Histologic Evaluation of Guided Tissue Regeneration Using 4 Barrier Membranes: A Comparative Furcation Study in Dogs

Lekovic, Vojislav; Klokkevold, Perry R.; Kenney, E. Barrie; Dimitrijelic, Bozidar; Nedić, Milica; Weinlaender, Michael

doi:10.1902/jop.1998.69.1.54

Cited by 22 publications

(18 citation statements)

References 20 publications

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“…This result is consistent with that found by Deliberador et al (2006), in which the control treatment group made with calcium sulfate membranes showed high diffuse inflammatory infiltration. Lekovic et al (1998), in a study of four membranes, one of polycaprolactone, results showed a good formation of cementum and alveolar bone, however, the membrane comprising polycaprolactone showed high inflammatory response, as seen in the present study. Figure 6.…”

Section: Resultssupporting

confidence: 65%

Treatment of periodontal disease with guided tissue regeneration technique using a hydroxyapatite and polycaprolactone membrane

Martins

Valente

Reis

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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The aim of this study was to evaluate the use of a malleable membrane composed of hydroxyapatite (60%) and polycaprolactone (40%) as treatment of periodontal disease experimentally induced in dogs. A bone defect of standardized dimensions was created between the roots of the third and fourth premolar of 12 dogs for periodontal disease induction. Six dogs had the defect covered by the membrane and six dogs received only standard treatment for periodontal disease, also applied to dogs in the treated group. The animals were clinically monitored during the experiment. Radiographs were taken after surgery and at 60 days after treatment initiation. Clinical attachment level was also assessed in those moments. On the 60th day, dental sample of all animals, containing tooth, defect and periodontal tissues, were harvested, fixed in formalin and analyzed by microtomography and histology. During the experimental period, the animals showed no pain and purulent discharge, however, there was dehiscence in 50% of animals and membrane exposure in five out of six animals in the treated group. Clinical attachment level showed no difference between groups. Radiographs showed radiopacity equal to the alveolar bone in both groups. The microtomography revealed that the control group had higher bone volume in the defect compared to the treated group; however, the furcation was not filled by new alveolar bone in any animal. Histological analysis revealed that junctional epithelium invasion was lighter in the control group. New bone was only observed in the apical edge of the defect in both groups. Although the composite is biocompatible and able to keep the space of the defect, it did not promote periodontal tissue regeneration within 60 days of observation.

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Section: Resultssupporting

confidence: 65%

Treatment of periodontal disease with guided tissue regeneration technique using a hydroxyapatite and polycaprolactone membrane

Martins

Valente

Reis

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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“…In contrast, the pathogenesis of natural periodontal lesions in canines closely resembles the human situation and represents the animal disease model closest to humans in terms of healing and pathogen-associated microbiota (Syed et al, 1981;Giannobile et al, 1994;Madianos et al, 1994). Natural periodontal lesions have been used to test the effects of biologics such as growth factors and barrier membranes on periodontal regeneration (Lynch et al, 1991;Giannobile et al, 1994;Lekovic et al, 1998). Histomorphometric endpoints include the area or height of newly formed bone, cementum, complete new attachment formation (new bone cementum and PDL; CNAA), osseous defect fill, root resorption, and ankylosis.…”

Section: Canine As Large Animal Defect Model Natural Periodontal Lesionsmentioning

confidence: 99%

Pre-clinical Models for Oral and Periodontal Reconstructive Therapies

et al. 2009

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The development of new medical formulations (NMF) for reconstructive therapies has considerably improved the available treatment options for individuals requiring periodontal repair or oral implant rehabilitation. Progress in tissue engineering and regenerative medicine modalities strongly depends on validated pre-clinical research. Preclinical testing has contributed to the recent approval of NMF such as GEM 21S ® and INFUSE ® bone grafts for periodontal and oral regenerative therapies. However, the selection of a suitable preclinical model for evaluation of the safety and efficacy of a NMF remains a challenge. This review is designed to serve as a primer to choose the appropriate pre-clinical models for the evaluation of NMF in situations requiring periodontal or oral reconstruction. Here, we summarize commonly used pre-clinical models and provide examples of screening and functional studies of NMF that can be translated into clinical use.

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“…Furthermore, periodontal diseases can be easily induced in dogs. [12][13][14][15][16] Although subgingival plaque formation in dogs may not develop identically to that in humans, dogs may still serve as a conventional model for investigation.…”

mentioning

confidence: 99%

Autologous Dental Pulp Stem Cells in Regeneration of Defect Created in Canine Periodontal Tissue

Khorsand

Eslaminejad

Arabsolghar

et al. 2013

Journal of Oral Implantology

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This study aimed to investigate effects of dental pulp stem cells (DPSCs) on regeneration of a defect experimentally created in the periodontium of a canine model. Surgically created mesial 3-walled periodontal defects with ligature-induced periodontitis were produced bilaterally in the first lower premolar teeth of 10 mongrel dogs. Simultaneously, DPSCs were derived from the maxillary premolar teeth of the same dogs. Four weeks after creation of the periodontitis model, autologous passaged-3 DPSCs combined with Bio-Oss were implanted on one side as the test group. On the other side, only Bio-Oss was implanted as a control. Eight weeks after surgery, regeneration of the periodontal defects was evaluated histologically and histomorphometrically in terms of bone, periodontal ligament (PDL), and cement formation. Histologically, in all test specimens (10 defects), regeneration of cementum, bone, and PDL was observed. In the control groups, although we observed the regeneration of bone in all defects, the formation of cementum was seen in 9 defects and PDL was seen in 8 defects. Histomorphometric analyses showed that the amount of regenerated cementum and PDL in the test groups (3.83 ± 1.32 mm and 3.30 ± 1.12 mm, respectively) was significantly higher than that of the control groups (2.42 ± 1.40 mm and 1.77 ± 1.27 mm, respectively; P< .05). A biocomplex consisting of DPSCs and Bio-Oss would be promising in regeneration of periodontal tissues.

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Histologic Evaluation of Guided Tissue Regeneration Using 4 Barrier Membranes: A Comparative Furcation Study in Dogs

Cited by 22 publications

References 20 publications

Treatment of periodontal disease with guided tissue regeneration technique using a hydroxyapatite and polycaprolactone membrane

Treatment of periodontal disease with guided tissue regeneration technique using a hydroxyapatite and polycaprolactone membrane

Pre-clinical Models for Oral and Periodontal Reconstructive Therapies

Autologous Dental Pulp Stem Cells in Regeneration of Defect Created in Canine Periodontal Tissue

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