Histologic evaluation of myoepithelial participation in salivary gland tumors

Nikai, Hiromasa; El-Bardaie, Adel Mohamed; Takata, Takashi; Ogawa, Ikuko; Ijuhin, Naokuni

doi:10.1016/s0300-9785(86)80066-7

Cited by 30 publications

(14 citation statements)

References 15 publications

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“…This observation supports the notion that MECs play a part in the formation of myxoid structure [16,18,19,54] by their active production of extracellular matrix [52,53]. However, the present immunohistochemical study did not shed any light on the controversy about the MEC origin of the chondroid and plasmacytoid cells [19,23,25,37,39,41]. In adenoid cystic carcinomas, on the other hand, the luminal layer increased more frequently than the peripheral layer.…”

Section: Discussionsupporting

confidence: 83%

Keratin 14 immunoreactive cells in pleomorphic adenomas and adenoid cystic carcinomas of salivary glands

et al. 2000

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Our recent study of developing myoepithelial cells (MECs) in rat salivary glands demonstrated that developing MECs begin to express alpha-smooth muscle actin (alphaSMA) first and, thereafter, keratin 14. Therefore, it is unlikely that duct basal cells expressing keratin 14 alone are immature or undifferentiated MECs. In this study we carried out immunohistochemistry of pleomorphic adenomas and adenoid cystic carcinomas including normal salivary glands using monoclonal antibodies to keratin 14, smooth muscle proteins and keratin 19. The smooth muscle proteins examined included alphaSMA, h-caldesmon and h1-calponin; h1-calponin was observed in keratinocytes and nerve fibers, indicating that the protein is not specific to smooth muscle, whereas alphaSMA and h-caldesmon turned out to be highly specific markers for smooth muscle cells in normal tissues. In normal glands, MECs were positive for both keratin 14 and smooth muscle proteins (alphaSMA and h-caldesmon). Non-MEC cells were essentially devoid of smooth muscle proteins. Non-MEC duct basal cells expressed keratin 14 with or without keratin 19, and luminal cells keratin 19 with or without keratin 14. This suggests that the keratin 14-positive, smooth muscle proteins-negative duct basal cells are luminal cell progenitors. Luminal cells in tubular structures of both tumors were positive for keratin 19 with or without keratin 14. Nonluminal peripheral cells of pleomorphic adenomas were mostly positive for keratin 14, and a small fraction of them expressed smooth muscle proteins. Conversely, peripheral cells of adenoid cystic carcinomas were mostly positive for smooth muscle proteins, and some of them expressed keratin 14. These results strongly suggest (1) that the luminal cell progenitors transform into major constituents of pleomorphic adenoma cells with keratin 14 but not smooth muscle proteins, and (2) that the peripheral cells of adenoid cystic carcinoma are derived from undifferentiated MECs. Solid structures of pleomorphic adenomas were formed by proliferation of the peripheral cells. MECs were observed only occasionally in the periphery. Solid and cribriform structures of adenoid cystic carcinomas were formed by proliferation of the luminal cells. MECs were observed in the periphery and around the pseudocyst.

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Section: Discussionsupporting

confidence: 83%

Keratin 14 immunoreactive cells in pleomorphic adenomas and adenoid cystic carcinomas of salivary glands

et al. 2000

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“…Central to the problem is the relationship of intermediate cells to both goblet and epidermoid cells and whether myoepithelial or even modified myoepithelial cells are present. A number of immunohistochemical studies have indicated that at least in some mucoepidermoid carcinomas, neoplastic myoepithelial cells do differentiate (Hassanin et al, 1989;Nikai et al, 1986;Regezi et al, 1991). Ultrastructural evidence of myoepithelial participation has confirmed these observations and provided information on the relationship of the various cell types in mucoepidermoid carcinoma (Chaudhry et al, 1989;Dardick et al, 1984bDardick et al, , 1990b.…”

Section: Mucoepidermoid Carcinomamentioning

confidence: 89%

Pathology of the salivary glands: The contribution of electron microscopy

Dardick

Burford-Mason

1994

Microscopy Res & Technique

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Electron microscopy has a limited role in the diagnosis of primary salivary gland tumors, although it can be helpful in metastatic lesions of possible salivary gland origin. The diversity of subtypes in salivary gland tumors, as well as the range of histomorphology within any one subtype, is unparalleled in any other human tumor. This and their relative infrequency causes diagnostic problems for pathologists. Ultrastructural techniques have been of major importance in determining the inter-relationship of these tumors for classification purposes, revealing the subtle variations in common cellular differentiation pathways, determining the organization of tumor cells, and displaying the importance of extracellular matrix materials in establishing diagnostic criteria for each of the many subtypes. Electron microscopy has also been valuable in non-neoplastic salivary gland disease and has an increasing role in experimental studies involving tissue from human and animal salivary parenchyma.

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“…It is a useful means of determining if MEC are present in a neoplasm (Guccion and Redman, 1986) and for an overview of the distribution of MEC in neoplasms in which they occur Nikai et al, 1986). However, it does not distinguish between MEC and other cells with myofibrils, such as myofibroblasts, and so cannot resolve the origin of the stromal cells in pleomorphic adenoma.…”

Section: Identification Of Mec In Salivary Gland Neoplasmsmentioning

confidence: 99%

“…The TP-LFC stain delineates myofibrils, the fine filaments of striated ducts, and the terminal web in developing and mature salivary glands (Redman and Ball, 1979;Redman et al, 1980). It is a useful means of determining if MEC are present in a neoplasm (Guccion and and for an overview of the distribution of MEC in neoplasms in which they occur (El-Bardaie and Nikai et al, 1986). However, it does not distinguish between MEC and other cells with myofibrils, such as myofibroblasts, and so cannot resolve the origin of the stromal cells in pleomorphic adenoma.…”

Section: Identification Of Mec In Salivary Gland Neoplasmsmentioning

confidence: 99%

Myoepithelium of salivary glands

Redman¹

1994

Microscopy Res & Technique

154

146

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In salivary glands and other exocrine organs, there are starfish-shaped cells that lie between the basal lamina and the acinar and ductal cells. These have structural features of both epithelium and smooth muscle cells, and so are called myoepithelial cells. Their functions include contraction when the gland is stimulated to secrete, compressing or reinforcing the underlying parenchymal cells, thus aiding in the expulsion of saliva and preventing damage to the other cells. They also may aid in the propagation of secretory and other stimuli. Their common developmental origin with the basal cells of the larger ducts is displayed in the mature glands by shared structural and immunohistochemical features, but most such basal cells do not have the distinguishing features of myoepithelial cells, such as myofibrils. Although myoepithelial cells can be identified by light microscopy through enzyme histochemistry and special stains and immunohistochemistry for their myofibrils, these techniques can be misleading in salivary gland neoplasms. Thus, the most reliable means of identifying neoplastic myoepithelial cells is with a combination of histochemistry and electron microscopy. The extent to which these cells are derived from undifferentiated stem cells in both normal and neoplastic growth is controversial. The presentation here of transmission electron microscopy (TEM) of well-differentiated myoepithelial cells in mitotic division indicates that stem cells are not necessarily the only source of myoepithelial cells in the later stages of salivary gland development or in neoplasia.

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Histologic evaluation of myoepithelial participation in salivary gland tumors

Cited by 30 publications

References 15 publications

Keratin 14 immunoreactive cells in pleomorphic adenomas and adenoid cystic carcinomas of salivary glands

Keratin 14 immunoreactive cells in pleomorphic adenomas and adenoid cystic carcinomas of salivary glands

Pathology of the salivary glands: The contribution of electron microscopy

Myoepithelium of salivary glands

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