2013
DOI: 10.1371/journal.pone.0053468
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Histological Analysis and 3D Reconstruction of Winter Cereal Crowns Recovering from Freezing: A Unique Response in Oat (Avena sativa L.)

Abstract: The crown is the below ground portion of the stem of a grass which contains meristematic cells that give rise to new shoots and roots following winter. To better understand mechanisms of survival from freezing, a histological analysis was performed on rye, wheat, barley and oat plants that had been frozen, thawed and allowed to resume growth under controlled conditions. Extensive tissue disruption and abnormal cell structure was noticed in the center of the crown of all 4 species with relatively normal cells o… Show more

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Cited by 24 publications
(35 citation statements)
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“…Ice sectioning of cold‐acclimated Norstar and Hazlet crowns cooled to −10°C visually showed the formation of cavities in the VTZ (Figure ). This corroborated previous reports by Livingston and colleagues who observed the formation of large cavities in three dimensional reconstructions of injured oat crowns (Livingston & Tuong, ; Livingston et al, ). Sectioning of Norstar or Hazlet crowns cooled to −10°C did not detect any visible ice near the SAM.…”
Section: Resultssupporting
confidence: 92%
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“…Ice sectioning of cold‐acclimated Norstar and Hazlet crowns cooled to −10°C visually showed the formation of cavities in the VTZ (Figure ). This corroborated previous reports by Livingston and colleagues who observed the formation of large cavities in three dimensional reconstructions of injured oat crowns (Livingston & Tuong, ; Livingston et al, ). Sectioning of Norstar or Hazlet crowns cooled to −10°C did not detect any visible ice near the SAM.…”
Section: Resultssupporting
confidence: 92%
“…In cold‐acclimated crown tissues, in vitro freezing led to the VTZ having a higher EL 50 compared with the SAM. This was the opposite order of injury observed from in vivo whole‐crown freezing studies using tetrazolium chloride viability staining (Livingston et al, ; Tanino & McKersie, ; Willick et al, ), histological analysis (Livingston et al, ), and visible crown injury (Chen et al, ; Olien, ). Cold‐acclimated SAM frozen in vitro does not have a tissue ice sink to facilitate water withdrawal.…”
Section: Resultsmentioning
confidence: 89%
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“…The coefficients of determination of arginine versus DAF were the highest observed (Table 1), suggesting a very close relationship between response to freezing, free arginine pools, and the formation of the spherical structure in the interior of frozen tillers [31]. A study of metabolic changes in populations of Arabidopsis lyrata ssp.…”
Section: Resultsmentioning
confidence: 86%
“…We have previously reported the development of a spherical mass of safranin staining tissue over the two weeks following freezing of oat crowns [31]. Those tissues were produced using the same protocol as used in this metabolomics study.…”
Section: Resultsmentioning
confidence: 99%