Histological analysis of biocompatibility of ionomer cements with an acid-base reaction

Abstract

histological analysis of biocompatibility of ionomer cements with an acid-base reaction abstract: The purpose of this study was to evaluate the inflammatory and cure events of acid-based reactions using glass ionomer cement used for cementation of crowns, bridges, onlays and orthodontic bands implanted in subcutaneous tissue, at different time intervals. A total of 48 male Wistar rats were used, distributed into 4 groups (n = 12), as follows: Group C (control, polyethylene), Group ME (Meron), Group KC (Ketac Cem) and Group PR (Precedent). The animals were sacrificed after time intervals of 7, 15 and 30 days, and their tissues were analyzed under an optical microscope for such events as inflammatory infiltrate, edema, necrosis, granulation tissue, multinucleated giant cells, young fibroblasts and collagen. The results was assessed using Kruskal-Wallis and Dunn's tests (p < 0.05). In the initial period, intense inflammatory infiltrate was observed for all the materials with no significant difference among them (p = 0.104). Groups PR and KC showed significant difference in relation to Group C, at 7 days (p = 0.025) and 15 days (p = 0.006). Edema and giant cells were more expressive in Group ME, differing significantly from Groups C (p = 0.023) and KC (p = 0.039), respectively, at 7 days. Group ME showed a statistically significant difference in relation to Groups PR and KC for the presence of young fibroblasts (p = 0.009) and for collagen (p = 0.002), at 7 days. Within the limits of this in vivo study, Precedent and Ketac Cem glass ionomer cements showed better tissue healing with a greater number of fibroblasts and collagen, as compared to Meron.

Effect of Chenopodium ambrosioides on the healing process of the in vivo bone tissue

“…The rats were anesthetized with an injection of ketamine (Konig S.A., São Paulo, SP, Brazil) and xylazine (Konig S.A., São Paulo, SP, Brazil) administered intramuscularly (Pinto et al, ). Subsequently, trichotomy of the surgical area and local asepsis with 4% chlorhexidine digluconate were performed (Dos Santos et al, ; Santos et al, ).After this, a straight line incision measuring approximately 20 mm was made with a No. 15 scalpel blade (Embramac, Itapira, Brazil) adapted to a scalpel handle, until the periosteum was reached.…”

Evaluation of the cytotoxicity of selected conventional glass ionomer cements on human gingival fibroblasts

“…9 Many authors suggest their high biocompatibility. [10][11][12][13] However, there are reports noting an adverse effect of these materials on live cells.…”

Biocompatibility of a New Dental Glass Ionomer Cement with Cellulose Microfibers and Cellulose Nanocrystals

“…The complete absence of these cells indicated that the biomaterials had low potential to induce foreign body giant cell reactions. This type of granulomatous tissue reaction has been related for resin-modified glass ionomer cement (17) and some ionomer cements with an acid-base reaction (22,23).…”