Histologic Study of an Autologous Fat Graft in the Larynx of Dogs With Unilateral Vocal Fold Paralysis

Kruschewsky, Leonardo de Souza; Mello-Filho, Francisco Veríssimo; Saggioro, Fabiano Pinto; Serafini, Luciano Neder; Rosen, C

doi:10.1097/mlg.0b013e3181373a4f

Cited by 7 publications

(8 citation statements)

References 21 publications

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“…Doing so in a controlled excised larynx setting with easily repeatable conditions decreased potential variability that may arise when conducting research on human subjects. Both ex vivo and in vivo canine larynges have been used previously to study interventions for vocal fold paralysis 35, 36, 38. There are several anatomical differences between the human and canine larynx.…”

Section: Discussionmentioning

confidence: 99%

“…IL led to improvements comparable to ML in acoustics and aerodynamics, but ML increased mucosal wave amplitude of the paralyzed fold, whereas IL significantly decreased it. Analyzing other injection materials, such as fat, which has the same viscosity as the vocal fold,11 may yield different results; however, a portion of these substances may be absorbed by the surrounding tissue over time 38, 41, 42…”

Section: Discussionmentioning

confidence: 99%

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Multiparameter comparison of injection laryngoplasty, medialization laryngoplasty, and arytenoid adduction in an excised larynx model

et al. 2010

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Objective-Evaluate the effect of injection laryngoplasty (IL), medialization laryngoplasty (ML), and ML combined with arytenoid adduction (ML-AA) on acoustic, aerodynamic, and mucosal wave measurements in an excised larynx setup.Methods-Measurements were recorded for eight excised canine larynges with simulated unilateral vocal fold paralysis (UVFP) before and after vocal fold injection with Cymetra. A second set of eight larynges was used to evaluate medialization laryngoplasty using a Silastic implant without and with arytenoid adduction.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Multiparameter comparison of injection laryngoplasty, medialization laryngoplasty, and arytenoid adduction in an excised larynx model

et al. 2010

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“…As the size and histologic properties of the canine and human larynx are similar,16 it is an appropriate model for studying human laryngeal physiology. Both ex vivo and in vivo canine larynges have been used previously to study interventions for vocal fold paralysis 16–18. There are several anatomic differences between the human and canine larynx.…”

Section: Methodsmentioning

confidence: 99%

Preliminary investigation of adjustable balloon implant for type I thyroplasty

Hoffman¹,

Witt²,

McCulloch³

et al. 2011

The Laryngoscope

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Objective-We present the adjustable balloon implant (ABI), a novel implant to be used in type I thyroplasty for the treatment of vocal fold paralysis. The ABI offers the same medialization provided by other implants, but can easily be catered to individual patient anatomy as well as modified postoperatively without the need for a revision thyroplasty. Study design-Repeated measures with each larynx serving as its own control.Methods-Medialization thyroplasty (MT) with the ABI was performed on five excised canine larynges. Mucosal wave, aerodynamic, and acoustic parameters were measured for three conditions: normal; right vocal fold paralysis; and paralysis with the ABI.Results-Insertion of the ABI resulted in significant decreases in both phonation threshold pressure and phonation threshold flow. Perturbation parameters of percent jitter and percent shimmer were also significantly decreased and restored to normal levels. Signal-to-noise ratio was significantly increased to the normal level as well. The mucosal wave was preserved after implant insertion.Conclusions-This preliminary experiment showing significant improvements in aerodynamic and acoustic parameters demonstrates the potential of the ABI as a thyroplasty implant. Effective medialization and preservation of the mucosal wave combined with post-operative adjustability makes it a potentially valuable clinical device.

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“…Six larynges were excised postmortem from canines sacrificed for unrelated purposes according to the protocol described by Jiang and Titze . Canine larynges have been used extensively to study laryngeal physiology including vocal fold paralysis . Following visual inspection to ensure no signs of trauma or disorders were present, larynges were frozen in 0.9% saline.…”

Section: Methodsmentioning

confidence: 99%

“…12 Canine larynges have been used extensively to study laryngeal physiology including vocal fold paralysis. [13][14][15] Following visual inspection to ensure no signs of trauma or disorders were present, larynges were frozen in 0.9% saline.…”

Section: Laryngesmentioning

confidence: 99%

Excised larynx evaluation of wedge‐shaped adjustable balloon implant for minimally invasive type I thyroplasty

et al. 2013

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Objectives/Hypothesis To describe the method of inserting a wedge-shaped adjustable balloon implant (wABI) via a minithyrotomy for medialization thyroplasty and evaluate its effect on a range of phonatory parameters using the excised larynx bench apparatus. Study Design Repeated measures with each larynx serving as its own control. Methods A prototype wABI was deployed in six excised canine larynges of various sizes through a minithyrotomy and then filled with saline. Mucosal wave, aerodynamic, and acoustic parameters were measured for three conditions: normal, vocal fold paralysis, and paralysis with the wABI. Results Phonation threshold pressure (P <.001), flow (P <.001), and power (P =.002) were significantly lower for wABI compared to paralysis trials; values did not differ significantly from normal trials. Percent jitter (P =.002) and percent shimmer (P =.007) were also significantly decreased compared to the paralysis condition, and values were not significantly different compared to normal. The mucosal wave was preserved after insertion of the wABI. Conclusions Effective vocal fold medialization with preservation of the mucosal wave was observed with the wABI in this preliminary excised larynx experiment. The wABI offers the potential for a minimally invasive insertion in addition to postoperative adjustability. Further studies in living animals and humans are warranted to evaluate clinical utility.

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Histologic Study of an Autologous Fat Graft in the Larynx of Dogs With Unilateral Vocal Fold Paralysis

Abstract: The histologic findings for the AFG applied to a vocal fold were similar in groups VFL-SGA, VFLI-SGB and FGML-SGC 12 weeks after grafting, with no statistically significant differences between groups, and revealed an almost total loss of the grafted fat.

Cited by 7 publications

References 21 publications

Multiparameter comparison of injection laryngoplasty, medialization laryngoplasty, and arytenoid adduction in an excised larynx model

Multiparameter comparison of injection laryngoplasty, medialization laryngoplasty, and arytenoid adduction in an excised larynx model

Preliminary investigation of adjustable balloon implant for type I thyroplasty

Excised larynx evaluation of wedge‐shaped adjustable balloon implant for minimally invasive type I thyroplasty

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