure, function, and catalytic activity [28]. The absorbance was read at 450 nm against 620 nm as a reference with an ELISA reader iMark™ (Bio-Rad USA).…”

Section: Methodsmentioning

confidence: 99%

“…The measurement of protein carbonyl content is considered a reliable marker of oxidative protein damage due to its long-lasting stability [28]. Protein carbonyls were measured as a parameter of oxidative stress in plasma after derivatization with 2,4-dinitrophenylhydrazine (DNPH) with the Carbonyl Protein ELISA Kit (Immundiagnostik AG, Bensheim, Germany) according to the instructions supplied by the manufacturer.…”

Section: Methodsmentioning

confidence: 99%

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Oxidative Stress in Women Treated with Atosiban for Impending Preterm Birth

Grzesiak

Gaj

Kocyłowski

et al. 2018

Oxidative Medicine and Cellular Longevity

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Preterm birth is defined as delivery before 37 completed weeks of pregnancy, and it is the leading cause of neonatal morbidity and mortality. Oxidative stress is recognized as an important factor in the pathogenesis of premature labor. We conducted this analysis to investigate the safety of administration of the tocolytic drug Atosiban—a reversible, competitive antagonist of the oxytocin receptor in the treatment of preterm birth and its impact on the level of oxidative stress in pregnant women after 48 hours of tocolytic treatment. This prospective study was conducted between March 2016 and August 2017 at the Obstetric Clinic of the Polish Mother's Memorial Hospital Research Institute. Total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) values as well as 3-nitrotyrosine, carbonyl, and thiol group levels were measured using an ELISA test in serum and plasma of 56 pregnant women before and after 48 hours of continuous administration of Atosiban. We found that TAS levels decreased almost twice after the 48-hour drug administration (0.936 ± 0.360 mmol/L vs. 0.582 ± 0.305 mmol/L, P < 0.001) while TOS increased from 18.217 ± 16.093 μmol/L to 30.442 ± 30.578 μmol/L (P < 0.001). We also found a significant increase in OSI index—almost a threefold increase from 0.022 ± 0.022 to 0.075 ± 0.085, P < 0.001. In addition, statistically significant differences in the level of carbonyl groups were found. It increased from 65.358 ± 31.332 μmol/L to 97.982 ± 38.047 μmol/L (P < 0.001), which indicates increased oxidation of plasma proteins. Furthermore, patients who gave birth prematurely had higher levels of TOS after a 48-hour drug administration than the second group with labor after 37 weeks of pregnancy (42.803 ± 34.683 μmol/L vs. 25.792 ± 27.821 μmol/L, P < 0.031). The obtained results clearly indicate that pregnant women during tocolytic treatment with Atosiban are in a state of increased oxidative stress and occurrence of preterm birth can be associated with this phenomenon. This trial is registered with NCT03570294.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Oxidative Stress in Women Treated with Atosiban for Impending Preterm Birth

Grzesiak

Gaj

Kocyłowski

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…Therefore, as is well-known, inflammation is a key point in parturition, even in the absence of intrauterine infection, and, in such cases, inflammation may come from the senescence of fetal membranes ( Behnia et al, 2016 ; Martin et al, 2017 ). Behnia et al (2015) found higher concentrations of pro-inflammatory SASP markers (granulocyte macrophage colony-stimulating factor and interleukin-6 and -8) in the amniotic fluid of women in labor at term than in women not in labor.…”

Section: Fetal Membranes Telomere-senescence In Parturition and Advermentioning

confidence: 99%

Telomere-Related Disorders in Fetal Membranes Associated With Birth and Adverse Pregnancy Outcomes

Polettini

Silva

2020

Front. Physiol.

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Telomere disorders have been associated with aging-related diseases, including diabetes, vascular, and neurodegenerative diseases. The main consequence of altered telomere is the induction of the state of irreversible cell cycle arrest. Though several mechanisms responsible for the activation of senescence have been identified, it is still unclear how a cell is indeed induced to become irreversibly arrested. Most tissues in the body will experience senescence throughout its lifespan, but intrinsic and extrinsic stressors, such as chemicals, pollution, oxidative stress (OS), and inflammation accelerate the process. Pregnancy is a state of OS, as the higher metabolic demand of the growing fetus results in increased reactive oxygen species production. As a temporary organ in the mother, senescence in fetal membranes and placenta is expected and linked to term parturition (>37 weeks of gestation). However, a persistent, overwhelming, or premature OS affects placental antioxidant capacity, with consequent accumulation of OS causing damage to lipids, proteins, and DNA in the placental tissues. Therefore, senescence and its main inducer, telomere length (TL) reduction, have been associated with pregnancy complications, including stillbirth, preeclampsia, intrauterine growth restriction, and prematurity. Fetal membranes have a notable role in preterm births, which continue to be a major health issue associated with increased risk of neo and perinatal adverse outcomes and/or predisposition to disease in later life; however, the ability to mediate a delay in parturition during such cases is limited, because the pathophysiology of preterm births and physiological mechanisms of term births are not yet fully elucidated. Here, we review the current knowledge regarding the regulation of telomere-related senescence mechanisms in fetal membranes, highlighting the role of inflammation, methylation, and telomerase activity. Moreover, we present the evidences of TL reduction and senescence in gestational tissues by the time of term parturition. In conclusion, we verified that telomere regulation in fetal membranes requires a more complete understanding, in order to support the development of successful effective interventions of the molecular mechanisms that triggers parturition, including telomere signals, which may vary throughout placental tissues.

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“…If chorioamnionitis is found in more than 20% of placenta specimens, the clinical significance increases. It may lead to preterm delivery, fetal and neonatal infection, respiratory distress, intrauterine hypoxia, and low APGAR score (1,2,21). In chorioamnionitis, the chronic villitis plays significant role in fetal infection; more than 30% of villous degeneration leads to perinatal death (1,2).…”

Section: Discussionmentioning

confidence: 99%