Histological Analysis of Endocrine Disruptive Effects in Small Laboratory Fish

Dietrich, Daniel R.; Krieger, Heiko O.

doi:10.1002/9780470431795

Cited by 70 publications

(113 citation statements)

References 28 publications

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“…Histopathological biomarkers in gonads can help to understand the mode of action of chemicals on processes related to reproduction (Au et al, 2001;Dietrich et al, 2009). In a recent study, severe histopathological alterations were observed in gonads of female Psammechinus miliaris following exposure to phenanthrene (Schäfer and Köhler, 2009), a low molecular weight, 3-ring polycyclic aromatic hydrocarbon (PAH), and a major component of total PAH compounds in marine waters (Law et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…The second histopathological parameter, fibrosis, is often described to occur in reproductive tissues of fish after exposure to environmental contaminants (Dietrich et al, 2009). Fibrosis involves augmentation of collagen and more massive appearance of the connective tissue (Dietrich et al, 2009), and phenanthrene exposure increases fibrosis in sea urchin gonads (Schäfer and Köhler, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Another histopathological parameter, easy to record in females, is the percentage of atretic oocytes in the ovaries. Atresia is a degenerative and resorptive process of oocytes which may become pathologic after chemical exposure (Blazer, 2002;Dietrich et al, 2009;Schäfer and Köhler, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Sex-specific biochemical and histological differences in gonads of sea urchins (Psammechinus miliaris) and their response to phenanthrene exposure

Schäfer

Abele

Weihe

et al. 2011

Marine Environmental Research

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a b s t r a c tFemale and male individuals of the same species often differ with respect to their susceptibility to toxicant stress. In the present study, sea urchins (Psammechinus miliaris) of both sexes were exposed to high (150 mg L À1 ) and environmentally relevant (5 mg L À1 ) concentrations of phenanthrene over 10 days. While food intake was significantly decreased following exposure to 150 mg L À1 phenanthrene, histological indices (lipofuscin accumulation, fibrosis, oocyte atresia), energetic status (energy charge, sum adenylates, AMP/ATP ratio) as well as ascorbate levels in the gonads showed either little or no effect upon phenanthrene exposure. However, most parameters (vitamin C, energy charge, sum adenylates, AMP/ATP ratio, ATP and ADP concentrations, lipofuscin content, fibrosis) significantly differed between male and female animals. This study illustrates the difficulties to identify toxic injury in reproductive tissue as it may be superimposed by gametogenesis and spawning of gametes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sex-specific biochemical and histological differences in gonads of sea urchins (Psammechinus miliaris) and their response to phenanthrene exposure

Schäfer

Abele

Weihe

et al. 2011

Marine Environmental Research

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“…Slides were examined for any abnormalities at the microscopic level. Sections of gonads were used to confirm sex, stage of development, percent atretic eggs, and any other abnormalities such as occurrence of intersex, fibrosis, abnormal yolk accumulation, ceroid/lipofuscin accumulations, or Sertoli cell proliferation and were evaluated after neoplasia as described by Blazer (2002), Leino et al (2005), and Dietrich and Krieger (2009).…”

Section: Gonad Histologymentioning

confidence: 99%

“…Numerous histopathological changes in tissues have been used as biomarkers of exposure and (or) environmental stress, including neoplasms and preneoplastic changes of liver and skin (Myers and Fournie, 2002;Stentiford et al, 2003;Au, 2004;Lyons et al, 2004). Histopathology of the gonads was used to verify sex and stage of gonadal development, as well as identify abnormalities such as testicular oocytes (Blazer, 2002;Dietrich and Krieger, 2009). Although effects on the reproductive system have been the most commonly documented, other systems can be affected by exposure to CECs and legacy contaminants.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of wild fish health at selected sites in the Great Lakes Basin: methods and preliminary results

Blazer¹,

Mazik²,

Iwanowicz³

et al. 2014

Open-File Report

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For more information on the USGS-the Federal source for science about the Earth, its natural and living resources, natural hazards, and the environment-visit http://www.usgs.gov or call 1-888-ASK-USGS (1-888-275-8747) For an overview of USGS information products, including maps, imagery, and publications, visit http: //www.usgs.gov/pubprod To order this and other USGS information products, visit http://store.usgs.gov Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.Although this information product, for the most part, is in the public domain, it also may contain copyrighted materials as noted in the text. Permission to reproduce copyrighted items must be secured from the copyright owner.This draft manuscript is distributed solely for purposes of scientific peer review. Its content is deliberative and predecisional, so it must not be disclosed or released by reviewers. Because the manuscript has not yet been approved for publication by the U.S. Geological Survey, it does not represent any official USGS finding or policy. AbstractDuring fall 2010 and spring 2011, a total of 119 brown bullhead (Ameiurus nebulosus), 136 white sucker (Catostomus commersoni), 73 smallmouth bass (Micropterus dolomieu), and 59 largemouth bass (M. salmoides) were collected from seven Great Lakes Basin Areas of Concern and one Reference Site. Comprehensive fish health assessments were conducted in order to document potential adverse effects from exposure to complex chemical mixtures. Fish were necropsied on site, blood samples obtained, pieces of liver, spleen, kidney, gill and any abnormalities placed in fixative for histopathology. Liver samples were saved for gene expression analysis and otoliths were removed for aging. A suite of fish health indicators was developed and implemented for site comparisons and to document seasonal effects and species differences in response to environmental conditions. Organism level (grossly visible lesions, condition factor), tissue level (microscopic pathology, organosomatic indices, micronuclei, and other nuclear abnormalities), plasma factors (reproductive steroid hormones, vitellogenin), and molecular (gene expression) indicators were included. This report describes the methods and preliminary results.

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Histopathologic Prevalence and Severity of Testicular Oocytes in Smallmouth Bass from Two Archival Collections

et al. 2020

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During recent decades, survey studies have documented the widespread presence of oocytes in the testes of male Smallmouth Bass Micropterus dolomieu collected from surface waters throughout the United States. There are few published reports of testicular oocytes (TO) in Smallmouth Bass before the 1990s, so it is difficult to know how long this has been occurring. Consequently, this study was conducted to evaluate the prevalence and severity of TO occurrence in whole fish specimens from two archival collections—the Smithsonian Institution's National Museum of Natural History in Suitland, Maryland, and Cornell University's Museum of Vertebrates in Ithaca, New York. Gonads were excised from 167 preserved male Smallmouth Bass that were originally collected between 1875 and 2004, and routine histologic sections were prepared and examined. The severity of TO was determined using a semiquantitative scoring system. Overall, 52.1% of male Smallmouth Bass were found to have TO. Affected fish had been collected in 11 of the 18 represented states, and TO were found in specimens harvested during decades as early as the 1880s and 1900s. Unfortunately, the small number of samples acquired at the earliest time periods precluded analyses of prevalence and severity trends over time. The results of this study demonstrated that the phenomenon of TO in male Smallmouth Bass is at least a century old and confirmed the widespread nature of this finding throughout the species’ historic range. Further research efforts should focus on determining the baseline prevalence of TO in laboratory‐reared male Smallmouth Bass that have not been exposed to endocrine active substances or the effects of experimental estrogen exposure on such fish.

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Histological Analysis of Endocrine Disruptive Effects in Small Laboratory Fish

Cited by 70 publications

References 28 publications

Sex-specific biochemical and histological differences in gonads of sea urchins (Psammechinus miliaris) and their response to phenanthrene exposure

Sex-specific biochemical and histological differences in gonads of sea urchins (Psammechinus miliaris) and their response to phenanthrene exposure

Monitoring of wild fish health at selected sites in the Great Lakes Basin: methods and preliminary results

Histopathologic Prevalence and Severity of Testicular Oocytes in Smallmouth Bass from Two Archival Collections

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