Histologic comparison of polycystic ovary syndrome induced by estradiol valerate and letrozole

Lim, Sung Chul; Jeong, Moon Jin; Kim, Se Eun; Kim, Seung Hyun; Kim, Sam Cheol; Seo, Seung Yong; Kim, Tak; Kang, Seong Soo; Bae, Chun Sik

doi:10.5468/kjog.2011.54.6.294

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…However, neither L nor EV has generated oxidative stress. In addition, the ultrasound assessment of ovaries revealed increased dimensions of the ovaries in the L group as compared with the EV group, result which is comparable with other histopathological findings (Lim et al 2011). Also, transonic images were visualized after EV administration, in agreement with our previous ultrasonographic and histopathological results (Dȃneasȃ et al 2014).…”

Section: Discussionsupporting

confidence: 89%

Letrozole vs estradiol valerate induced PCOS in rats: glycemic, oxidative and inflammatory status assessment

et al. 2016

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The objective of our study was to investigate glycemic, oxidative/antioxidative and inflammatory status in letrozole and estradiol valerate induced polycystic ovarian syndrome (PCOS) models. Sixty adult female Wistar rats were divided into four groups: L (0.2 mg letrozole/0.5 ml carboxymethyl cellulose (CMC), daily for 30 days), the control group C L , EV (one i.m. injection of 5 mg EV/0.5 ml sesame oil) and its corresponding control group C EV . After 30 days, ovarian morphology was assessed through ultrasound, serum free testosterone was determined, and an oral glucose tolerance test was performed. Blood, muscle, liver and periovarian adipose tissue (POAT) were collected for oxidative/antioxidative and inflammatory status evaluation. Free testosterone was increased only in the L group, while fasting glycemia was higher in the EV group. Both L and EV led to a significantly decreased level of muscle malondialehyde (MDA) and liver glutathione peroxidase (GPx) activity, while in POAT, MDA level diminished and GPx activity increased. The only difference between the two protocols was in muscle, where after L administration, GPx activity was significantly lower. Implementation of both protocols resulted in an increased expression of pNFKB in muscle, liver and POAT. The expression of monocyte chemoattractant protein 1 (MCP1) increased in liver and POATafter L administration, while in the EV group, MCP1 and STAT3 decreased in POAT. Our study shows that both protocols are characterized by an inflammatory environment in the usually insulin resistant tissues of human PCOS, without generating oxidative stress. In addition, EV has mild metabolic effects and unexpected interference with MCP1 expression in POAT, which require further investigation.

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Section: Discussionsupporting

confidence: 89%

Letrozole vs estradiol valerate induced PCOS in rats: glycemic, oxidative and inflammatory status assessment

et al. 2016

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“…The thin granulosa layer, thickened theca layer, and degenerative nuclear structures were determined for cyst volume analysis (Wu et al, 2014;Wang et al, 2012;Lim et al, 2011). No statistically signi cant difference was observed among the groups (p > 0.05; One-Way ANOVA) (Fig.…”

Section: Volume Resultsmentioning

confidence: 99%

An investigation of the potential effects of amitriptyline on polycystic ovary syndrome induced by estradiol valerate

Alkan

Kaplan

2023

Histochem Cell Biol

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Polycystic ovarian syndrome (PCOS) is frequently observed in adolescent women and usually progresses with depression. The aim of this study was to examine the effects of amitriptyline (AMI), a drug used in the treatment of depression, in individuals with PCOS. Forty 12-week-old female Wistar albino rats were randomly divided into ve groups; control, sham, PCOS, AMI, PCOS+AMI. In order to induce the syndrome in the PCOS groups, a single dose of 4mg/kg estradiol valerate was administered by intraperitoneal injection, 10mg/kg AMI was administered by intraperitoneal injection for 30days in the AMI groups. After 30days, all the animals were sacri ced and blood, ovary, brain tissues were collected, subjected to routine tissue processing. Stereological, histopathological analyses were performed on the ovarian sections, while LH, FSH, CAT, and SOD levels were investigated in blood samples. The volume of the corpus luteum and preantral follicles increased in the PCOS group, while a decrease was determined in the number of antral follicles using stereological methods. Biochemical analysis revealed that FSH levels increased and CAT enzyme levels decreased in the PCOS group. Signi cant morphological changes were observed in ovaries from the PCOS group. The volume of the corpus luteum in the PCOS+AMI group decreased compared to the PCOS group. Serum FSH levels decreased in the PCOS+AMI group, while CAT enzyme levels increased compared to the PCOS group. Degenerative areas were also seen in the PCOS+AMI group ovaries. AMI administration was unable to su ciently ameliorate the morphological and biochemical changes caused in the ovarian tissues by PCOS.

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Synergistic Adenosine Triphosphate/Chitosan Bio-coatings on Polyurethane Foam for Simultaneously Improved Flame Retardancy and Smoke Suppression

Song

Park

Jeong

et al. 2023

ACS Appl. Polym. Mater.

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Considering that fire safety is a persistent problem for most polymeric materials, including polyurethane (PU) foam, the demand for flame retardants (FRs) is growing. However, the use of conventional FRs containing halogenated and brominated chemicals has been continuously regulated owing to their toxicity. Here, we demonstrate the layer-by-layer (LbL) coating of negatively charged adenosine triphosphate (ATP) and positively charged chitosan (CS) as the synergistic FR on PU foam, a model flammable polymer material. The FR performance of the PU coated with LbL-assembled ATP and CS (ATP/CS-PU) was tested, and the results suggested that the ATP/CS layers were finely deposited on the surface of the PU without damaging the structure. Only five bilayers (5BL) were sufficient to impart excellent fire retardancy, which exhibited a limiting oxygen index value of 35% and the HF-1 rating in the UL94 foamed material horizontal burning test. In addition, ATP/CS(5BL)-PU showed a significant reduction in the peak heat release rate of 42.0% and in the total smoke release of 30.6% compared to that of bare PU (b-PU). Furthermore, the ATP/CS coatings did not deteriorate the mechanical properties of b-PU. Finally, combined thermogravimetry-Fourier transform infrared spectroscopy (TG-FTIR) showed that ATP/CS(5BL)-PU was safe because it suppresses hazardous gases, which is the main problem with conventional FRs.

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Histologic comparison of polycystic ovary syndrome induced by estradiol valerate and letrozole

Cited by 4 publications

References 20 publications

Letrozole vs estradiol valerate induced PCOS in rats: glycemic, oxidative and inflammatory status assessment

Letrozole vs estradiol valerate induced PCOS in rats: glycemic, oxidative and inflammatory status assessment

An investigation of the potential effects of amitriptyline on polycystic ovary syndrome induced by estradiol valerate

Synergistic Adenosine Triphosphate/Chitosan Bio-coatings on Polyurethane Foam for Simultaneously Improved Flame Retardancy and Smoke Suppression

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