2017
DOI: 10.1007/978-1-4939-7156-5_13
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Histological Analysis of the Developmental Stages of Direct Somatic Embryogenesis Induced from In Vitro Leaf Explants of Date Palm

Abstract: Somatic embryogenesis is an ideal technique for the micropropagation of date palm using different explant tissue; however, histological studies describing the ontogenesis of plant regeneration are limited. This chapter provides a simple protocol for the histological analysis of the successive developmental stages of direct somatic embryogenesis induced from in vitro leaf explants. Direct somatic embryos are obtained from Murashige and Skoog (MS) medium containing 2 mg/L 6-benzylaminopurine. In order to observe… Show more

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Cited by 5 publications
(2 citation statements)
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“…Semi-thin sections (1 µm) were made using a glass knife and stained with 0.03% toluidine blue. The successive morphogenesis stages (globular, bipolar and FDE) of somatic embryos were fixed in FAA solution (formaldehyde, acetic acid and 50% ethyl alcohol, 5:5:90 by volume) for 24 h. Progressive dehydration using ascending concentrations of ethanol was performed and then embedding in Paraplast (Sigma Aldrich, St. Louis, MA, USA, Paraplast plus p. 3688), according to Abdelbar [23]. Transverse and longitudinal sections 8 µm in thickness were made using a LEICA rotary microtome, model RM 2125 RTS, and stained with a safranin-fast green combination [24].…”
Section: Histological Analysesmentioning
confidence: 99%
“…Semi-thin sections (1 µm) were made using a glass knife and stained with 0.03% toluidine blue. The successive morphogenesis stages (globular, bipolar and FDE) of somatic embryos were fixed in FAA solution (formaldehyde, acetic acid and 50% ethyl alcohol, 5:5:90 by volume) for 24 h. Progressive dehydration using ascending concentrations of ethanol was performed and then embedding in Paraplast (Sigma Aldrich, St. Louis, MA, USA, Paraplast plus p. 3688), according to Abdelbar [23]. Transverse and longitudinal sections 8 µm in thickness were made using a LEICA rotary microtome, model RM 2125 RTS, and stained with a safranin-fast green combination [24].…”
Section: Histological Analysesmentioning
confidence: 99%
“…Previous date palm micropropagation protocols determined the incubation under light for somatic embryo germination and conversion to plantlets. (Almusawi, 2015;Abdelbar, 2017;Zayed, 2017;Fki et al, 2017).…”
Section: Introductionmentioning
confidence: 99%