Histologic evaluation of artificial floors under MTA and Nano-filled resin-modified glass ionomer used to repair furcation perforation in dogs

Abstract

Objective: This study aimed to compare the tissue reaction of two repair materials for furcation perforations, nano-filled resin modified glass ionomer (Nano-FRMGI) and mineral trioxide aggregate (MTA), used with or without an artificial floor. Methods: A total of 96 teeth in 6 dogs were used for this study. After access cavities, root canals were prepared and obturated with gutta percha using cold lateral condensation technique. Perforations were then created on the floors of the pulp chambers. The perforations divided into four groups n=24/group that were sealed with MTA alone, MTA with calcium sulphate artificial floor (CSAF), FRMGI alone and Nano-FRMGI with CSAF. All access cavities were filled with composite resin. Two dogs were sacrificed at 1, 3, and 6 month. The experimental tooth along with the surrounding alveolar bone were cut in block sections and histologically evaluated for tissue response. Data were analyzed by Chi-square (P≤0.05). Results: MTA and MTA with CSAF showed more bone and cementum apposition when compared to Nano-FRMGI at 6-month interval. MTA and MTA with CSAF showed less bone resorption, epithelium proliferation and inflammation compared to Nano-FRMGI at 6-month interval. Conclusion: MTA with CSAF or MTA-alone show better outcomes in the repair of pulp chamber floor perforation.

Effect of epigallocatechin-3-gallate (EGCG) - based paste as intracanal dressing, in matrix metalloproteinases 2 and 9 in dog’s periapical lesions

“…In the present study we use beagle dogs as model for histopathological study of apical periodontitis due its anatomical and physiopathological similarities with humans. Experimental induction of apical periodontits in dogs teeth has being performed by histopathological studies for evaluation of new endodontic materials and modalities of treatment under the same operatory conditions applied in humans and with healing responses obtained in shorter periods than humans [30].…”

Does MTA provide a more favourable histological response than other materials in the repair of furcal perforations? A systematic review

“…Their protocols for outcome assessment differed in, for example, observation time points and outcome assessment methods. Many studies assessed these characteristics by means of scores (Aladimi et al, 2020; Al‐Daafas & Al‐Nazhan, 2007; Bakhtiar et al, 2017; Cardoso et al, 2018b; Noetzel et al, 2006; Samiee et al, 2010; Silva et al, 2019; de Sousa Reis et al, 2019) or cell counting (da Fonseca et al, 2019; Hassanien et al, 2015; Neto et al, 2010, 2012; da Silva et al, 2011; Silva et al, 2017; Tawfik et al, 2016). Even those studies that analysed the outcomes by means of scores evaluated different characteristics in each score.…”

“…Despite the biological characteristics of repair materials, their extrusion to the surrounding tissues may compromise the success of furcal perforation repair (Al‐Daafas & Al‐Nazhan, 2007; Mente et al, 2010). Several studies evaluated the use of calcium sulphate as an artificial floor/barrier on the perforated floor to control placement and avoid material extrusion (Aladimi et al, 2020; Al‐Daafas & Al‐Nazhan, 2007; Bakhtiar et al, 2017; Neto et al, 2010, 2012). Most of these studies found that the addition of this barrier did not improve treatment outcomes.…”

“…Materials with different compositions affect the response of teeth and their surrounding tissues (Aladimi et al, 2020). Although randomized clinical trials are the reference standard for the comparison of materials and methods, few observational studies (de Chevigny et al, 2008; Farzaneh et al, 2004; Gorni et al, 2016; Gorni & Gagliani, 2004; Mente et al, 2010; Ng et al, 2011) and no randomized clinical trials have investigated root canal perforations, because of ethical issues and difficulties in standardizing procedural accidents.…”