Histological analysis of femoral bones in rabbits administered by amygdalin

Abstract

Cyanogenic glycosides are present in several economically important plant foods. Amygdalin, one of the most common cyanoglucoside, can be found abundantly in the seeds of apples, bitter almonds, apricots, peaches, various beans, cereals, cassava and sorghum. Amygdalin has been used for the treatment of cancer, it shows killing effects on cancer cells by release of cyanide. However, its effect on bone structure has not been investigated to date. Therefore, the objective of this study was to determine a possible effect of amygdalin application on femoral bone microstructure in adult rabbits. Four month old rabbits were randomly divided into two groups of three animals each. Rabbits from E group received amygdalin intramuscularly at a dose 0.6 mg.kg-1 body weight (bw) (group E, n = 3) one time per day during 28 days. The second group of rabbits without amygdalin supplementation served as a control (group C, n = 3). After 28 days, histological structure of femoral bones in both groups of rabbits was analysed and compared. Rabbits from E group displayed different microstructure in middle part of the compact bone and near endosteal bone surface. For endosteal border, an absence of the primary vascular longitudinal bone tissue was typical. This part of the bone was formed by irregular Haversian and/or by dense Haversian bone tissues. In the middle part of substantia compacta, primary vascular longitudinal bone tissue was observed. Cortical bone thickness did not change between rabbits from E and C groups. However, rabbits from E group had a significantly lower values of primary osteons' vascular canals and secondary osteons as compared to the C group. On the other hand, all measured parameters of Haversian canals did not differ between rabbits from both groups. Our results demonstrate that intramuscular application of amygdalin at the dose used in our study affects femoral bone microstructure in rabbits.

Human Urine Alterations Caused by Apricot Seeds Consumption

“…Amygdalin is also a potential antifibrotic agent that may have therapeutic potential for patients with fibrotic kidney disease [29]. Kovacova et al [30] observed significantly lower values of primary osteons' vascular canals and secondary osteons in femoral bones of rabbits administered by amygdalin. Our previous in vivo study on rabbits showed no obvious effect of intramuscular administration of amygdalin (0.6 and 3.0 mg/kg) on energy profile [31].…”

Nutritional and toxicological composition analysis of selected cassava processed products

“…Under tropical conditions cyanogenic glucoside readily hydrolysed liberating HCN (Udedibie et al, 2008). However, there are some other compounds in cassava such as amygladin which form cyanide as well (Kováčová et al, 2016 Although Manihot esculanta tubers are widely consumed in Sri Lanka for years, there is, to our knowledge, no much available data concerning the nutritional composition and toxicological content of these tubers. The purpose of the study was to determine nutritional composition and cyanide content as a toxicological factor in MU51 variety and its selected processed products (boiled, flour, starch, chips) and to determine if there were any statistical differences in the nutrient content of fresh MU 51 tubers due to its processing method and to determine the variation of toxicological content between fresh MU 51 tubers and its processed conditions: boiled, chips, flour and starch products.…”

Oral and intramuscular application of cyanogenic glycoside amygdalin did not induce changes in haematological profile of male rabbits

“…However, its effect on animal and human organisms is still not clear. (Nabavizadeh et al, 2011;Kovacova et al, 2016). It has been used as a traditional drug because of its wide range of medicinal benefits, including curing or preventing cancer, relieving fever, suppressing cough, and quenching thirst (Zhou et al, 2012).…”