Histological and genotoxic evaluation of gold nanoparticles in ovarian cells of zebrafish (Danio rerio)

Dayal, Navami; Thakur, Mansee; Patil, Poonam; Singh, Dipty; Vanage, Geeta; Joshi, Deeksha

doi:10.1007/s11051-016-3549-0

Cited by 18 publications

(7 citation statements)

References 37 publications

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“…In this study, the possible mechanism is Ag-NPs passed through the gonads and generated ROS in spermatocytes and internal germ cells, inducing the early germ cell death that led to the cells and DNA damage. The genetic integrity of the gonads is an essential aspect of reproductive success [63]. Thus, as ROS can harm the DNA and cells, this damaged genetic will be inherited to the next generation as the defective genetic [64,65].…”

Section: Reproductive Toxicity Of Ps-npsmentioning

confidence: 99%

Nanoplastics Cause Neurobehavioral Impairments, Reproductive and Oxidative Damages, and Biomarker Responses in Zebrafish: Throwing up Alarms of Wide Spread Health Risk of Exposure

Sarasamma

Audira

Siregar

et al. 2020

IJMS

272

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Plastic pollution is a growing global emergency and it could serve as a geological indicator of the Anthropocene era. Microplastics are potentially more hazardous than macroplastics, as the former can permeate biological membranes. The toxicity of microplastic exposure on humans and aquatic organisms has been documented, but the toxicity and behavioral changes of nanoplastics (NPs) in mammals are scarce. In spite of their small size, nanoplastics have an enormous surface area, which bears the potential to bind even bigger amounts of toxic compounds in comparison to microplastics. Here, we used polystyrene nanoplastics (PS-NPs) (diameter size at ~70 nm) to investigate the neurobehavioral alterations, tissue distribution, accumulation, and specific health risk of nanoplastics in adult zebrafish. The results demonstrated that PS-NPs accumulated in gonads, intestine, liver, and brain with a tissue distribution pattern that was greatly dependent on the size and shape of the NPs particle. Importantly, an analysis of multiple behavior endpoints and different biochemical biomarkers evidenced that PS-NPs exposure induced disturbance of lipid and energy metabolism as well as oxidative stress and tissue accumulation. Pronounced behavior alterations in their locomotion activity, aggressiveness, shoal formation, and predator avoidance behavior were exhibited by the high concentration of the PS-NPs group, along with the dysregulated circadian rhythm locomotion activity after its chronic exposure. Moreover, several important neurotransmitter biomarkers for neurotoxicity investigation were significantly altered after one week of PS-NPs exposure and these significant changes may indicate the potential toxicity from PS-NPs exposure. In addition, after ~1-month incubation, the fluorescence spectroscopy results revealed the accumulation and distribution of PS-NPs across zebrafish tissues, especially in gonads, which would possibly further affect fish reproductive function. Overall, our results provided new evidence for the adverse consequences of PS-NPs-induced behavioral dysregulation and changes at the molecular level that eventually reduce the survival fitness of zebrafish in the ecosystem.

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Section: Reproductive Toxicity Of Ps-npsmentioning

confidence: 99%

Nanoplastics Cause Neurobehavioral Impairments, Reproductive and Oxidative Damages, and Biomarker Responses in Zebrafish: Throwing up Alarms of Wide Spread Health Risk of Exposure

Sarasamma

Audira

Siregar

et al. 2020

IJMS

272

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“…Toxicological studies have demonstrated the reproductive risks from ENMs in fish and sea urchins, inducing damage to ovarian and testicular tissues, oocytes, and sperms. [212][213][214][215] However, these studies did not consider the altered reproductive effects from an environmental corona on ENMs, nor investigate changes in the offspring from ENM-treated parents. A recent study reported that protein corona on Ag NPs formed in the plasma of Micropterus dolomieu varied with fish sex, where the corona in male plasma was slightly thinner and had a less negative ζ−potential than the female plasma.…”

Section: Genotoxicity and Reproductive Toxicitymentioning

confidence: 99%

The Crucial Role of Environmental Coronas in Determining the Biological Effects of Engineered Nanomaterials

Wang

et al. 2020

Small

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In aquatic environments, a large number of ecological macromolecules (e.g., natural organic matter (NOM), extracellular polymeric substances (EPS), and proteins) can adsorb onto the surface of engineered nanomaterials (ENMs) to form a unique environmental corona. The presence of environmental corona as an eco–nano interface can significantly alter the bioavailability, biocompatibility, and toxicity of pristine ENMs to aquatic organisms. However, as an emerging field, research on the impact of the environmental corona on the fate and behavior of ENMs in aquatic environments is still in its infancy. To promote a deeper understanding of its importance in driving or moderating ENM toxicity, this study systemically recapitulates the literature of representative types of macromolecules that are adsorbed onto ENMs; these constitute the environmental corona, including NOM, EPS, proteins, and surfactants. Next, the ecotoxicological effects of environmental corona‐coated ENMs on representative aquatic organisms at different trophic levels are discussed in comparison to pristine ENMs, based on the reported studies. According to this analysis, molecular mechanisms triggered by pristine and environmental corona‐coated ENMs are compared, including membrane adhesion, membrane damage, cellular internalization, oxidative stress, immunotoxicity, genotoxicity, and reproductive toxicity. Finally, current knowledge gaps and challenges in this field are discussed from the ecotoxicology perspective.

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“…Thanks to the spawning procedure on the last day of the experiment, it is known that the oocytes observed in the gonad were formed during the seven-day exposure to the tested nanoxenobiotics (zebrafish is a model species with continuous and aseasonal reproduction under laboratory conditions). Dayal et al [ 36 ] observed in histopathological changes in the oocytes of ovaries from zebrafish exposed to a chronic 28-day exposure of gold nanoparticles (AuNPs), such as the detachment of zona radiata from the oocyte, appearance of atretic oocytes, and DNA damage in the ovarian cells. The results of this study showed that nanoxenobiotics also induce cytotoxicity and genotoxicity in the female gonad and cause endocrine disruption.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Seven-Day Exposure to Silver Nanoparticles on Fertility and Homeostasis of Zebrafish (Danio rerio)

Szudrowicz

Kamaszewski

Adamski

et al. 2022

IJMS

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Silver nanoparticles (AgNPs) are found in open waters, but the effect of their low concentrations on an organism’s homeostasis is not fully understood. The aim of the study was to determine the short-term exposure effects of AgNPs coated by PvP (polyvinylpyrrolidone) on the homeostasis of livers and gonads in zebrafish. Sexually mature zebrafish were exposed for seven days to silver ions (0.01 mg/dm3) or AgNPs (0.01; 0.05; 0.1; 0.5; 1.0 mg/dm3). On the last day, the liver, testes, and ovaries were subjected to a histology analysis. In the liver, we analyzed the expression of the cat, gpx1a, gsr, sod1, and cyp1a genes. On the last day of the experiment, the lowest survival rate was found in the AgNPs 0.05 mg/dm3 group. The histological analysis showed that AgNPs and silver ions cause an increase in the area of hepatocytes. The highest proliferation index of hepatocytes was found in the AgNP 0.05 mg/dm3 group. Furthermore, AgNPs were found to interfere with spermatogenesis and oogonesis as well as reduce the expression levels of the cat, gpx1a, and sod1 genes in the liver compared with the control group. Based on the results, it can be concluded that exposure to AgNPs causes cytotoxic changes in zebrafish, activates the immune system, negatively affects the process of meiosis in the gonads, and generates oxidative stress.

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Histological and genotoxic evaluation of gold nanoparticles in ovarian cells of zebrafish (Danio rerio)

Cited by 18 publications

References 37 publications

Nanoplastics Cause Neurobehavioral Impairments, Reproductive and Oxidative Damages, and Biomarker Responses in Zebrafish: Throwing up Alarms of Wide Spread Health Risk of Exposure

Nanoplastics Cause Neurobehavioral Impairments, Reproductive and Oxidative Damages, and Biomarker Responses in Zebrafish: Throwing up Alarms of Wide Spread Health Risk of Exposure

The Crucial Role of Environmental Coronas in Determining the Biological Effects of Engineered Nanomaterials

The Effects of Seven-Day Exposure to Silver Nanoparticles on Fertility and Homeostasis of Zebrafish (Danio rerio)

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